Production of high purity beta amanitin
| dc.authorscopusid | 24724487100 | |
| dc.authorscopusid | 6505553942 | |
| dc.authorscopusid | 55487000800 | |
| dc.authorscopusid | 37002432900 | |
| dc.authorscopusid | 34771856000 | |
| dc.authorscopusid | 25825771800 | |
| dc.contributor.author | Kaya, Ertu?rul | |
| dc.contributor.author | Yaykaşli, Kürşat O?uz | |
| dc.contributor.author | Karahan, Selim | |
| dc.contributor.author | Bayram, Recep | |
| dc.contributor.author | Saritaş, Ayhan | |
| dc.contributor.author | Yaykaşli, Emine | |
| dc.date.accessioned | 2024-09-25T19:45:19Z | |
| dc.date.available | 2024-09-25T19:45:19Z | |
| dc.date.issued | 2012 | |
| dc.department | Abant İzzet Baysal Üniversitesi | en_US |
| dc.description.abstract | Objective: Beta amanitin has been used in experiment and has been purified only around 90% purity using existing methods. In this study, it has been aimed to describe the method in order to produce high-purity beta amanitin using preparative HPLC. Methods: Amanita phalloides mushrooms hve been collected, extracted and purified 2 times using preparative HPLC. Validation of the toxin has been performed by comparison of retention time at HPLC and ultraviolet spectrum. Results: Beta amanitin was obtained with 91% (±2.36) purity after first purification process. Beta amanitin was obtained with 99,2% (±0.38) purity after second purification process. It seemed that purified toxin and standard were given maximum absorbance at 303 nm and minumum absorbans at 263 nm, and the structure of the spectrums for both was similar. Conclusion: Beta amanitin with >%99 purity can be produced by this method. © 2012 Düzce Medical Journal. | en_US |
| dc.identifier.endpage | 53 | en_US |
| dc.identifier.issn | 1307-671X | |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.scopus | 2-s2.0-84876928397 | en_US |
| dc.identifier.scopusquality | Q4 | en_US |
| dc.identifier.startpage | 48 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.12491/12967 | |
| dc.identifier.volume | 14 | en_US |
| dc.indekslendigikaynak | Scopus | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Duzce University Medical School | en_US |
| dc.relation.ispartof | Duzce Medical Journal | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.snmz | YK_20240925 | en_US |
| dc.subject | Beta amanitin | en_US |
| dc.subject | Preparative HPLC | en_US |
| dc.subject | Purification | en_US |
| dc.title | Production of high purity beta amanitin | en_US |
| dc.title.alternative | Yüksek saflıkta beta amanitin üretimi] | en_US |
| dc.type | Article | en_US |
