A crystal quartz microbalance biosensor system to detect triple negative human breast cancer cells
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Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mary Ann Liebert, Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Breast cancer is the most common cancer in women. The highly
metastatic breast cancer cells are usually difficult to combat with and
they usually lead to casualties. Therefore, it may be of value to have a
system for early detection of this type of cells. It was aimed to prepare
a quartz microbalance biosensor to detect highly metastatic cells,
namely MDA-MB 231 cells by targeting their transferrin receptors.
The gold surface of the QCM biosensor chip was functionalized by
the attachment of transferrin coated Poly(2-hydroxyethyl methacrylate) (pHEMA) nanoparticles. Nanoparticles were characterized by
zeta sizer analysis. Nanoparticles without transferrin on their surface
were used as control. The QCM sensor systems were analysed by
using atomic force microscope (AFM), elypsometer and FTIR-ATR.
It was seen that the nanoparticles formed a monolayer over the surface. Following characterization studies the sensors prepared with or
without transferrin were tested for their ability to detect invasive
breast cancer cells. The binding affinity and kinetics were determined. Adsorbtion kinetics were found to obey Langmuir- Freundlich model. The biosensor was tested with MCF 7 non invasive
breast cancer cells and MDA MB 231 cells at prolonged serum
deprivation in order to test selectivity. It is concluded that, this biosensor is highly sensitive and selective for vital MDA-MB 231
metastatic human breast cancer cells. Thus, this system may be used
to test blood samples for early detection of circulating metastatic
breast cancer cells.
Açıklama
TERMIS - Americas Conference and Exhibition -- DEC 11-14, 2016 -- San Diego, CA
Anahtar Kelimeler
Kaynak
Tissue Engineering Part A
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
22
