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    Bioremediation of pesticides using microbial consortium: Challenges and future perspectives
    (Springer International Publishing, 2022) Yilmaz, Gaye Ezgi; Göktürk, Ilgim; Safran, Volkan; Yilmaz, Fatma; Denizli, Adil
    The widespread use of pesticides causes serious environmental and health problems. Pesticide use not only degrades soil quality but also enters the aquatic environment, so decontamination of pesticide-contaminated areas is a very complex process. The conventional methods used to remove polluting chemicals from the environment are not sufficient for the removal of pesticides. New technologies such as environmentally friendly, economical, and versatile bioremediation methods are required that take advantage of the ability of microorganisms to remove pollution from the environment. The use of microbial consortia has very important advantages in the bioremediation of pollution caused by pesticides. In this chapter, recent applications of microbial consortia used in pesticide bioremediation are discussed. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
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    Cellulose-based nanobiosorbents: An insight
    (Elsevier, 2022) Göktürk, Ilgim; Çimen, Duygu; Özbek, Merve Asena; Yilmaz, Fatma; Denizli, Adil
    The increasing world population and ever-growing industrialization cause more toxic heavy metals and organic pollutants to mix into natural resources than ever before. Simple and cost-effective methods based on adsorption applicable in many parts of the world are widely used to remove various pollutants from different sources. Cellulose, a natural polysaccharide, has excellent mechanical properties, high surface area, and it can be modified with specific functional groups, such as carboxyl, amino, sulfo groups, and cyclodextrin. Recently, nanocellulose and its applications have attracted high interest in both research and industrial areas due to their sustainability, availability, environmentally friendly, and remarkable applications in various fields. This chapter describes the recent advances in the production and application of cellulose-based nanomaterials used as bioadsorbents. Cellulose nanomaterials including cellulose nanocrystals and cellulose nanofibrils are produced using different preparation processes resulting in materials with unique structures and physicochemical properties and are examined in various fields such as biomedical, sensors, wastewater treatment, paper and board/packaging industry. In this chapter, the information about nanocellulose and its types and the recent developments of the cellulose-based nanobioadsorbents's environmental applications are comprehensively discussed. We emphasize the current trends and future remarks based on the environmental applications of cellulose-based nanomaterials. © 2022 Elsevier Inc. All rights reserved.
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    The Detection of Chemical and Biological Threat Agents Using Nanomaterial-Based Plasmonic Nanosensors
    (CRC Press, 2024) Aşir, Süleyman; Türkmen, Deniz; Ovezova, Mamajan; Göktürk, Ilgim; Yilmaz, Gaye Ezgi; Yilmaz, Fatma
    It is important to detect chemical and biological threat agents for homeland security and defense, and new detection methods with intelligent capabilities need to be developed. The hazards caused by biological and chemical threat agents and the limited availability of modern methods for detecting these agents form the basis of the need to develop new sensors. In recent years, nanomaterial-based plasmonic sensors have been widely used in applications for detecting chemical and biological threat agents due to their advantages. The large surface area to volume ratio of nanomaterials has enabled many possibilities for the creation of new nanosensors to be used for sensing. Herein, we provide an overview of biological and chemical threat agents. After giving a brief overview of the working principles of plasmonic sensors, the applications used to detect a broad spectrum of biological and chemical threat agents is extensively discussed. The final section summarizes concluding remarks about the identification of biological and chemical threat agents using nanomaterial-based plasmonic nanosensors and future perspectives. © 2024 selection and editorial matter, Adil Denizli; individual chapters, the contributors.
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    RNA purification
    (CRC Press, 2024) Göktürk, Ilgim; Saylan, Yeseren; Yilmaz, Fatma; Denizli, Adil
    Ribonucleic acid (RNA) molecules are used in various downstream experiments, including cloning, real-time reverse transcription polymerase chain reaction (RT-PCR), reverse transcription, and RNA-sequencing for gene expression analyses, which all require RNA of high-quality and adequate quantity. Current RNA purification approaches include phenol-chloroform extraction, affinity-based methods, and magnetic separation, leading to the separation of RNA from genomic deoxyribonucleic acid (DNA) and other cellular components (e.g., enzymes, salts, and nucleotides) after lysis of cells or tissues. Additionally, RNA transcribed in vitro needs to be separated from other components, including DNA templates, RNA-modifying enzymes and unincorporated nucleotides. Since centrifuge and column-based protocols commonly employed for the purification of RNA require specialized equipment and generally use toxic reagents, they are not easily scalable and adaptable for high-throughput analysis. This chapter provides an overview of RNA purification methods from various sources and highlights the versatility of purified RNA in basic research and biotechnological applications. © 2024 selection and editorial matter, Dongyou Liu; individual chapters. All rights reserved.
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    Sensor applications for detection in agricultural products, foods, and water
    (Springer International Publishing, 2022) Bakhshpour, Monireh; Göktürk, Ilgim; Gür, Sinem Diken; Yilmaz, Fatma; Denizli, Adil
    Pesticides are used to control pests that can destroy or reduce food production. Therefore, they are an important management tool to increase agricultural crop yield and reduce post-harvest losses, especially in a world facing hunger and famine. Although pesticides are useful in food production, they may have harmful effects such as being permanent in the environment and accumulating in living organisms, since they cannot be biodegradable. As pesticides have been widely used in agriculture for decades, concerns have been raised due to their known toxicity to human health. Thus, the determination of pesticides is of great importance due to both their increasing toxic effects on human health and the control of their use. Analytical technology methods developed using sensors are a wide-ranging field that affects industrial sectors such as medicine, health, food, agriculture, environment, and water. This chapter covers sensors and biosensors that have been developed in recent years to identify analytes sensitive to water pollution, especially used in food safety. Also, the basic principles of sensor systems used in the analysis of pollutants are mentioned. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.