Morphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fields

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dc.authorid0000-0002-7217-9092
dc.contributor.authorSmaha, Djamel
dc.contributor.authorMokrini, Fouad
dc.contributor.authorİmren, Mustafa
dc.contributor.authorMokabli, Aissa
dc.contributor.authorDababat, A. Dababat
dc.date.accessioned2021-06-23T18:51:30Z
dc.date.available2021-06-23T18:51:30Z
dc.date.issued2019
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractCereal cyst nematodes (Heterodera spp.) are distributed globally and cause severe production losses of small grain cereals. To investigate the occurrence of cereal cyst nematodes in wheat-growing areas of Algeria, a survey was conducted and 27 cereal cyst nematode populations were collected. The populations were initially identified based on their morphological and morphometric characters, followed by molecular methods using species-specific primers, complemented by ITS-rDNA sequences. The morphological and morphometric features of second-stage juveniles (J2s) and cysts supported the presence of three Heterodera species: H. avenae, H. filipjevi and H. hordecalis. All morphological values of these distinct populations were very similar to those previously described for these species. Using species-specific primers for H. avenae and H. filipjevi, the specific bands of 109 bp and 646 bp confirmed the morphological identification of both species, respectively. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 27 populations. These sequences were compared with those of Heterodera species available in the GenBank database (www.ncbi.nlm.nih.gov) and re-confirmed the identity of the species. Nineteen sequences of ITS-rDNA were similar (99–100%) to the sequences of H. avenae published in the GenBank, six sequences were similar (99–100%) to H. hordecalis, and two were similar (98–99%) to H. filipjevi. The results of this study are of great value to breeding programs and extension services, where they will contribute to the design of control measures to keep damaging nematodes in check. © 2019 Polish Academy of Sciences. All rights reserved.en_US
dc.description.urihttps://www.proquest.com/publiccontent/docview/2650303245?pq-origsite=summon&parentSessionId=btzGzOyCY5OQEEjiUbCmwUKyru8aAMa6lx1JJd4h3mk%3D
dc.identifier.doi10.24425/jppr.2019.130809
dc.identifier.endpage411en_US
dc.identifier.issn1427-4345
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85074578519en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage400en_US
dc.identifier.urihttps://doi.org/10.24425/jppr.2019.130809
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3828
dc.identifier.volume59en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorİmren, Mustafa
dc.language.isoenen_US
dc.publisherPolish Academy of Sciencesen_US
dc.relation.ispartofJournal of Plant Protection Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCerealsen_US
dc.subjectCyst Nematodeen_US
dc.subjectHeterodera SPPen_US
dc.subjectITS-rDNAen_US
dc.subjectWheaten_US
dc.titleMorphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fieldsen_US
dc.typeArticleen_US

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