A novel capillary gel electrophoresis based fragment analysis method for the rapid detection of important thrips species on alfalfa in Turkey

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dc.authorscopusid57225990687
dc.authorscopusid57207861800
dc.authorscopusid36440878700
dc.authorscopusid14013076000
dc.authorscopusid57208189821
dc.contributor.authorMorca, Ali Ferhan
dc.contributor.authorYücel, Cenk
dc.contributor.authorBariş, Aydemir
dc.contributor.authorAtakan, Ekrem
dc.contributor.authorÇelik, Ali
dc.date.accessioned2024-09-25T19:43:04Z
dc.date.available2024-09-25T19:43:04Z
dc.date.issued2022
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractThrips cause significant yield reduction in several industrial crops. Since these pests are also included in the quarantine organisms of Turkey, the rapid detection of agents is important to prevent their spread to new areas. Mitochondrial cytochrome oxidase I (COI) barcoding gene assay; one of the molecular methods is widely used in thrips identification. However, as the COI gene has a very short fragment length, it is very difficult to distinguish fragment sizes on agarose gel after PCR. In this study, a new identification method was developed by integrating the Capillary Gel Electrophoresis (CGE) system for Thrips tabaci Lideman, Frankliniella occidentalis (Pergande) and Frankliniella intonsa (Trybom) species, using primer pairs previously used by different researchers. The assay produces strong signals obtained by minimizing the margin of error in the separation of fragment lengths close to each other, especially in the short fragment length COI gene. Therefore, by eliminating the gel electrophoresis step, reliable detections could be obtained without exposure to hazardous chemicals. The novel method shortened the detection time and minimized the process mistakes on the detection of a single thrips with a low DNA concentration. Total 83 thrips individual (52 F. intonsa, 31 F. occidentalis) were able to be detected with this capillary gel electrophoresis based fragment analysis. The novel method is evaluated as unique, specific and quick for the detection of three different thrips species. It is also thought to be able to utilize for identification of different thrips species with short fragment sizes in the foreseeable future. © 2022, Plant Protection Central Research Institute. All rights reserved.en_US
dc.description.sponsorshipDirectorate of Central Plant Protection Research Institute, (TAGEM-BS-14/09-02/02-08); General Directorate of Agricultural Research and Policiesen_US
dc.identifier.doi10.16955/bitkorb.1078737
dc.identifier.endpage11en_US
dc.identifier.issn0406-3597
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85163022042en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage5en_US
dc.identifier.urihttps://doi.org/10.16955/bitkorb.1078737
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12412
dc.identifier.volume62en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPlant Protection Central Research Instituteen_US
dc.relation.ispartofPlant Protection Bulletin (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectFrankliniella intonsaen_US
dc.subjectFrankliniella occidentalisen_US
dc.subjectmitochondrial cytochrome oxidase Ien_US
dc.subjectmultiplex polymerase chain reactionen_US
dc.subjectThysanopteraen_US
dc.titleA novel capillary gel electrophoresis based fragment analysis method for the rapid detection of important thrips species on alfalfa in Turkeyen_US
dc.title.alternativeTürkiye'de yoncada önemli thrips türlerinin hızlı tespiti için yeni bir kapiler jel elektroforez tabanlı fragment analiz yöntemi]en_US
dc.typeArticleen_US

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