Inhibition mechanisms of lead, cadmium and nickel for the purified bovine liver NADPH-cytochrome P450 reductase

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dc.authorid0000-0003-4314-8438en_US
dc.authorid0000-0002-6949-9191
dc.contributor.authorBozcaarmutlu, Azra
dc.contributor.authorÇelik, H.
dc.contributor.authorArınç, E.
dc.date.accessioned2021-06-23T19:27:47Z
dc.date.available2021-06-23T19:27:47Z
dc.date.issued2011
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description47th Congress of the European-Societies-of-Toxicology -- AUG 28-31, 2011 -- Paris, FRANCEen_US
dc.description.abstractVery little information is available on the effects of metals in veterinary animals, despite the fact that they are exposed to these chemicals via contaminated plants and water sources. NADPH-Cytochrome P450 reductase is an essential component of the microsomal cytochrome P450 monooxygenase system. In this study, our aim was to elucidate in vitro effect of Pb2+, Cd2+ and Ni2+ on the purified bovine NADPH-cytochrome P450 reductase. NADPH-cytochrome P450 reductase was purified from bovine liver microsomes. The effects of metals were studied by adding various concentrations of them on the reaction mixture. All of the metal ions caused inhibition of the enzyme activity. The IC50 values of Pb2+, Cd2+ and Ni2+ were estimated to be 0.027 mM, 0.059 mM and 2.9 mM, respectively. Of the metal ions tested, Pb2+ exhibited much higher inhibitory effect at lower concentrations. Kinetic analysis was done to determine type of inhibition of purified bovine cytochrome P450 reductase. All of the metal ions displayed noncompetitive type of inhibition mechanism for the purified NADPH-cytochrome P450 reductase as analyzed by Lineweaver-Burk Plot and Dixon plot. Ki values of Pb2+, Cd2+ and Ni2+ were calculated from Dixon plots as 0.03 mM, 0.04 mM and 2.0 mM, respectively. The results of this study clearly indicate that mechanism of inhibition of lead, cadmium and nickel for cytochrome P450 reductase in bovine is noncompetitive type.en_US
dc.description.sponsorshipEuropean Soc Toxicolen_US
dc.identifier.doi10.1016/j.toxlet.2011.05.664
dc.identifier.endpageS192en_US
dc.identifier.issn0378-4274
dc.identifier.startpageS192en_US
dc.identifier.urihttps://doi.org/10.1016/j.toxlet.2011.05.664
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6892
dc.identifier.volume205en_US
dc.identifier.wosWOS:000293814500612en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorBozcaarmutlu, Azra
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofToxicology Lettersen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNADPH-cytochromeen_US
dc.titleInhibition mechanisms of lead, cadmium and nickel for the purified bovine liver NADPH-cytochrome P450 reductaseen_US
dc.typeConference Objecten_US

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