Comparison of the Effects of Digoxin and Bevacizumab on Oxygen-Induced Retinopathy Rat Model

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dc.authorscopusid15062487300
dc.authorscopusid57225780436
dc.authorscopusid6506789098
dc.authorscopusid57381259600
dc.authorscopusid35767198900
dc.authorscopusid57193008084
dc.contributor.authorBalbaba, Mehmet
dc.contributor.authorGuler, Seda Duran
dc.contributor.authorUlas, Fatih
dc.contributor.authorCanleblebici, Mehmet
dc.contributor.authorYıldırım, Hakan
dc.contributor.authorİncili, Canan Akdeniz
dc.date.accessioned2024-09-25T19:43:17Z
dc.date.available2024-09-25T19:43:17Z
dc.date.issued2023
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractPurpose: To evaluate the effect of digoxin on oxygen-induced retinopathy (OIR) model and compare the results with bevacizumab treatment. Methods: Twenty-eight newborn Sprague-Dawley rats were randomly divided into four groups:control group, normoxia+intraperitoneal (ip) normal saline (NS); OIR group, OIR+ip NS; OIR+digoxin group, OIR+ip 0.1 mg/kg of digoxin; and OIR+bevacizumab group, OIR+ip 2.5 mg/kg of bevacizumab. The rats were exposed to 50% oxygen for 24 h, followed by 10% oxygen for 24 h, to induce OIR. This cycle was repeated seven times until 14 days postnatal (P). In all groups, single-dose injections were administered on P15th day. Histopathological and immunohistochemical examinations were performed at the end of the study. Results: The mean neovascular cell nuclei counts (NVCN) of the four groups were 9.00±3.16, 41.80±11.44, 19.38±2.20, and 16.00±2.62. The mean NVCN count was significantly reduced in the treatment groups compared to the OIR group (p<0.001). The mean NVCN count was similar between the treatment groups (p=0.078). In immunohistochemical staining, the immunoreactivity values of the vascular endothelial growth factor (VEGF) were 0.01±0.00, 1.65±0.30, 0.09±0.08, and 0.04±0.02, and the tumor necrosis factor alpha (TNF-?) values were 0.10±0.00, 1.12±0.18, 0.18±0.13, and 0.14±0.05. In the OIR group, VEGF and TNF-? immunoreactivity increased markedly compared to the control group (p<0.001). VEGF and TNF-? immunoreactivity of the treatment groups decreased significantly compared to the OIR group (p<0.001). VEGF and TNF-? immunoreactivity were similar between the treatment groups (VEGF:p=0.752; TNF-?:p=0.099). Conclusions: Retinal neovascularization was significantly suppressed by digoxin treatment, and this effect was comparable to bevacizumab in the OIR model. © 2023 Gazi Eye Foundation. All rights reserved.en_US
dc.identifier.doi10.37845/ret.vit.2023.32.18
dc.identifier.endpage109en_US
dc.identifier.issn1300-1256
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85166941089en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage103en_US
dc.identifier.trdizinid1185295en_US
dc.identifier.urihttps://doi.org/10.37845/ret.vit.2023.32.18
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1185295
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12523
dc.identifier.volume32en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGazi Eye Foundationen_US
dc.relation.ispartofRetina-Vitreusen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectbevacizumaben_US
dc.subjectdigoxinen_US
dc.subjectoxygen-induced retinopathyen_US
dc.subjecttumor necrosis factor-?en_US
dc.subjectvascular endothelial growth factoren_US
dc.titleComparison of the Effects of Digoxin and Bevacizumab on Oxygen-Induced Retinopathy Rat Modelen_US
dc.typeArticleen_US

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