Separation of histidine enantiomers by capillary electrochromatography with molecularly imprinted monolithic columns

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dc.authorid0000-0001-8969-2389en_US
dc.authorid0000-0002-6672-6862en_US
dc.authorid0000-0003-0177-5134en_US
dc.authorid0000-0003-3260-1639
dc.contributor.authorŞarkaya, Koray
dc.contributor.authorAşır, Süleyman
dc.contributor.authorGöktürk, Ilgım
dc.contributor.authorEktirici, Sisem
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorYavuz, Handan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:54:11Z
dc.date.available2021-06-23T19:54:11Z
dc.date.issued2020
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractIn this study, we aimed to separate the enantiomeric forms of d,l-histidine, one of the essential amino acids, through molecular imprinted monolithic capillary electrochromatography columns prepared using hydrophobic N-methacryloyl-(l)-phenylalanine methyl ester as the functional monomer, and l-histidine as the template molecule. We investigated the effect of monomer ratio, temperature, template molecule mole ratio, crosslinker ratio, and porogen ratio to improve the permeability properties of the monolithic column. Characterization studies of the column were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy, scanning electron microscopy, and Brunauer-Emmett-Teller analysis. The chromatographic performance of the column was investigated using alkylbenzene-derived compounds. We evaluated some parameters to determine the optimum conditions for electrochromatographic studies such as electric field, organic solvent ratio, and pressure effect. The calculated imprinting factor (2.18) proved that the l-histidine imprinted amino acid-based monolithic column separated d,l-histidine molecules efficiently (percent relative standard deviation < 1.5) from each other using molecular imprinting technique with high-resolution values (resolution value > 1.5). As a result, selective d,l-histidine separation was achieved in less than 10 min at pH 7.0 without using a ligand or extra modification step for the first time.en_US
dc.identifier.doi10.1002/sscp.201900101
dc.identifier.endpage245en_US
dc.identifier.issn2573-1815
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85125150326en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage235en_US
dc.identifier.urihttps://doi.org/10.1002/sscp.201900101
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10451
dc.identifier.volume3en_US
dc.identifier.wosWOS:000541134200006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofSeparation Science Plusen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCapillary Electrochromatographyen_US
dc.subjectD,L-histidine Separationen_US
dc.subjectEnantiomeric Separationen_US
dc.subjectMolecular Imprintingen_US
dc.titleSeparation of histidine enantiomers by capillary electrochromatography with molecularly imprinted monolithic columnsen_US
dc.typeArticleen_US

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