Cytotoxic effects of calcitriol on osteosarcoma cells
| dc.contributor.author | Çelik, Humeyra | |
| dc.contributor.author | Çetinkaya, Ayhan | |
| dc.contributor.author | Celik, Ilhan | |
| dc.date.accessioned | 2024-09-25T20:24:03Z | |
| dc.date.available | 2024-09-25T20:24:03Z | |
| dc.date.issued | 2022 | |
| dc.department | Abant İzzet Baysal Üniversitesi | en_US |
| dc.description.abstract | Introduction: It was aimed to examine the effect of calcitriol which has known anticancer activities, on the osteosarcoma cell line SAOS-2 cells [1,25(OH)2D3]. Methods: SAOS-2 cells were grown in culture in conventional culture flasks in DMEM medium at 37°C and 5% CO2. When the cells were 70-80% confluent, morphological changes were examined under an inverted microscope. The cells were passaged into 96 microplates, and after passage, different concentrations of calcitriol was applied to the cells (0.1; 0.5; 1; 5; 10; 25 nM/ml) was done. After administration, cytotoxic effect and proliferation rates/cell proliferation were analyzed by MTT method. Results: The effect of calcitriol applied at different concentrations in cultured cells were 0.1, 1, 10, 25 nM/ml doses, in these groups proliferation was found to be statistically significantly reduced compared to the control group (p?0.05). Conclusion: Our data imply that the antiproliferative effects of vitamin D applications can be benefited from in exosomal, combined and supportive treatments in osteosarcoma. Further trials are warranted to confirm and validate our findings. | en_US |
| dc.identifier.doi | 10.54307/NWMJ.2022.63835 | |
| dc.identifier.endpage | 167 | en_US |
| dc.identifier.issn | 2757-7724 | |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.startpage | 163 | en_US |
| dc.identifier.trdizinid | 1173306 | en_US |
| dc.identifier.uri | https://doi.org/10.54307/NWMJ.2022.63835 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/tr/yayin/detay/1173306 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12491/16288 | |
| dc.identifier.volume | 2 | en_US |
| dc.indekslendigikaynak | TR-Dizin | en_US |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Northwestern Medical Journal | en_US |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.snmz | YK_20240925 | en_US |
| dc.title | Cytotoxic effects of calcitriol on osteosarcoma cells | en_US |
| dc.type | Article | en_US |
