RNA purification
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
CRC Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ribonucleic acid (RNA) molecules are used in various downstream experiments, including cloning, real-time reverse transcription polymerase chain reaction (RT-PCR), reverse transcription, and RNA-sequencing for gene expression analyses, which all require RNA of high-quality and adequate quantity. Current RNA purification approaches include phenol-chloroform extraction, affinity-based methods, and magnetic separation, leading to the separation of RNA from genomic deoxyribonucleic acid (DNA) and other cellular components (e.g., enzymes, salts, and nucleotides) after lysis of cells or tissues. Additionally, RNA transcribed in vitro needs to be separated from other components, including DNA templates, RNA-modifying enzymes and unincorporated nucleotides. Since centrifuge and column-based protocols commonly employed for the purification of RNA require specialized equipment and generally use toxic reagents, they are not easily scalable and adaptable for high-throughput analysis. This chapter provides an overview of RNA purification methods from various sources and highlights the versatility of purified RNA in basic research and biotechnological applications. © 2024 selection and editorial matter, Dongyou Liu; individual chapters. All rights reserved.
Açıklama
Anahtar Kelimeler
Kaynak
Handbook of Molecular Biotechnology
WoS Q Değeri
Scopus Q Değeri
N/A
