Yazar "Aslam, Noreen" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Analyses of promoters in Arabidopsis thaliana of 3 beta HSD and 5 beta POR genes isolated from digitalis species(Bolu Abant İzzet Baysal Üniversitesi, 2018) Aslam, Noreen; Gürel, EkremYüksükotu olarak bilinen Digitalis ferruginea, kardiyak glikozitlerin üretimi için kullanılmaktadır. Progesteron 5?-redüktaz (5?POR1 ve 5?POR2) ve 3?-hidroksisteroid dehidrojenaz (3?-HSD) Digitalis türlerinde kardenolidin biyosentez yolundaki önemli anahtar basamak genleridir. Bu çalışmada 3?-HSD, 5?POR1 ve 5? POR2 promotörleri Digitalis ferruginea'nın genomik DNA'sından İlk kez izole edilip, dizi analizi yapılmıştır. Promoterlerin sekans bilgileri, bu promoterlerin ışık tepkileriyle ilgili bol cis-elementlere sahip olduğunu ortaya çıkarmıştır. 5?POR1promoter spesifik cis elemanları sirkadiyen ve ısı şokudur. 5?POR2promoter spesifik düzenleyici elemanlar savunma, stres ve metil jasmonattır. Yara yanıt elementi, 3?-HSDpromoter'da spesifik olarak bulunmuştur. Çiçek dalma transformasyonu ile transform olmuş Arabidopsis bitkilerinde 3?-HSDpromoterÖğe Bioinformatics tools make plant functional genomics studies easy(Springer International Publishing, 2017) Sameeullah, Muhammad; Aslam, Noreen; Khan, Faheem Ahmed; Aasim, MuhammadIn the era of omics with the huge available data sets in field of genomics, transcriptomics, proteomics, metabolomics and phenomics need the quick access and efficiently use of the information to validate the functions for the particlular gene family or the gene of interest in the plant cell. Therefore, user-friendly bioinformatics tools are utmost important for the students, researchers, and scientists to carry out successful functional genomics experiments. Here we describe the free available online databases and tools that can be utilized without prior deep knowledge of bioinformatics. The access to genomics, transcriptomics, metabolomics, and phenomics tools will be beneficial for designing of intelligent experiments. Keywords Functional genomics Gene expression Comparative genomics Metabolomics Bioinformatics toolsÖğe CRISPR/Cas9-mediated immunity in plants against pathogens(Caister Academic Press, 2017) Sameeullah, Muhammad; Khan, Faheem Ahmed; Özer, Göksel; Aslam, Noreen; Gürel, Ekrem; Karadeniz, TuranGlobal crop production is highly threatened due to pathogen invasion. The huge quantity of pesticides application, although harmful to the environment and human health, is carried out to prevent the crop losses worldwide, every year. Therefore, understanding the molecular mechanisms of pathogenicity and plant resistance against pathogens is important. The resistance against pathogens is regulated by three important phytohormones, viz. salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). Here we review the possible role of CRISPR technology to understand the plant pathogenicity by mutating genes responsible for pathogen invasion or up-regulating the phytohormones genes or resistant genes. Thus hormone biosynthesis genes, receptor and feeding genes of pathogens could be important targets for modifications using CRISPR/Cas9 following multiplexing tool box strategy in order to edit multiple genes simultaneously to produce super plants. Here we put forward our idea that the genes would be either mutated in case of plant receptor protein targets of pathogens or up-regulation of resistant genes or hormone biosynthesis genes will be better choice for resistance against pathogens.Öğe Crispr/cas9-mediated immunity in plants against pathogens(Caister Academic Press, 2018) Sameeullah, Muhammad; Khan, Faheem Ahmed; Özer, Göksel; Aslam, Noreen; Gurel, Ekrem; Waheed, Mohammad Tahir; Karadeniz, TuranGlobal crop production is highly threatened due to pathogen invasion. The huge quantity of pesticides application, although harmful to the environment and human health, is carried out to prevent the crop losses worldwide, every year. Therefore, understanding the molecular mechanisms of path-ogenicity and plant resistance against pathogens is important. The resistance against pathogens is regulated by three important phytohormones, viz. salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). Here we review the possible role of CRISPR technology to understand the plant pathogenicity by mutating genes responsible for pathogen invasion or up-regulating the phytohormones genes or resistant genes. Thus hormone biosynthesis genes, receptor and feeding genes of pathogens could be important targets for modifications using CRISPR/Cas9 following multiplexing tool box strategy in order to edit multiple genes simultane-ously to produce super plants. Here we put forward our idea that the genes would be either mutated in case of plant receptor protein targets of pathogens or up-regulation of resistant genes or hormone bio-synthesis genes will be better choice for resistance against pathogens. © 2018, Caister Academic Press. All rights reserved.Öğe CRISPR/Cas9-mediated Immunity in Plants Against Pathogens(Mdpi, 2018) Sameeullah, Muhammad; Khan, Faheem Ahmed; Ozer, Goksel; Aslam, Noreen; Gurel, Ekrem; Waheed, Mohammad Tahir; Karadeniz, TuranGlobal crop production is highly threatened due to pathogen invasion. The huge quantity of pesticides application, although harmful to the environment and human health, is carried out to prevent the crop losses worldwide, every year. Therefore, understanding the molecular mechanisms of pathogenicity and plant resistance against pathogens is important. The resistance against pathogens is regulated by three important phytohormones, viz. salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). Here we review the possible role of CRISPR technology to understand the plant pathogenicity by mutating genes responsible for pathogen invasion or up-regulating the phytohormones genes or resistant genes. Thus hormone biosynthesis genes, receptor and feeding genes of pathogens could be important targets for modifications using CRISPR/Cas9 following multiplexing tool box strategy in order to edit multiple genes simultaneously to produce super plants. Here we put forward our idea that the genes would be either mutated in case of plant receptor protein targets of pathogens or up-regulation of resistant genes or hormone biosynthesis genes will be better choice for resistance against pathogens.Öğe In silico functional analyses of SWEETs reveal cues for their role in AMF symbiosis(Springer International Publishing, 2016) Sameeullah, Muhammad; Demiral, Tijen; Aslam, Noreen; Baloch, Faheem Shehzad; Gürel, EkremSWEETs are novel class of sugar effluxers, which have unique functional role in plant biology. Besides nectar production, freezing tolerance, and transport of hexoses across tonoplast and growth-supporting role of pathogens, these SWEETs could have potential role in establishing powerful symbiotic relationship at the root interface and also in feeding to arbuscular mycorrhizal fungi (AMF) symbionts. The microarray or transcriptome expression of SWEET genes from colonized roots revealed that out of 28 Medicago SWEETs, three genes (MtSWEET1b, MtSWEET3c, and MtSWEET12) were induced specifically due to AMF symbiosis. The root type specific expression of these three genes was also enhanced by AMF colonization in rice. The degree of expression of OsSWEET1b, OsSWEET3b, and OsSWEET12 was increased in colonized large lateral roots (LLRM) and crown roots (CRM), while OsSWEET3b and OsSWEET12 were induced in fine lateral roots (FLRM) and CRM, respectively. Promoter regions of these SWEETs represent critical motif elements (MYCS, PB1S, and PHR1) which play critical role in establishment of AMF symbiosis and phosphate starvation-induced responses, respectively. Taken together, these SWEETs have potential to be explored via functional genomics tools to understand feeding mechanisms to symbionts. © Springer International Publishing Switzerland 2016.Öğe Isolation of the 3 beta-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thaliana(Springer, 2022) Aslam, Noreen; Sameeullah, Muhammad; Yıldırım, Muhammet; Baloğlu, Mehmet Cengiz; Lossl, Andreas G.; Gürel, Ekrem; Yücesan, Bahtiyar BuharaBackground Although members of the SDR gene family (short chain dehydrogenase) are distributed in kingdom of life, they have diverse roles in stress tolerance mechanism or secondary metabolite biosynthesis. Nevertheless, their precise roles in gene expression or regulation under stress are yet to be understood. Methods As a case study, we isolated, sequenced and functionally characterized the 3 beta-HSD promoter from Digitalis ferruginea subsp. ferruginea in Arabidopsis thaliana. Results The promoter fragment contained light and stress response elements such as Box-4, G-Box, TCT-motif, LAMP element, ABRE, ARE, WUN-motif, MYB, MYC, W box, STRE and Box S. The functional analysis of the 3 beta-HSD promoter in transgenic Arabidopsis seedlings showed that the promoter was expressed in cotyledon and root elongation zone in 2 days' seedlings. However, this expression was extended to hypocotyl and complete root in 6 days' seedlings. In 20 days-old seedlings, promoter expression was distributed to the whole seedling including hydathodes aperture, vascular bundle, shoot apical meristem, trichomes, midrib, leaf primordia, hypocotyl and xylem tissues. Further, expression of the promoter was enhanced or remained stable under the different abiotic stress conditions like osmotic, heat, cold, cadmium or low pH. In addition, the promoter also showed response to methyl jasmonate (MeJA) application. The expression could not be induced in wounded cotyledon most likely due to lack of interacting elements in the promoter fragment. Conclusions Taken together, the 3 beta-HSD promoter could be a candidate for the development of transgenic plants especially under changing environmental conditions.Öğe Plastidial expression of 3?-hydroxysteroid dehydrogenase and progesterone 5?-reductase genes confer enhanced salt tolerance in tobacco(MDPI, 2021) Sameeullah, Muhammad; Yıldırım, Muhammet; Aslam, Noreen; Baloğlu, Mehmet Cengiz; Yücesan, Buhara; Gürel, EkremThe short-chain dehydrogenase/reductase (SDR) gene family is widely distributed in all kingdoms of life. The SDR genes, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and progesterone 5-beta-reductases (P5 beta R1, P5 beta R2) play a crucial role in cardenolide biosynthesis pathway in the Digitalis species. However, their role in plant stress, especially in salinity stress management, remains unexplored. In the present study, transplastomic tobacco plants were developed by inserting the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes. The integration of transgenes in plastomes, copy number and transgene expression at transcript and protein level in transplastomic plants were confirmed by PCR, end-to-end PCR, qRT-PCR and Western blot analysis, respectively. Subcellular localization analysis showed that 3 beta-HSD and P5 beta R1 are cytoplasmic, and P5 beta R2 is tonoplast-localized. Transplastomic lines showed enhanced growth in terms of biomass and chlorophyll content compared to wild type (WT) under 300 mM salt stress. Under salt stress, transplastomic lines remained greener without negative impact on shoot or root growth compared to the WT. The salt-tolerant transplastomic lines exhibited enhanced levels of a series of metabolites (sucrose, glutamate, glutamine and proline) under control and NaCl stress. Furthermore, a lower Na+/K+ ratio in transplastomic lines was also observed. The salt tolerance, mediated by plastidial expression of the 3 beta-HSD, P5 beta R1 and P5 beta R2 genes, could be due to the involvement in the upregulation of nitrogen assimilation, osmolytes as well as lower Na+/K+ ratio. Taken together, the plastid-based expression of the SDR genes leading to enhanced salt tolerance, which opens a window for developing saline-tolerant plants via plastid genetic engineering.Öğe Shoot-tip cultivation and cardenolide content analysis of natural populations of Digitalis Lanata Ehrh. Subsp. Lanata (Wooly Foxglove) in Thrace Region(2018) Yücesan, Bahtiyar Buhara; Eker, İsmail; Lazzarini, Luiz Eduardo Santos; Aslam, Noreen; Mohammed, Aliyu; Gürel, EkremDigitalis lanata, due to the presence of cardenolides in the leaves, is widely used in drug industry for the treatments of congestive heart diesases. In this present study, natural populations of D. lanata in Thrace region were collected. Thereafter, cardiac glycoside analysis of D. lanata subsp. lanata grown in natural habitats and micropropagation from shoot tips were established as a model work for a consistent and a sustainable plant production. Of the cardenolides, Lan A (ranging between 24.8 and 300.4 mg 100g-1) and Lan C (42.1 and 258 mg 100 g-1 were predominantly found in the samples as compared to lanatoside B, digoxin and digitoxin. After seed germination, shoot-tip cultivation was achieved after a subsequent shoot and root formation on MS medium. Indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) depending on their concentrations were found to be effective in shoot and root formation accordingly. Maximum two shoots from shoot tips were produced after 8 weeks of cultivation, and at the end of 12th weeks of cultivation each shoots produced an average number of 6.4 roots in 8.1 cm length. Acclimatization was also achieved successfully with a maximum survival rate (95 %) for 4 weeks. This study is the first report on cardenolide profiling of Thracian D. lanata at different locations, and shoot tip cultivation of the population selected under in vitro conditions.
