Effects of adipose tissue-derived stromal vascular fraction on osteochondral defects treated by hyaluronic acid-based scaffold: An experimental study
Küçük Resim Yok
Tarih
2021
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Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: This study aims to evaluate the effect ofadipose-derived stromal vascular fraction (SVF) onosteochondral defects treated by hyaluronic acid (HA)-basedscaffold in a rabbit model.Materials and methods: Eighteen white New Zealand rabbitswere randomly grouped into the experimental group (n=9) andcontrol group (n=9). In all groups, osteochondral defects wereinduced on the weight-bearing surfaces of the right femoralmedial condyles, and a HA-based scaffold was applied to thedefect area with microfractures (MFs). In this study, 1 mLof adipose-derived SVF was injected into the knee joints ofthe rabbits in the experimental group. For histological andmacroscopic evaluation, four rabbits were randomly selectedfrom each group at Week 4, and the remaining rabbits weresacrificed at the end of Week 8. Macroscopic assessments of allsamples were performed based on the Brittberg scoring system,and microscopic evaluations were performed based on theO’Driscoll scores.Results: Samples were taken at Weeks 4 and 8. At Week 4,the O’Driscoll scores were significantly higher in the controlgroup than the experimental group (p=0.038), while there wasno significant difference in the Brittberg scores between the twogroups (p=0.108). At Week 8, the O’Driscoll score and Brittbergscores were statistically higher in the experimental groupthan in the control group (p=0.008 and p=0.007, respectively).According to the microscopic evaluation, at the end of Week 8,the cartilage thickness was greater in the experimental group,and nearly all of the defect area was filled with hyaline cartilage.Conclusion: Application of adipose-derived SVF withMF-HA-based scaffold was better than MF-HA-based scaffoldtreatment in improving osteochondral regeneration. Therefore,it can be used in combination with microfracture and scaffold toaccelerate cartilage regeneration, particularly in the treatment ofsecondary osteoarthritis.
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Anahtar Kelimeler
Kaynak
Joint diseases and related surgery
WoS Q Değeri
Scopus Q Değeri
Cilt
32
Sayı
2
