Cytotoxic effects of calcitriol on osteosarcoma cells
Küçük Resim Yok
Tarih
2022
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: It was aimed to examine the effect of calcitriol which has known anticancer activities, on the osteosarcoma cell line SAOS-2 cells [1,25(OH)2D3]. Methods: SAOS-2 cells were grown in culture in conventional culture flasks in DMEM medium at 37°C and 5% CO2. When the cells were 70-80% confluent, morphological changes were examined under an inverted microscope. The cells were passaged into 96 microplates, and after passage, different concentrations of calcitriol was applied to the cells (0.1; 0.5; 1; 5; 10; 25 nM/ml) was done. After administration, cytotoxic effect and proliferation rates/cell proliferation were analyzed by MTT method. Results: The effect of calcitriol applied at different concentrations in cultured cells were 0.1, 1, 10, 25 nM/ml doses, in these groups proliferation was found to be statistically significantly reduced compared to the control group (p?0.05). Conclusion: Our data imply that the antiproliferative effects of vitamin D applications can be benefited from in exosomal, combined and supportive treatments in osteosarcoma. Further trials are warranted to confirm and validate our findings.
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Kaynak
Northwestern Medical Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
2
Sayı
3
