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Öğe Abelmoschus esculentus (L.) Moench seed extract alleviates acute acetaminophen induced liver damage in rats(National Institute of Science Communication and Information Resources (NISCAIR), 2023) Kocabey, Hüseyin; Bekdaş, Mervan; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Alışık, Murat; Türel, İdrisAcetaminophen (N-acetyl-p-aminophenol, APAP), commonly called the 'paracetamol', is one of the most regularly utilized medicines, particularly in children. When administered at the recommended doses, it is a safe medication. However, oxidative stress and inflammation caused by exposure to toxic doses lead to centrilobular hepatic necrosis. N-acetylcysteine (NAC) is utilized in the therapy, however it has potential adverse effects. On the other hand, it is known that the seeds of the common vegetable Okra, Abelmoschus esculentus (AE), a herbal product, possess antioxidant and anti-inflammatory qualities. In the present study, we explored whether AE can be used as an alternative to standard NAC therapy without any adverse effect in the treatment of acute APAP induced liver injury. Forty male Wistar rats were placed into five groups: Control, AE, APAP, APAP+AE and APAP+AE+NAC groups. Antioxidants such as native thiol and total thiol were found rised in the APAP group by adding AE (p =0.043 and p =0.028, respectively). Anti-inflammatory indicator IL-10 was also found increased, while marker ALT, which is a sign of hepatotoxicity, got decreased (P=0.005 and P <0.001, respectively). Histologically, AE has been shown to improve worsened congestion (P =0.003), cytoplasmic vacuolization (P =0.01), sinusoidal dilatation (P =0.001), Kupffer cell proliferation (P <0.001) and inflammation (P <0.001). These results suggest that the okra seeds may be a potential therapeutic agent for paracetamol induced hepatotoxicity and it can be attributed to the antioxidant and anti-inflammatory properties.Öğe Acinetobacter baumannii pneumonia increases surfactant proteins SP- A, SP-B, and SP-D levels, while decreasing SP-C level in bronchoalveolar lavage in rats(ELSEVIER, 2023) Özarslan, Talat Oğulcan; Sırmatel, Fatma; Karabörk, Şeyda Özsoy; Düzcü, Selma Erdoğan; Astarcı, Hesna MüzeyyenAcinetobacter baumannii is an opportunistic pathogen which causes nosocomial infections and high mortality. It is one of the most isolated microorganisms from patients with nosocomial pneumonia. Lung surfactant proteins (SP) are crucial for lung functions, and levels of SP-A, SP-B, SP-C, and SP-D change in the presence of infection. How they are affected in A. baumannii pneumonia, however, has not been conclusively researched. Therefore, the aim of the present study is to investigate the effect of A. baumannii pneumonia on surfactant proteins. 54 six-to-seven-month-old Sprague-Dawley rats were divided into two groups: sham and A. baumannii. The rats were administered 100 ml 0.5 McFarland A. baumannii or sterile saline intratracheally according to their group. Following the inoculation, lung and bronchoalveolar lavage fluids (BALF) were collected on the 3rd, 24th, and 48th hours of instillation. The lungs were examined histopathologically and the SP-A, SP-B, SP-C, and SP-D levels in BALF were measured with ELISA. The rats challenged with A. baumannii had significantly higher SP-A, SP-B, and SPD levels while having a significantly lower SP-C level compared to their sham equivalents. Furthermore, a strong positive correlation between SP-A and SP-D, and a moderate negative correlation between SP-B and SP-C were observed.Öğe Activation of TRESK background potassium channels by cloxyquin exerts protective effects against excitotoxic-induced brain injury and neuroinflammation in neonatal rats(Elseiver, 2022) Dilek, Mustafa; Kılınç, Yasemin Baranoğlu; Kılınç, Erkan; Torun, İbrahim Ethem; Saylan, Aslıhan; Düzcü, Selma ErdoğanWe investigated effects of activation of TRESK channels by selective activator cloxyquin on excitotoxic-induced brain injury and neuroinflammation involving brain mast cells and inflammatory cytokines in neonatal rats. Three different doses of cloxyquin (0.2, 1 and 5 mg/kg) were studied in ibotenate-induced perinatal brain injury (PBI) in P5 rat-pups. Cerebral lesions and mast cells in coronal brain sections were evaluated. Concentrations of activin A, IL-1 beta, IL-6 and IL-10 in brain homogenates were measured using ELISA. Cloxyquin dose-dependently exerted protective effects against excitotoxic-induced neonatal brain injury and neuroinflammation. TRESK channels may be a promising new target for the treatment of PBIs.Öğe Adrenal Myelolipom: Adrenal Bezin Nadir Bir Tümörü(2022) Karabekiroğlu, Nur Tunç; Düzcü, Selma Erdoğan; Gucuk, AdnanMyelolipomlar hematopoetik hücreler ile iç içe matür adipöz dokudan oluşan adrenal bezin nadir görülen benign ve hormonal olarak inaktif tümörleridir. Adrenal kortikal tümörler içerisinde ikinci sıklıkta görülürler. Bu tümörler genellikle görüntüleme işlemleri sırasında insidental olarak saptanırlar. Otopsi serilerinde insidansı yaklaşık olarak %0.08-0.2 arasında değişmektedir. Patogenezleri belirsiz olmakla birlikte primer olayın enfeksiyon, kronik stres veya adrenal bez dejenerasyonuna bağlı retiküloendotelyal hücrelerde meydana gelen metaplastik değişiklikler olduğu düşünülmektedir. Genellikle tek taraflı adrenal kitle olarak görülürler ve bu tümörlerin büyük kısmı asemptomatiktir. Nadiren 4 cm’den büyük kitle yapısı oluştururlar. Bu tümörlerde malign transformasyon bildirilmemiştir. Ancak benign tümörler olmalarına rağmen diğer adrenal tümörlerden ayırıcı tanısının yapılması önemlidir. Burada karın ağrısı ve kabızlık şikayetleriyle başvuran 40 yaşında erkek hastada yapılan tetkikler sırasında saptanan sağ adrenal bez yerleşimli myelolipom olgusu sunulmuştur ve myelolipomların klinikopatolojik özellikleri gözden geçirilmiştir.Öğe Adult-type granulosa cell tumor with splenic metastasis: a rare case(Cukurova Univ, Fac Medicine, 2020) Düzcü, Selma Erdoğan; Tunç, Nur; Boran, ÇetinTo the Editor, Granulosa cell tumors are rare malignant tumors defined by Rokitansky in 1855 and consist 2-3% of all ovarian tumors1. Incidence rate is 0.5-1.5/100.000 cases per year2. Characteristically it grows very slowly yet metastasizes years later after the curative tratment 1. The mean age is 50 years but it can be seen at any age after the menopause and patients admit to the clinics with complaints of uterine bleeding, pain and symptoms secondary to pressure2,3. A case with splenic metastasis after three years of the diagnosis of adult-type granulosa cell tumoÖğe Anaplastic thyroid carcinoma case series(2024) Küçüköztaş, Nadire; Duman, Tuba Taslamacioğlu; Düzcü, Selma Erdoğan; Rahatli, Samed; Üyetürk, ÜmmügülAim: Among the endocrine malignancies, thyroid carcinoma (TC) is the most common. However, anaplastic TC accounts for 1-2% of these cancers. The aim of this study was to evaluate the demographic and pathologic features, treatments, and survival of patients with anaplastic TC. Methods: Anaplastic TC patients who applied to our medical oncology clinics between 01.01.2012 -01.12.2018 were retrospectively evaluated. Results: A total of 8 patients were included in the study. There were 4 female and 4 male patients with a median age of 68 (minimum 61-maximum 83) years. The initial complaint of all patients was a fast-growing swelling in the neck. Six patients had total thyroidectomy. Two patients had anaplastic TC with a differential TC. Six patients were at stage 4C. The most common site of metastasis was the lung (75%). Five patients had received a median of 3 (1-6) cycles of chemotherapy. Radiotherapy was applied to 7 patients. All patients except one died during the follow-up period. The median survival time of the patients was 3 (2-15) months. Conclusion: Anaplastic TC, an aggressive tumor with high metastasic potential, has no effective treatment at present. Effective treatments are needed for this rare and aggressive disease. Developments in the molecular field are promising for the treatment of ATC.Öğe Benign macrocystic serous cystadenoma of the pancreas(Wolters Kluwer Medknow Publications, 2021) Düzcü, Selma Erdoğan; Tunç, NurSerous cystadenoma is a rare benign cystic lesion of pancreas. They are mostly known as benign cystic tumors of pancreas but malign transformation as serous cystadenocarcinoma is also reported. It is more commonly observed in women with the mean age of onset is 62 years. The majority of patients present nonspecific symptoms such as abdominal pain, weight loss, nausea, vomiting, fever, and melena. One-third of the patients are asymptomatic. A 60-year-old woman presents with abdominal pain and nausea for 1 month was admitted. Physical and laboratory findings were normal. Abdomen computed tomography scan confirmed a large number of millimetric cysts of 45 x 47 x 50 mm in size at the head of the pancreas. Due to patient's symptoms and mass effect, Whipple procedure was performed. In the gross examination, a nodular area of 5 x 5 x 4 cm was observed in the head of the pancreas. The microscopic examination of the material revealed cystic structures with fibrous stroma dotted with single layered cuboidal epithelium in the pancreatic tissue. The pathology report confirmed benign macrocystic serous cystadenoma. Serous cystadenomas are rare benign cystic lesions of the pancreas. Although they are benign lesions, it is crucial to differentiate them from other cystic lesions of the pancreas and malignant serous cystadenocarcinomas.Öğe Capsaicin attenuates excitotoxic-induced neonatal brain injury and brain mast cell-mediated neuroinflammation in newborn rats(Elsevier Ireland Ltd, 2023) Kılınç, Yasemin Baranoğlu; Dilek, Mustafa; Kılınç, Erkan; Torun, İbrahim Ethem; Şaylan, Aslıhan; Düzcü, Selma ErdoğanExcitotoxicity and neuroinflammation are key contributors to perinatal brain injuries. Capsaicin, an active ingredient of chili peppers, is a potent exogenous agonist for transient receptor potential vanilloid 1 receptors. Although the neuroprotective and anti-inflammatory effects of capsaicin are well-documented, its effects on excitotoxic-induced neonatal brain injury and neuroinflammation have not previously been investigated. The aim of this study was to investigate the effects of capsaicin on brain damage, brain mast cells, and inflammatory mediators in a model of ibotenate-induced excitotoxic brain injury in neonatal rats. P5 rat-pups were intraperitoneally injected with vehicle, 0.2-, 1-, and 5-mg/kg doses of capsaicin, or the NMDA (N-methyl-D-aspartate) receptor antagonist MK-801 (dizocilpine), 30 min before intracerebral injection of 10 mu g ibotenate. The naive-control group received no substance administration. The rat pups were sacrificed one or five days after ibotenate injection. Levels of activin A and interleukin (IL)-1 beta, IL-6, and IL-10 in brain tissue were measured using the enzyme-linked immunosorbent assay method. Cortex and white matter thicknesses, white matter lesion size, and mast cells were evaluated in brain sections stained with cresyl-violet or toluidineblue.Capsaicin improved ibotenate-induced white matter lesions and cerebral white and gray matter thicknesses in a dose-dependent manner. In addition, it suppressed the degranulation and increased number of brain mast cells induced by ibotenate. Capsaicin also reduced the excitotoxic-induced production of neuronal survival factor activin A and of the pro-inflammatory cytokines IL-1 beta, and IL-6 in brain tissue. However, IL-10 levels were not altered by the treatments. MK-801, as a positive control, reversed all these ibotenate-induced changes, further confirming the success of the model. Our findings provide, for the first time, evidence for the therapeutic effects of capsaicin against excitotoxicinduced neonatal brain injury and brain mast cell-mediated neuroinflammation. Capsaicin may therefore be a promising candidate in the prevention and/or reduction of neonatal brain damage.Öğe Carvacrol attenuates histopathogic and functional impairments induced by bilateral renal ischemia/reperfusion in rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2018) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Tekçe, Buket Kın; Öztürk, HayrettinBackground: Renal ischemia/reperfusion injury is the most common cause of acute kidney injury, which frequent occurrence in critically ill patients. Objectives: The aim of this study was to investigate the role of Carvacrol (CARV) against bilateral ischemia reperfusion (I/R) in rats. Methods: Renal I/R injury were induced by clamping of the left and right renal arteries for 45 min followed by 24 h of reperfusion. Thirty male Sprague-Dawley rats were randomly allocated to three groups (n = 10): the sham-control group, the renal I/R-untreated (I/R-untreated) group, and the I/R- CARV-treated group. At 2 h before reperfusion, the rats in the I/R- CARV - treated group rats received an i.p. injection of 75 mg/kg CARV. Renal function and histological changes were compared and the relevant parameters of oxidative stress and inflammation were detected. Results: Compared to the sham-control group, I/R led to renal dysfunction as evidenced by higher plasma urea and creatinine along with increase in oxidative stress and histological changes in renal tissues. Treatment with CARV decreased urea, creatinine, and renal MDA and MPO levels, and increased SOD, CAT, GSH activity and eNOS expression in the kidney. In the I/R-CARV-treated group, minimal hydropic changes in the tubular epithelial cells and regeneration of tubular epithelium were observed. Conclusion: These results suggest that CARV treatment could reduce renal injury induced by bilateral renal I/R via anti-inflammatory, antioxidant and cytoprotective effects.Öğe Carvacrol reduces the severity of intestinal mucosal damage caused by intestinal ischemia - Reperfusion in rats(Soc Stiinte Farmaceutice Romania, 2019) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Yis, Özgür Mehmet; Öztürk, HayrettinIntestinal ischemia-reperfusion (I/R) is a pathophysiological process that is common in many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation. Our aim in this study was to investigate the potential protective effects of carvacrol on the intestinal I/R injury in a rat model. The 30 rats were randomly divided into three groups (n = 10): The sham-control (group 1) underwent only the separation of the superior mesenteric artery but not the occlusion. In the FR-untreated (group 2) and I/R-carvacrol-treated groups (group 3), the superior mesenteric artery was clamped for 45 min, followed by 60 min of reperfusion. 2 hours before ischemia, the group 3 of rats received an intraperitoneal injection of carvacrol at a dose of 75 mg/kg bw. At the end of the experiment, intestinal tissue samples were taken for oxidative stress assessment including superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant status (TAS) and total oxidant status (TOS). In addition, the intestine sections were stained with haematoxylin-eosin to evaluate morphological changes and immunohistochemical staining was performed for inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) assessment. The intestinal mucosa was significantly damaged in the group 2, which was markedly attenuated after carvacrol treatment. The tissue MDA, MPO and TOS content increased significantly in the group 2, but they were reduced by carvacrol treatment. In addition, SOD and TAS activity increased markedly in group 3 as compared to group 2. Immunohistochemical staining showed that iNOS increased and eNOS decreased in group 2, which was improved in reverse direction after carvacrol treatment. Carvacrol may be a potential therapeutic agent for the treatment of intestinal I/R injury.Öğe Combination of novel c.3484g > t/p.glu162ter variant in abcb11 and c.208g > a/p.asp70asn variant in atp8b1 are associated with severe symptoms in progressive family ıntrahepatic cholestasis(Georg Thieme Verlag Kg, 2020) Bekdaş, Mervan; Can, Güray; Eröz, Recep; Düzcü, Selma ErdoğanProgressive family intrahepatic cholestasis (PFIC) is an autosomal recessive disease that causes chronic cholestasis. It is associated with pathogenic variants in genes that encode proteins involved in bile secretion to canaliculus from hepatocytes. In this study, we present a 16-year-old boy who presented with severe pruritus and cholestatic jaundice. All possible infectious etiologies were negative. A liver biopsy was consistent with intrahepatic cholestasis and portal fibrosis. DNA was isolated from a peripheral blood sample, and whole exome sequencing was performed. A novel c.3484G > T/p.Glu162Ter variant in theABCB11gene and a c.208G> A/p.Asp70Asn variant in theATP8B1gene were detected. Despite traditional treatment, the patient's recurrent severe symptoms did not improve. The patient was referred for a liver transplantation. This novel c.3484G > T/p.Glu162Ter variant is associated with a severe and recurrent presentation, and the two compound variants could explain the severity of PFIC.Öğe Comparison of modified scarff-bloom-richardson grading system with p16 and bcl-2 expression in invasive ductal carcinoma(Galenos Yayıncılık, 2015) Düzcü, Selma Erdoğan; Gürbüzel, Mihriban; Barut, Saime GülAim: Breast cancer is the most frequent cancer among women. The p16INK4a and bcl-2 genes are the two major pathways involved in control of the cell cycle. They also play key roles in breast carcinogenesis. In this study, we investigated the relationships between the histopathologic parameters in the "Modified Scarff-Bloom-Richardson" microscopic grading and expression of p16 and bcl-2 gene in cases with invasive ductal carcinoma. Methods: We examined 88 patients who were diagnosed with invasive ductal carcinoma between 2011 and 2013 in Haseki Educational and Research Hospital Pathology Department. Immunohistochemical staining for p16 and bcl-2 was performed. Results: Increased expression of p16 gene was statistically significantly related with increased tubule formation, pleomorfism, mitosis rate and histological grade (p=0.008, p=0.001, p=0.005, and p<0.001, respectively). We found a statistically significant relationship of the rise of the expression of bcl-2 gene with decreased mitosis rate and histological grade (p=0.026 and p=0.024, respectively). Conclusion: As a result, it was observed that there was a positive correlation between parameters included in Modified Scarff-Bloom- Richardson grading system with p16 in addition to negative correlation with bcl-2 expression. p16 and bcl-2 are important prognostic biomarkers in human breast cancer.Öğe Dermatofibrosarcoma protuberans: A rare malignancy(2017) Üyetürk, Ümmügül; Duman, Tuba Taslamacıoğlu; Düzcü, Selma Erdoğan; Küçüköztaş, Nadire; Yılmaz, FahriOBJECTIVE Dermatofibrosarcoma protuberans (DFSP) is a fibrohistiocytic tumor characterized by protuberant growth pattern of skin and subcutaneous tissue. The annual incidence is 4.2 per million. It is often seen between the ages of 20–50 years. This study aimed to evaluate the characteristics of patients with DFSP. METHODS Patients who visited our clinic were retrospectively evaluated. RESULTS All patients were females and the median age was 42 (29–60) years. The evaluated tumor sites were observed in the body/trunk in one patient, at the hip in two, and at the proximal lower extremity in two. Sur gical excision was done in all of them as initial treatment. Re-excision was needed in three patients. One of the patients underwent punch biopsy for diagnosis and surgical removal of the tumor with a negative surgical margin. One of the patients underwent radiotherapy. The median follow-up time was 10 (6–26) months. During the median follow-up, there was no local recurrence or metastasis. CONCLUSION As initial treatment, it is important to surgically remove tumors that had no distant metastasis. Positive or near surgical margins should be resected again. If a negative surgical margin cannot be achieved, radiotherapy should be used as the treatment. Imatinib mesylate is used for treatment in unresectable, recurrent, and/or metastatic tumors.Öğe Dermatofibrosarcoma protuberans: a rare malignancy(Kare Publ, 2017) Üyetürk, Ümmügül; Duman, Tuba Taslamacıoğlu; Düzcü, Selma Erdoğan; Küçüköztaş, Nadire; Yılmaz, FahriOBJECTIVE Dermatofibrosarcoma protuberans (DFSP) is a fibrohistiocytic tumor characterized by protuberant growth pattern of skin and subcutaneous tissue. The annual incidence is 4.2 per million. It is often seen between the ages of 20-50 years. This study aimed to evaluate the characteristics of patients with DFSP. METHODS Patients who visited our clinic were retrospectively evaluated. RESULTS All patients were females and the median age was 42 (29-60) years. The evaluated tumor sites were observed in the body/trunk in one patient, at the hip in two, and at the proximal lower extremity in two. Surgical excision was done in all of them as initial treatment. Re-excision was needed in three patients. One of the patients underwent punch biopsy for diagnosis and surgical removal of the tumor with a negative surgical margin. One of the patients underwent radiotherapy. The median follow-up time was 10 (6-26) months. During the median follow-up, there was no local recurrence or metastasis. CONCLUSION As initial treatment, it is important to surgically remove tumors that had no distant metastasis. Positive or near surgical margins should be resected again. If a negative surgical margin cannot be achieved, radiotherapy should be used as the treatment. Imatinib mesylate is used for treatment in unresectable, recurrent, and/or metastatic tumors.Öğe Diagnostic accuracy of fine-needle aspiration cytology of thyroid nodules and seven years of experience(Erciyes Univ Sch Medicine, 2023) Düzcü, Selma Erdoğan; Karabekiroğlu, Nur Tunç; Astarcı, Hesna Müzeyyen; Kayış, Seyit AliObjective: Thyroid fine-needle aspiration cytology is a dependable, effective method of detecting thyroid nodules. This study aims to assess the diagnostic distribution of thyroid fine-needle aspiration cytology, compare it to postoperative histological tissue diagnoses, and explore its diagnostic compatibility with published data.Materials and Methods: This single-center retrospective descriptive study included 172 cases diagnosed histopathologically out of the 1675 thyroid fine-needle aspiration cytology procedures performed in the Bolu Abant Izzet Baysal University Fac- ulty of Medicine, Department of Medical Pathology, between 2014 and 2020. The study examined the correlation between cytological and histopathological diagnoses.Results: Of the 172 cases, 132 were female (76.74%), and 40 were male (23.26%). The participants ranged in age from 14 to 79, with a mean age of 49.23 +/- 11.83. Fine-needle aspiration cytology results were compared to tissue diagnoses. The method's sensitivity was 61.29%, its specificity was 96.00%, its negative predictive value was 80.00%, its positive predictive value was 90.48%, and its diagnostic efficiency was 82.72%.Conclusion: In our study, the sensitivity and specificity values of cytology were high. Fine-needle aspiration cytology had limited diagnostic contributions in diagnosing papillary microcarcinoma and follicular lesions. In addition to increasing cy-topathology experience to improve diagnostic compliance, we believe evaluating patients with clinical and radiological find-ings will better benefit patient treatment.Öğe Disseminated eruptive granuloma annulare induced by levetiracetam(Taylor & Francis Ltd, 2017) Şereflican, Betül; Karapınar, Tekden; Düzcü, Selma Erdoğan; Türkoğlu, Şule AydınGranuloma annulare (GA) is a benign, granulomatous cutaneous disease without clear etiology. Disseminated and drug induced granuloma annulare is a rare presentation. We present a 47-year-old woman with diffuse circular erythematous eruptions following treatment with levetiracetam. Her clinical and histopathological findings were compatible with the diagnosis of granuloma annulare. To the best of our knowledge, there is no previous report of this skin disease as a result of levetiracetam use. We report this case to highlight this antiepileptic drug as a possible etiologic agent in disseminated granuloma annulare.Öğe Does methylene blue reduce adhesion during the healing process after tendon repair?(Turkish Joint Diseases Foundation, 2020) Boz, Mehmet; Çakıcı, Hüsamettin; Pakdil, Murat; Şahin, Abdullah Alper; Düzcü, Selma Erdoğan; Bala, Mehmet Murat; Çelik, MetinObjectives: This study aims to biomechanically and histopathologically investigate the effects of methylene blue (MB) on preventing postoperative adhesion in chickens undergoing full - thickness flexor tendon incision. Materials and methods: This study was performed between June 2017 and June 2018 on Hubbard JA 57 type chickens (age, 6 months; weight, 2.2?0.3 kg). Sixty-four tendons were studied in 32 chickens, including 16 in the control group and 16 in the experimental group. The second and third finger flexor digitorum profundus tendons of the left foot of each chicken were repaired primarily after a full -thickness incision approximately 1 cm proximal to the distal adhesion area. In the control (n=32) and experimental groups (n=32), 0.25 mL of normal saline and 0.25 mL of 1% MB solutions were applied locally to the surgical site, respectively. The operated limb was immobilized using a circular cast. 16 chickens were randomly selected in each group and examined at the fourth week, and the remaining 16 chickens were examined at the sixth week. Thirty-two of these tendons were evaluated using the Tang histopathological adhesion classification system, and the other 32 were evaluated with a biomechanical pull system. Results: Adhesion was found to be less in the experimental group compared to the control group at the end of the fourth and sixth weeks both histopathologically and biomechanically. Furthermore, adhesion was significantly less in the experimental group at the end of the sixth week compared to the fourth week both histopathologically and biomechanically. Conclusion: Histopathological and biomechanical results show that MB, which has anti-inflammatory, antiseptic, antimicrobial and antioxidant effects, reduces adhesion during the healing process after tendon repair. We think that local MB application, particularly in surgeries performed after this type of injury, will be beneficial on early rehabilitation and functional results.Öğe The effect of Anatolian syrup on experimentally induced acetaminophen and lipopolysaccharide associated acute kidney injury(2022) Düzcü, Selma Erdoğan; Çetinkaya, Ayhan; Efe, Muhammet; Kayiş, Seyit Ali; Bekdaş, Mervan; Yoldaş, Meyri Arzu; Tirink, Omer FarukBackground: Acute kidney injury develops as a result of various etiologies and pathological mechanisms, with a high mortality rate. This study aimed to investigate the efficacy of Anatolian syrup on histopathological variables in experimentally induced acetaminophen and lipopolysaccharide associated acute kidney injury. Methods: In this study, 5 groups were formed using 40 male Wistar albino rats (200- 220g, 2-4 months old), as follows: Sham, acetaminophen (1 g/kg intraperitoneal (ip)), 3) lipopolysaccharide (5 mg/kg ip), acetaminophen (1 g/kg ip) + Anatolian syrup (15 days orally), and lipopolysaccharide (5 mg/kg ip) + Anatolian syrup (15 days orally).Tubular atrophy, tubular dilatation, cytoplasmic vacuolization in tubular epithelial cells, tubular epithelial cell necrosis, interstitial inflammation, congestion, hemorrhage, glomerular damage and loss of brushy border were evaluated histopathologically semiquantitatively using scoring from 0 to 5. Results: In histopathological variables, tubular atrophy, tubular dilatation, cytoplasmic vacuolization, necrosis, congestion, hemorrhage, glomerular damage, and loss of brushy border were significantly reduced in the acetaminophen + Anatolian syrup group compared to the acetaminophen group and in the lipopolysaccharide + Anatolian syrup group compared to the lipopolysaccharide group (p<0.001). Conclusions: The Anatolian syrup was shown to protect histopathological variables in kidney damage caused by acetaminophen and lipopolysaccharide.Öğe Effect of methylphenidate at different doses on new bone formation with rapid maxillary expansion: A micro-CT and histomorphometric study in rats(Wiley, 2022) Dursun, Saffet; Erhamza, T. Sezen; Önder, Mustafa Ercüment; Düzcü, Selma ErdoğanObjectives This study aimed to evaluate the effect of systemically administered methylphenidate hydrochloride (MPH) on new bone formation in premaxillary suture after rapid maxillary expansion (RME). Setting and Sample Population Thirty-three Wistar rats were divided into four groups: Group 1 (high dose, 30/60 mg/kg MPH), Group 2 (low dose, 4/10 mg/kg MPH), Group 3 (positive control) and Group 4 (negative control). Methods RME was applied on the 70th day of the study. A 5-day RME period was followed by a 12-day retention period. The experiment was terminated on the 87th day. Micro-CT for radiological evaluation, haematoxylin-eosin and Masson's trichrome staining methods were used for histomorphometric evaluation. Results Among experimental groups with RME, the lowest number of osteoblasts and capillaries in Group 1 (P < .05). New bone formation, fibrous callus formation, distal osteotomy line, proximal osteotomy union and cortex remodelling were observed to be lower in Group 1 and Group 2 than Group 3 (P < .05). There was a statistically significant difference between Group 4 and each of the other groups (P = .000) in the evaluation of the results for bone mineral density, bone volume, bone volume percentage, trabecular thickness and trabecular number. Conclusions MPH reduces cellular activity for new bone formation in suture in RME groups. Before performing rapid maxillary expansion in patients using MPH, the use of the drug should be postponed after a multidisciplinary decision process or clinical doses should be lowered.Öğe The Effect of Wet Cupping Therapy on Anxiety and Thiol/Disulfide Homeostasis in Premature Ovarian Failure: An Experimental Study(2022) Çelik, Humeyra; Çetinkaya, Ayhan; Bozat, Bihter Gökçe; Düzcü, Selma Erdoğan; Alişik, Murat; Yis, Özgür MehmetObjective: The effect of wet cupping therapy on anxiety\rand thiol/disulfide homeostasis was evaluated in rats with premature\rovarian failure (POF) induced by cyclophosphamide (CYC). Material\rand Methods: Twenty four Wistar Albino female rats were divided\rinto control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping\r(n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally\rto POF and POF+wet cupping groups. One week later, wet cupping\rtherapy was applied to wet cupping and POF+wet cupping groups for\r5 minutes. The next day, Open Field Test and Elevated Plus Maze Test\rwere applied to all experimental groups to evaluate anxiety. After sacrification,\rto assess pattern formation, endometrial thickness and ovarian\rreserve were measured with hematoxylin-eosin staining.\rThiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione\rlevels were measured in serum to measure oxidative stress. Results:\rAlthough there was no statistical significance, it was observed\rthat anxiety decreased in POF+wet cupping compared to POF group.\rThere was no change in thiol-disulfide values in POF+wet cupping\rcompared to POF group (p>0.05). Conclusion: Wet cupping therapy\rdid not reduce anxiety in the experimental POF model and did not shift\rthe balance to positive in thiol/disulfide homeostasis in favor of thiols.\rHowever, the obtained graphics, numerical data and high standard deviation\rsuggest that meaningful data could be obtained with more subjects.
