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  • Küçük Resim Yok
    Öğe
    Analysis of Antioxidant Effects of Lycopene and Caffeine in Experimentally Induced Renal Ischemia-Reperfusion Injury in Rats
    (2021) Saylan, Aslihan; Firat, Tülin; Yis, Özgür Mehmet; Dede, Gizem; Carver, Hande
    Background: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion (IR) injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were adminis- tered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and se- rum creatinine activities were measured biochemically and light microscopy findings were evaluated histo- logically. Results: When MDA levels are compared; Although the MDA level of the IR+Lycopene group was lower than the IR group, no significance was observed. When IR+Lycopene and IR+Caffeine groups are compared; The MDA level of the IR+Lycopene group is higher than the IR+ Caffeine group. In the IR+Caffeine group; A sta- tistically significant increase was observed in serum creatinine compared to the IR+Lycopene+Caffeine group. Although SOD and GSH-Px were higher in the antioxidant groups compared to the IR group, no sta- tistically significant difference was observed. When compared with ischemia-reperfusion group, histopatho- logical damage was found to be significantly lower than in the other groups. It was found that Lycopene and Caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, des- quame epithelium and proteinous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.
  • Küçük Resim Yok
    Öğe
    Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model
    (2013) Hapa, Onur; Çakici, Hüsamettin; Yüksel, Halil Yalçın; Firat, Tülin; Kükner, Aysel; Aygün, Hayati
    Amaç: Mevcut çalışmanın amacı trombositten zengin plazma (platelet-rich plasma, PRP) + mikrofraktür ile mikrofraktür tedavilerinin kronik fokal kondral defekt iyileşmesine etkisini karşılaştırmaktı. Çalışma planı: Bu çalışmada 57 adet erişkin erkek Sprague-Dawley sıçanı kullanıldı. Kırk iki sıçan kondral defekt oluşturulduktan sonra 14’erlikten 3 gruba (kontrol, sadece mikrofraktür, PRP+mikrofraktür) ayrıldı. Kalan 15 sıçan PRP hazırlanmasında kullanıldı. Tedaviden 3 ve 6 hafta sonra sıçanlara ötenazi uygulandı ve incelemeleri yapıldı. Uyarlanmış Pineda skorlama sistemi ile histolojik analiz ve Tip 2 kollajen için immünohistokimyasal boyama yapıldı. Bulgular: Her iki zaman aralığında da, kontrol grubu histolojik skorları (3. hafta: 8.8±1.2, 6. hafta: 8.5±0.7) mikrofraktür (3. hafta: 6.8±1.0, 6. hafta: 7.1±0.6) ve PRP+mikrofraktür (3. hafta: 6.4±1.3, 6. hafta: 5.7±1.2) gruplarından daha yüksekti (p<0.05). Altıncı hafta mikrofraktür grup skoru, 6. hafta PRP+mikrofraktür grubundan daha yüksekti (p<0.05). Tip 2 kollajen boyanma derecesi 6. haftada PRP+mikrofraktür grubunda daha yüksekti ve hücre membranında membranöz boyama gözlendiğinden benzersizdi. Çıkarımlar: Kronik fokal kondral defekt tedavisi için mikrofraktürlerle birlikte PRP uygulamasının kıkırdak iyileşmesini daha etkin kıldığı gözükmektedir.
  • Küçük Resim Yok
    Öğe
    Effects of adipose tissue-derived stromal vascular fraction on osteochondral defects treated by hyaluronic acid-based scaffold: An experimental study
    (2021) Şahin, Abdullah Alper; Değirmenci, Erdem; Özturan, Kutay Engin; Firat, Tülin; Kükner, Aysel
    Objectives: This study aims to evaluate the effect ofadipose-derived stromal vascular fraction (SVF) onosteochondral defects treated by hyaluronic acid (HA)-basedscaffold in a rabbit model.Materials and methods: Eighteen white New Zealand rabbitswere randomly grouped into the experimental group (n=9) andcontrol group (n=9). In all groups, osteochondral defects wereinduced on the weight-bearing surfaces of the right femoralmedial condyles, and a HA-based scaffold was applied to thedefect area with microfractures (MFs). In this study, 1 mLof adipose-derived SVF was injected into the knee joints ofthe rabbits in the experimental group. For histological andmacroscopic evaluation, four rabbits were randomly selectedfrom each group at Week 4, and the remaining rabbits weresacrificed at the end of Week 8. Macroscopic assessments of allsamples were performed based on the Brittberg scoring system,and microscopic evaluations were performed based on theO’Driscoll scores.Results: Samples were taken at Weeks 4 and 8. At Week 4,the O’Driscoll scores were significantly higher in the controlgroup than the experimental group (p=0.038), while there wasno significant difference in the Brittberg scores between the twogroups (p=0.108). At Week 8, the O’Driscoll score and Brittbergscores were statistically higher in the experimental groupthan in the control group (p=0.008 and p=0.007, respectively).According to the microscopic evaluation, at the end of Week 8,the cartilage thickness was greater in the experimental group,and nearly all of the defect area was filled with hyaline cartilage.Conclusion: Application of adipose-derived SVF withMF-HA-based scaffold was better than MF-HA-based scaffoldtreatment in improving osteochondral regeneration. Therefore,it can be used in combination with microfracture and scaffold toaccelerate cartilage regeneration, particularly in the treatment ofsecondary osteoarthritis.