Xylitol production from chestnut shell hydrolysate by Candida tropicalis: modelling the effects of fermentation parameters on maximum xylitol concentration
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Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Xylitol is a sugar alcohol having many commercial applica-tions because of its sweetness, low calorie, low glycemic index and anticariogenic properties. It is widely used in the food and pharma- ceutical industries as an alternative sweetener. Xylitol production is expanding worldwide and currently a strong demand is esti-
mated for this product in the global market. On the other hand, its relatively high prices are promoting the search for production processes with lower costs. It can be produced by biotechnologic processes with the use of hemicellulosic hydrolysates. In this study, hemicellulosic hydrolysate produced from chestnut shell was used for xylitol production by the strain Candida tropicalis M2. The hydrolysate was prepared by acid hydrolysis followed by several detoxification steps (overliming and active charcoal treatment).The prepared hydrolysate was fortified with ammonium chloride as nitrogen source. A central composite design was conducted for determining the effects of initial xylose concentration, added ammonium chloride concentration and shaking rate on maximum
xylitol concentration. Experiments were carried out in water bath shakers using 300 mL cotton-plugged flasks containing 80 mL of fermentation medium. The effects of independent variables were defined by a quadratic model. According to the ANOVA results, linear effects of each variables were found to be significant for max-
imum xylitol concentration. In addition, interaction effects of all parameters, as well as quadratic effects of added ammonium chlo- ride concentration and shaking rate were determined as significant. It was also demonstrated that fortified chestnut shell hydrolysate could be used for xylitol production by C. tropicalis.
Açıklama
Anahtar Kelimeler
Candida Tropicalis, Xylitol, Chestnut Shell, Xylitol Concentration
Kaynak
New Biotechnology
WoS Q Değeri
Q1
Scopus Q Değeri
Cilt
33