Enhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal plant

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dc.authorscopusid57199473515
dc.authorscopusid35271895900
dc.authorscopusid57281126300
dc.authorscopusid6603953214
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorGantait, Saikat
dc.contributor.authorMukherjee, Epsita
dc.contributor.authorGurel, Ekrem
dc.date.accessioned2024-09-25T19:45:02Z
dc.date.available2024-09-25T19:45:02Z
dc.date.issued2022
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractLycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super fruit” or “super food”. Considering the importance of the economic utility, the potential of different explants (hypocotyl, leaf and root) of L. barbarum for plant regeneration and somatic embryogenesis has been evaluated in the present study. Two sets of experiments were carried out; the first compared Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D), N6-benzylaminopurine, thidiazuron (TDZ), kinetin, and zeatin alone, while the second set tested the combinations of TDZ with 2,4-D to induce callus and subsequent shoot or embryo formation, respectively. For callus and subsequent shoot induction (for the first set of the experiment), 2,4-D was the most effective for callus induction (100%), while TDZ at 1 mg/L produced a mean of 5 shoots per callus. For the second set of experiment spontaneous induction of somatic embryos as well as subsequent maximum shoot regeneration was recorded in 0.25 mg/L 2,4-D+ 1 mg/L TDZ supplemented MS medium. Hypocotyl explant proved to be the most responsive organ that induced as many as 6 somatic embryos and subsequent 9 regenerated shoots per callus. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse. Total phenolic content was measured from shoot, brown callus and white callus of L. barbarum L. grown in MS medium supplemented with auxin alone (for callus) and auxin-cytokinin (for shoots). The highest amount of total phenolic content (640 mg GAE/g DW) was obtained in the shoot. The described protocol provides a simple way to regenerate plants through direct and indirect organogenesis as well as somatic embryogenesis, which would be useful for mass propagation, large-scale production of secondary metabolites, germplasm conservation, and genetic transformation studies in this medicinally important species. © 2022, The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS).en_US
dc.identifier.doi10.1007/s12892-022-00150-8
dc.identifier.endpage555en_US
dc.identifier.issn1975-9479
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85127360868en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage547en_US
dc.identifier.urihttps://doi.org/10.1007/s12892-022-00150-8
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12800
dc.identifier.volume25en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Crop Science and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAcclimatizationen_US
dc.subjectGoji berryen_US
dc.subjectHistologyen_US
dc.subjectHypocotylen_US
dc.subjectSecondary metabolitesen_US
dc.titleEnhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal planten_US
dc.typeArticleen_US

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