Yazar "Çetinkaya, Ayhan" seçeneğine göre listele
Listeleniyor 1 - 20 / 65
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Abelmoschus esculentus (L.) Moench seed extract alleviates acute acetaminophen induced liver damage in rats(National Institute of Science Communication and Information Resources (NISCAIR), 2023) Kocabey, Hüseyin; Bekdaş, Mervan; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Alışık, Murat; Türel, İdrisAcetaminophen (N-acetyl-p-aminophenol, APAP), commonly called the 'paracetamol', is one of the most regularly utilized medicines, particularly in children. When administered at the recommended doses, it is a safe medication. However, oxidative stress and inflammation caused by exposure to toxic doses lead to centrilobular hepatic necrosis. N-acetylcysteine (NAC) is utilized in the therapy, however it has potential adverse effects. On the other hand, it is known that the seeds of the common vegetable Okra, Abelmoschus esculentus (AE), a herbal product, possess antioxidant and anti-inflammatory qualities. In the present study, we explored whether AE can be used as an alternative to standard NAC therapy without any adverse effect in the treatment of acute APAP induced liver injury. Forty male Wistar rats were placed into five groups: Control, AE, APAP, APAP+AE and APAP+AE+NAC groups. Antioxidants such as native thiol and total thiol were found rised in the APAP group by adding AE (p =0.043 and p =0.028, respectively). Anti-inflammatory indicator IL-10 was also found increased, while marker ALT, which is a sign of hepatotoxicity, got decreased (P=0.005 and P <0.001, respectively). Histologically, AE has been shown to improve worsened congestion (P =0.003), cytoplasmic vacuolization (P =0.01), sinusoidal dilatation (P =0.001), Kupffer cell proliferation (P <0.001) and inflammation (P <0.001). These results suggest that the okra seeds may be a potential therapeutic agent for paracetamol induced hepatotoxicity and it can be attributed to the antioxidant and anti-inflammatory properties.Öğe Alleviation of kidney damage induced by unilateral ureter obstruction in rats by rhodiola rosea(Mary Ann Liebert, Inc, 2013) Üyetürk, Uğur; Terzi, E. Hakan; Kemahlı, Eray; Gücük, Adnan; Tosun, Mehmet; Çetinkaya, AyhanPurpose: To evaluate the efficacy of Rhodiola rosea extract in terms of alleviating the renal damage induced by unilateral ureter obstruction (UUO) in rats. Material and Methods: Thirty Wistar albino male rats were divided into five groups: (I) Control, (II) UUO 7 days, (III) UUO 7 days+extract,(IV) UUO 14 days, and (V) UUO 14 days+extract. Seven or 14 days after the initiation of the experimental procedure, the left kidneys of rats in all five groups were removed for histological examination, and their blood was drawn for biochemical measurements. Result: Median malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were, respectively, 39.4 (5.04) nmol/mL and 25.8 (8.01) nmol/minute/mL in group I, 77.9 (12.38) nmol/mL and 5.8 (1.95) nmol/minute/mL in group II, 48.7 (12.1) nmol/mL and 9.1 (2.3) nmol/minute/mL in group III, 58.5 (23.83) nmol/mL and 8.4 (2.1) nmol/minute/mL in group IV, and 44.8 (4.97) nmol/mL and 13.8 (3.73) nmol/minute/mL in group V. There was a statistically significant difference among the groups in terms of MDA and GPx levels (p<0.05 for both). The median numbers of apoptotic cells were 1 (1), 8 (2.25), 3 (1.25), 23.5 (9), and 7 (I) in groups I, II, III, IV, and V, respectively. There was a statistically siginificant difference among the groups in terms of apoptotic cell number (p<0.05). Conclusion:R. rosea extract was shown to alleviate the renal damage induced by UUO through its antioxidant effects. The mechanism by which R. rosea extract causes these effects merits further investigation.Öğe Ameliorating effect of hawthorn (Crataegus oxyacantha) and physical exercise on acute penicillin induced seizures in gerbils(African Networks Ethnomedicines, 2016) Çakır, Serkan; Orallar, Hayriye; Çetinkaya, Ayhan; Kayacan, Yıldırım; Önal, Ali Can; Yıldırım, Arzu; Benek, Selim; Özkan, Murat; Okur, NezihBackground: The aim of the present study was to assess the effect of Hawthorn (Crataegus oxyacantha) and physical activity. We studied its effect on penicillin induced epilepsy. in gerbils. Materal and Methods: Epilepsy was induced by administration of peniciline G (500 IU, ip). The gerbils were divided randomly in four groups (6 animals per each group) and studied as described below: 1) Control group 2) Exercise group (30 min/each day for 8 weeks) (Eg) 3) Extract group, 50mg/kg/day/animal in 1 ml saline, 3 h prior to exercise (Exe) 4) Exercise+ Extract + (Exe+ Ex). The severity of epilepsy was observed and recorded. Results: The means of latencies (Mean +/- SE) were 236 +/- 45, 369 +/- 36, 386 +/- 58 and 433 +/- 37 ms in groups of control, Exe, Ex, and Exe+ Ex respectively. The mean spike latency significantly (P= 0,033 F= 3,560) decreased in Exe, Ex and Exe+ Ex when compared control. Although spike frequency significantly (P< 0.05) diminished in groups of Exe and Ex, no significant decrease was observed in control and Exe+ Ex. Similar trend was seen for amplitude values. Spike amplitude values were determined to be significantly (P< 0.05) lower than those of control and Exe+ Ex. Conclusion: Crataegus oxyacantha extract has shown positive affect to ameliorate on some seizure parameters in this study. However, further more advanced physiologic and neurochemical studies are required to determine the mechanisms involved.Öğe Anti-inflammatory potential of liraglutide, a glucagon-like peptide-1 receptor agonist, in rats with peripheral acute inflammation(Springer, 2022) Mert, İrem; Çetinkaya, Ayhan; Gürler, Müjgan; Türel, Canan Akünal; Çelik, Hümeyra; Torun, İbrahim Ethem; Türel, İdrisThe present study aimed to explore the possible anti-inflammatory actions of liraglutide (LRG), a glucagon-like peptide-1 (GLP-1) receptor agonist, and to compare with tramadol (TR) or LRG, and TR combination treatment by investigating the inflammatory signs such as pain hypersensitivity, edema, and fever in carrageenan (CG)-induced acute peripheral inflammation model in rats. The levels of several biomarkers for inflammatory status, angiogenesis, and oxidative stress were also measured in inflamed tissues. CG induced inflammation in the paws of rats identified by hypersensitivities, redness, edema and fever. LRG, significantly improved the hypersensitivity to mechanical (from 4 to 35.5 g) or cold (from 5 to 44.2 s) stimuli, reduced the edema (paw mass, from 2.54 to 1.85 g), and fever (paw temperature, from 33.6 to 27.3 degrees C). LRG dramatically suppressed the inflammatory signs when compared to those of TR. In addition, co-administration of TR and LRG resulted in further reduction of sensitivity to mechanical and cold stimuli. Anti-inflammatory potential of LRG altered depending on their inhibitory effects in the biomarkers of inflamed paws. Consequently, the suppressive actions of LRG in the inflammation induced hypersensitivities, edema, and fever, indicating that these drugs have significant anti-inflammatory potential with anti-hypersensitivities, anti-edema, and anti-pyretic effects. LRG with anti-inflammatory actions may be a highly promising therapeutic option for the management of inflammatory conditions or inflammatory-related various diseases.Öğe Anti-proliferative effects of salmon calcitonin on SH-SY5Y neuroblastoma in vitro(2023) Varol, Muhammed Ismail; Karabörk, Şeyda; Çetinkaya, AyhanAim: We aimed to examine the potential cytotoxic effect of salmon calcitonin, which is one of the components that regulates mineral metabolism and prevents the increase in the amount of calcium, on SH-SY5Y cells, a neuroblastoma cell line.Methods: SH-SY5Y cells were cultured in DMEM medium in the presence of 37°C and 5% CO2in conventional culture flasks. MTT assay was applied to investigate the effect of calcitonin individually on SH-SY5Y cells by treatment different concentrations for 24 h and performed. Results: In cells cultured with salmon calcitonin applied at different concentrations (0.1, 1, 3.125, 6,25, 12.5, 25, 50 and 100 nM/ml), anti-proliferation was statistically significant at concentrations of 50 and 100 nM/ml compared to the control group. It showed that 50 nM/ml and 100 nM/ml had the highest cytotoxic effect on SH-SY5Y for 24 hConclusions: Considering the proliferation curve of SH-SY5Y, the results show that salmon calcitonin treatment potentiated the proliferative activities by inhibiting cell viability in SH-SY5Y cells at concentrations of 50 and 100 nM/ml. Further studies exploring salmon calcitonin’s protective effects may prove successful and maybe it is a promising agent for cancer treatmenÖğe The antiinflammatory and electrophysiological effects of fingolimod on penicillin-induced rats(Associação Arquivos de Neuro-Psiquiatria, 2022) Türel, Canan Akünal; Çelik, Hümeyra; Torun, İbrahim Ethem; Çetinkaya, Ayhan; Türel, İdrisBackground The fact that inflammation triggers epileptic seizures brings to mind the antiepileptic properties of anti-inflammatory drugs.Objective To investigate the electrophysiological and anti-inflammatory effects of fingolimod on an experimental penicillin-induced acute epileptic seizure model in rats.Methods Thirty-two male Wistar rats were divided into four groups: control (penicillin), positive control (penicillin + diazepam [5 mg/kg]), drug (penicillin + fingolimod [0.3 mg/kg]) and synergy group (penicillin + diazepam + fingolimod). The animals were anesthetized with urethane, and epileptiform activity was induced by intracortical injection of penicillin (500,000 IU). After electrophysiological recording for 125 minutes, IL-1 beta, TNF-alpha, and IL-6 were evaluated by ELISA in the serum of sacrificed animals.Results During the experiment, animal deaths occurred in the synergy group due to the synergistic negative chronotropic effect of diazepam and fingolimod. Although not statistically significant, fingolimod caused a slight decrease in spike-wave activity and spike amplitudes in the acute seizure model induced by penicillin ( p > 0.05). Fingolimod decreased serum IL-1 beta ( p < 0.05); fingolimod and diazepam together reduced IL-6 ( p < 0.05), but no change was observed in serum TNF-alpha values.Conclusion Even in acute use, the spike-wave and amplitude values of fingolimod decrease with diazepam, anticonvulsant and anti-inflammatory effects of fingolimod will be more prominent in chronic applications and central tissue evaluations. In addition, concomitant use of fingolimod and diazepam is considered to be contraindicated due to the synergistic negative inotropic effect.Öğe Can excessive oxygen cause hyperactive behavior disorder in preterm children? Cognitive effects of hyperoxia in the preterm brain of rats(Springer, 2019) Dilek, Mustafa; Orallar, Hayriye; Çetinkaya, Ayhan; Bozat, Gökçe; Pehlivan, Fatma; Bekdaş, Mervan; Kabakuş, NimetThere is a paucity of data on the effects of hyperoxia-induced brain damage on learning and such psychosocial phenomenon as anxiety. Preterm infants encounter hyperoxia within a relatively early stage of life (leaving the intrauterine environment earlier than was expected) and are exposed to high-level hyperoxic stress due to the insufficiency of their antioxidant defense mechanisms. In an experimental rat model, we investigated the effects of early postnatal hyperoxia on learning, anxiety, and depression in the early adulthood period. Rat nestlings (n = 7) were exposed to about 80% oxygen for the first 5 days after birth to create a rat model of hyperoxia, and these nestlings and those of the control group (n = 7) were subjected to behavioral tests (Morris water tank, open-field test, elevated plus maze, and Porsolt test) at 30 days old. Video recordings of the tests were captured, and indices of the tests in the experimental groups were compared with the Mann-Whitney U-test. In the Morris water navigation task, the latency and distance required to locate the platform were greater (P = 0.018 and 0.025, respectively) in the hyperoxia group than in the control group, suggesting that exposure to hyperoxia during the development of the brain can exert a negative effect on the learning function. There was no difference in the time spent in the open center area of the open-field test (anxiety), while the rats in the hyperoxia group spent more time in the enclosed area in the elevated plus maze test, suggesting a higher level of anxiety (P = 0.048). In the Porsolt test, rats in the hyperoxia group moved faster (P = 0.013) and travelled a longer distance (P = 0.048). Although this finding suggests less depressive behavior in the mentioned group, which was contrary to the expectations, this may also explain the tendency of preterm infants to hyperactivity at later ages. Thus, it has been demonstrated experimentally that exposure of neonates to oxygen exceeding physiological needs may cause behavioral problems, such as impaired learning, anxiety, and hyperactivity.Öğe Carvacrol attenuates histopathogic and functional impairments induced by bilateral renal ischemia/reperfusion in rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2018) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Tekçe, Buket Kın; Öztürk, HayrettinBackground: Renal ischemia/reperfusion injury is the most common cause of acute kidney injury, which frequent occurrence in critically ill patients. Objectives: The aim of this study was to investigate the role of Carvacrol (CARV) against bilateral ischemia reperfusion (I/R) in rats. Methods: Renal I/R injury were induced by clamping of the left and right renal arteries for 45 min followed by 24 h of reperfusion. Thirty male Sprague-Dawley rats were randomly allocated to three groups (n = 10): the sham-control group, the renal I/R-untreated (I/R-untreated) group, and the I/R- CARV-treated group. At 2 h before reperfusion, the rats in the I/R- CARV - treated group rats received an i.p. injection of 75 mg/kg CARV. Renal function and histological changes were compared and the relevant parameters of oxidative stress and inflammation were detected. Results: Compared to the sham-control group, I/R led to renal dysfunction as evidenced by higher plasma urea and creatinine along with increase in oxidative stress and histological changes in renal tissues. Treatment with CARV decreased urea, creatinine, and renal MDA and MPO levels, and increased SOD, CAT, GSH activity and eNOS expression in the kidney. In the I/R-CARV-treated group, minimal hydropic changes in the tubular epithelial cells and regeneration of tubular epithelium were observed. Conclusion: These results suggest that CARV treatment could reduce renal injury induced by bilateral renal I/R via anti-inflammatory, antioxidant and cytoprotective effects.Öğe Carvacrol reduces the severity of intestinal mucosal damage caused by intestinal ischemia - Reperfusion in rats(Soc Stiinte Farmaceutice Romania, 2019) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Yis, Özgür Mehmet; Öztürk, HayrettinIntestinal ischemia-reperfusion (I/R) is a pathophysiological process that is common in many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation. Our aim in this study was to investigate the potential protective effects of carvacrol on the intestinal I/R injury in a rat model. The 30 rats were randomly divided into three groups (n = 10): The sham-control (group 1) underwent only the separation of the superior mesenteric artery but not the occlusion. In the FR-untreated (group 2) and I/R-carvacrol-treated groups (group 3), the superior mesenteric artery was clamped for 45 min, followed by 60 min of reperfusion. 2 hours before ischemia, the group 3 of rats received an intraperitoneal injection of carvacrol at a dose of 75 mg/kg bw. At the end of the experiment, intestinal tissue samples were taken for oxidative stress assessment including superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant status (TAS) and total oxidant status (TOS). In addition, the intestine sections were stained with haematoxylin-eosin to evaluate morphological changes and immunohistochemical staining was performed for inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) assessment. The intestinal mucosa was significantly damaged in the group 2, which was markedly attenuated after carvacrol treatment. The tissue MDA, MPO and TOS content increased significantly in the group 2, but they were reduced by carvacrol treatment. In addition, SOD and TAS activity increased markedly in group 3 as compared to group 2. Immunohistochemical staining showed that iNOS increased and eNOS decreased in group 2, which was improved in reverse direction after carvacrol treatment. Carvacrol may be a potential therapeutic agent for the treatment of intestinal I/R injury.Öğe Comparison of cerebral effects of thiopental and propofol infusion in traumatic brain injured rats(2022) Kiliccioğlu, Yıldıray; Yildiz, Isa; Yoldaş, Hamit; Karagoz, Ibrahim; Tekçe, Buket Kin; Çetinkaya, Ayhan; Bilgi, MuratObjective: Head trauma is a lethal, disabling, and pathological condition requiring long-term treatment and care. Thiopental and propofol infusions are frequently used for sedation in the intensive care unit (ICU). However, we do not have clear data on whether they are neuroprotective or neurotoxic. We aimed to compare the early cerebral effects of propofol and thiopental, which are used for sedation in acute head trauma. Methods: A total of 30 rats were included in this experimental study, and the animals were randomly divided into three groups; 5 ml/kg/h 0.9% dose NaCl infusion was given in the control group, 30 mg/kg/h dose propofol infusion was given in the propofol group, and 140 mcg/kg/h dose of thiopental infusion was given in the thiopental group. Blood samples were taken 4 hours after infusion. A craniotomy was performed, the brain was removed, and it was placed in 10% neutral formalin for histological examination. The materials were examined biochemically and histologically and then compared between the groups. Results: The S100B value between the groups was significantly lower in the thiopental group than in the control group (p=0.018). Tau protein levels were significantly lower in the propofol group than in the control group (p=0.07). In histological examinations, the number of apoptotic cells in the propofol and thiopental groups were significantly lower than in the control group (p=0.02). There was no significant difference between the propofol and thiopental groups in apoptotic cell numbers (p=0.3). Conclusion: Our study demonstrated that thiopental and propofol infusions following a head trauma reduced apoptotic cell death and caused a decrease in trauma markers.Öğe The Comparison of Effects of Applications of Compound 48/80 and Mast Cell Mediator Suspension on Inflammation in Rats: A Methodological Study for Acute Inflammatory Pain(2019) Kılınç, Erkan; Dağıstan, Yaşar; Çetinkaya, Ayhan; Töre, FatmaObjective: Inflammation underlies the pathological basis of most diseases. Substance-P is a key mediator that participates in various inflammatory processes and painful conditions. Mast cells (MCs) have a key role in inflammatory processes via mediators released from their granules. The experimental models for the investigation of pathogenesis and treatment of inflammatory diseases represent merely certain characteristics of inflammatory cases, therefore, more comprehensive models are required. We aimed to compare effects of administrations of the compound-48/80 and mast cell mediator suspension (MCMS) obtained from peritoneal MCs on the inflammation in rats. Methods: Rats were divided into five groups (n=6): Intraperitoneally, Control group received 0.2 ml saline; C-48/80 group received 2 mg/kg compound-48/80; MCMS group received 0.2 ml MCMS; Cr+C-48/80 group received 10 mg/kg cromolyn plus compound-48/80; Cr+MCMS group received cromolyn plus MCMS. Potent inflammatory markers, plasma substance-P levels, and number and degranulation of dural MCs were measured. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test. Results: Compound-48/80 increased plasma substance-P levels (p<0.05) and dural MC-degranulation (p<0.001). Likewise, MCMS increased substance-P levels and dural MC-degranulation (p<0.001) as well as number of dural MCs (p<0.01). MC stabilizer cromolyn inhibited increases in the parameters induced by compound-48/80 and MCMS (p<0.01 and p<0.05, respectively). Conclusion: MCMS administration had greater impact to increase the plasma substance-P levels and number and degranulation of dural MCs than that of the compound-48/80 administration. The results demonstrate the potent inflammatory effect of MCMS treatment over the compund-48/80 administration. Administration of MCMS could be a useful tool to study inflammatory conditions.Öğe The comparison of the effects of application of compound 48/80 and autologous mast cell mediator suspension on plasma substance-P levels and dural mast cells in rats: a methodological study for acute Inflammatory Pain(Wiley, 2017) Kılınç, Erkan; Dağıstan, Yaşar; Çetinkaya, Ayhan; Töre, Fatma[No Abstract Available]Öğe Comparison of the effects of topical corneal inhibitory agents on TTL and PON1 in rats(Rjpbcs Research Journal Pharmaceutical, Biological & Chemical Sciences, 2017) Soydan, Adem; Yazar, Hayrullah; Çetinkaya, Ayhan; Terzi, Elçin Hakan; Ulaş, Fatih; Doğan, ÜmitThe aim of this study was to compare the effects of topical corneal inhibitory agents on total thiol (TTL) and paraoxonase 1 (PON1) levels in rats with experimentally acquired keratoconjunctivitis. Thirty-five rats were divided into five groups. Twenty-four hours prior to the experiment, keratoconjunctivitis was established in the right eye of the rats using sodium hydroxide. The treatments of the five groups were as follows: group I: (control) isotonic saline (0.9%), group II: topical 0.05% cyclosporine A, group III: topical 1% diluted propolis, group IV: topical 3% diluted propolis, and group V: 0.1% dexamethasone. At the end of the 10th day, one rat in each group, except the cyclosporine group (group II), had died. The treatment was applied to all groups three times a day for 10 days. Subsequently, blood samples were obtained and used for determining the levels of TTL and PON1 (Architect C16000). All statistical analyses were performed using IBM SPSS for Windows Version 20.0 software. Descriptive statistics were calculated from the values obtained from this study and shown as arithmetic mean and standard deviation. Kruskal Wallis variance analysis was conducted. p values found to be under 0.05 were accepted as statistically significant. The study was performed after the approval [By The Animal Research Ethics Committee, Bolu Abant Izzet Baysal University, Number: 13.30.2.ABU.0.05.05-050.01.04-1, January.8.2016]. The TTL results were as follows (mu mol/L): group I: 253.24, group II: 238.70, group III: 281.39, group IV: 284.80 and group V: 260.65. No marked differences were observed between the control group and the other groups (P>0.05). The PON1 results were as follows (U/L): group I: 521.49, group II: 472.30, group III: 362.37, group IV: 327.48 and group V: 440.31. No marked difference was observed between the control group and the other groups (P > 0.05). However, there was a marked difference in the PON1 results between the 1% and 3% propolis groups. CNV inhibitor agents' effects on TTL and PON1 values were similar.Öğe Cytotoxic effects of calcitriol on osteosarcoma cells(2022) Çelik, Humeyra; Çetinkaya, Ayhan; Celik, IlhanIntroduction: It was aimed to examine the effect of calcitriol which has known anticancer activities, on the osteosarcoma cell line SAOS-2 cells [1,25(OH)2D3]. Methods: SAOS-2 cells were grown in culture in conventional culture flasks in DMEM medium at 37°C and 5% CO2. When the cells were 70-80% confluent, morphological changes were examined under an inverted microscope. The cells were passaged into 96 microplates, and after passage, different concentrations of calcitriol was applied to the cells (0.1; 0.5; 1; 5; 10; 25 nM/ml) was done. After administration, cytotoxic effect and proliferation rates/cell proliferation were analyzed by MTT method. Results: The effect of calcitriol applied at different concentrations in cultured cells were 0.1, 1, 10, 25 nM/ml doses, in these groups proliferation was found to be statistically significantly reduced compared to the control group (p?0.05). Conclusion: Our data imply that the antiproliferative effects of vitamin D applications can be benefited from in exosomal, combined and supportive treatments in osteosarcoma. Further trials are warranted to confirm and validate our findings.Öğe Development of venous thromboembolism in patients with hereditary thrombophilic risk factors and evaluation of genetic and biochemical parameters affecting development of venous thromboembolism(2020) Korkmaz, Ufuk Turan Kürşat; Çetinkaya, Ayhan; Yüksel, Ahmet; Velioğlu, Yusuf; Uçaroğlu, Erhan Renan; Arslan, Ali Osman; Erdem, KemalettinObjectives: To evaluate patients diagnosed with venous thromboembolism (VTE) in terms of hereditary thrombophilic risk factors and to assess genetic and biochemical factors affecting the development of VTE. Methods: Sixty patients with VTE and 23 control subjects without VTE were retrospectively evaluated. Prevalence of thrombophilic risk factors and parameters like demographic data, clinical follow-up duration were examined with genetic, biochemical and radiological investigations. Results: Mutations were detected in the genes of Factor V Leiden in 37.4%, Factor II in 13.4%, Methylenetetrahydrofolate reductase C677 in 47.5%, Methylenetetrahydrofolate reductase A1298C in 53.3%, Plasminogen activator inhibitor-1 in 31.6%, Angiotensin converting enzyme in 39.0%, and Factor V H1299R in 8.3% of patients. Protein C deficiency was detected in 25 patients (41.7%), free Protein S deficiency was detected in 25 patients (41.7%) in study group and 3 subjects (13.0%) in control group, and this difference was statistically significant (p=0.023). Antithrombin III deficiency was detected in 1 patient (1.7%) in study group. Elevated homocysteine was higher in study group compared to controls, and the difference was statistically significant (p=0.02). Conclusion: Determination of hereditary risk factors in VTE patients will provide family members who have hereditary risk factors, but did not suffer attack to be protected against thromboembolic attacks by taking simple measures against acquired factors.Öğe Different exercise intensity and oxidative stress responses: thiol/disulfide homeostasis(Wiley, 2018) Kayacan, Yıldırım; Çetinkaya, Ayhan; Yazar, Hayrullah; Makaracı, Yücel[No Abstract Available]Öğe Dynamic thiol/disulphide homeostasis metrics as a risk factor for peripheral arterial disease(Sage Publications Ltd, 2021) Korkmaz, Ufuk Turan Kürşat; Yüksel, Ahmet; Çetinkaya, Ayhan; Velioğlu, Yusuf; Uçaroğlu, Erhan Renan; Erdem, KemalettinObjective To examine dynamic thiol/disulphide homeostasis metrics as a novel risk factor of oxidative stress in patients with peripheral arterial disease. Methods One hundred patients with lower extremity peripheral arterial disease (a study group) and 100 control subjects were included in this prospective case-control study. Participants' baseline clinical characteristics and laboratory data including some oxidant/antioxidant status parameters such as albumin, ferroxidase and myeloperoxidase, and thiol/disulphide homeostasis parameters such as native thiol, total thiol and disulphide, as well as native thiol/total thiol, disulphide/native thiol and disulphide/total thiol ratios were all recorded and then compared between the groups. Results Mean albumin and ferroxidase, and median myeloperoxidase levels were found to be significantly higher in patients with the peripheral arterial disease than in control group (p = 0.045,p = 0.000 andp = 0.000, respectively). Mean native thiol and total thiol, and median disulphide levels were found to be significantly lower in the study group as compared with the control group (p = 0.000,p = 0.000 andp = 0.037, respectively). According to the results of logistic regression analysis, systolic blood pressure, ferroxidase and myeloperoxidase levels were detected to be the independent predictors of peripheral arterial disease. Conclusion Our report is the first one in the literature investigating dynamic thiol/disulphide homeostasis metrics as a novel risk factor of oxidative stress in peripheral arterial disease. Dynamic thiol/disulphide homeostasis metrics may be used as a valuable risk factor of oxidative stress in patients with the peripheral arterial disease since it is readily available, easily calculated and relatively cheap.Öğe Effect of acute and chronic ellagic acid administration on penicillin-induced epileptiform activity in rats(Wiley, 2017) Beyazçiçek, Ersin; Ankaralı, Seyit; Beyazçiçek, Özge; Ankaralı, Handan; Çetinkaya, Ayhan[No Abstract Available]Öğe The effect of Anatolian syrup on experimentally induced acetaminophen and lipopolysaccharide associated acute kidney injury(2022) Düzcü, Selma Erdoğan; Çetinkaya, Ayhan; Efe, Muhammet; Kayiş, Seyit Ali; Bekdaş, Mervan; Yoldaş, Meyri Arzu; Tirink, Omer FarukBackground: Acute kidney injury develops as a result of various etiologies and pathological mechanisms, with a high mortality rate. This study aimed to investigate the efficacy of Anatolian syrup on histopathological variables in experimentally induced acetaminophen and lipopolysaccharide associated acute kidney injury. Methods: In this study, 5 groups were formed using 40 male Wistar albino rats (200- 220g, 2-4 months old), as follows: Sham, acetaminophen (1 g/kg intraperitoneal (ip)), 3) lipopolysaccharide (5 mg/kg ip), acetaminophen (1 g/kg ip) + Anatolian syrup (15 days orally), and lipopolysaccharide (5 mg/kg ip) + Anatolian syrup (15 days orally).Tubular atrophy, tubular dilatation, cytoplasmic vacuolization in tubular epithelial cells, tubular epithelial cell necrosis, interstitial inflammation, congestion, hemorrhage, glomerular damage and loss of brushy border were evaluated histopathologically semiquantitatively using scoring from 0 to 5. Results: In histopathological variables, tubular atrophy, tubular dilatation, cytoplasmic vacuolization, necrosis, congestion, hemorrhage, glomerular damage, and loss of brushy border were significantly reduced in the acetaminophen + Anatolian syrup group compared to the acetaminophen group and in the lipopolysaccharide + Anatolian syrup group compared to the lipopolysaccharide group (p<0.001). Conclusions: The Anatolian syrup was shown to protect histopathological variables in kidney damage caused by acetaminophen and lipopolysaccharide.Öğe The effect of protocatechuic acid on ovarian histopathology and reserve in rat ovarian torsion model(AEPRESS SRO, 2023) Erimşah, Sevilay; Çetinkaya, AyhanOBJECTIVES: The aim of the study is to investigate the effects of Protocatechuic Acid (PCA), which is an antioxidant, anti-inflammatory and anti-apoptotic agent, on ovarian tissue and ovarian reserve against ischemia-reperfusion (IR) injury in a rat ovarian torsion model.BACKGROUND: Reactive oxygen radicals cause histopathological changes in the ovarian tissue during the reperfusion phase. PCA may have protective effects on ovarian tissue and reserve due to its antioxidant and antiapoptotic properties.METHODS: A total of 24 Wistar adult female rats were divided into 3 groups as the control (sham operation, n = 8), IR (Ischemia-Reperfusion, n = 8), and IR+PCA (Ischemia-Reperfusion + 80 mg/kg protocatechuic acid, n = 8). The IR and IR + PCA groups underwent 3 hours of ischemia followed by 3 hours of ovarian reperfusion. Protocatechuic acid (80 mg/kg) was administered to the IR+PCA group 30 minutes before reperfusion. After reperfusion, the ovaries were removed for histopathological and biochemical examination.RESULTS: Histopathological score and TUNEL+ cell count were significantly lower and AMH expression level was significantly higher in the IR+PCA group when compared to the IR group (p <0.05). However, in the comparison of the follicle counts, there was no statistically signifi cant difference between all groups. Due to the increase in antioxidant activity, the MDA levels were found to be significantly lower in the IR+PCA group compared to the IR group (p < 0.05).CONCLUSION: Protocatechuic acid may be an effective antioxidant in protecting ovarian tissue and follicle reserve against IR injury of the ovary (Tab. 1, Fig. 4, Ref. 36). T ext in PDF www.elis.sk
