Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge

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dc.authorid0000-0002-7050-4082
dc.authorid0000-0002-2150-2726
dc.contributor.authorHarding, Stephen E
dc.contributor.authorGillis, Richard B.
dc.contributor.authorAlmutairi, Fahad
dc.contributor.authorErten, Tayyibe
dc.contributor.authorKök, Mehmet Şamil
dc.date.accessioned2021-06-23T18:55:02Z
dc.date.available2021-06-23T18:55:02Z
dc.date.issued2015
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractSedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10-12 M to 10-1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions. © 2015 by the authors; licensee MDPI, Basel, Switzerland.en_US
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council, BBSRC: BB/L025477/1en_US
dc.identifier.doi10.3390/biology4010237
dc.identifier.endpage250en_US
dc.identifier.issn2079-7737
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84961345243en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage237en_US
dc.identifier.urihttps://doi.org/10.3390/biology4010237
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4658
dc.identifier.volume4en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKök, Mehmet Şamil
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofBiologyen_US
dc.relation.publicationcategoryDiğeren_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbohydrateen_US
dc.subjectHydrodynamicsen_US
dc.subjectInteractionen_US
dc.subjectNucleic Aciden_US
dc.subjectProteinen_US
dc.titleRecent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifugeen_US
dc.typeReview Articleen_US

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