Purification of High Purity Alpha Amanitin Using Preparative HPLC Method

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dc.authoridKarahan, Selim/0000-0001-5784-7091
dc.contributor.authorKaya, Ertugrul
dc.contributor.authorKarahan, Selim
dc.contributor.authorYaykasli, Kursat Oguz
dc.contributor.authorBayram, Recep
dc.contributor.authorSaritas, Ayhan
dc.date.accessioned2024-09-25T19:56:14Z
dc.date.available2024-09-25T19:56:14Z
dc.date.issued2012
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractObjective: Alpha-amanitin is purified only around 90% purity using existing methods. In this study, it was aimed to describe the method in order to obtain high-purity alpha-amanitin using preparative HPLC. Methods: Amanita phalloides mushroom was collected, extracted and purified 2 times using preparative HPLC. Validation of the toxin was performed by comparison of retention time and ultraviolet spectrum at HPLC. Results: Alpha-amanitin was obtained with 93% (+/- 1.24) purity after first purification process. Alpha-amanitin was obtained with 99,8% (+/- 0.26) purity after second purification process. It was seemed that purified toxin and standard were given maximum absorbance at 303 nm and minimum absorbance at 263 nm, and the structure of the spectrums for both was similar. Conclusion: Alpha-amanitin with >99% purity can be obtained by this method at low cost.en_US
dc.identifier.endpage41en_US
dc.identifier.issn1309-3878
dc.identifier.issue3en_US
dc.identifier.startpage35en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13199
dc.identifier.volume4en_US
dc.identifier.wosWOS:000420942500006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherDuzce Univen_US
dc.relation.ispartofKonuralp Tip Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAlpha amanitinen_US
dc.subjectPreparative HPLCen_US
dc.subjectPurificationen_US
dc.titlePurification of High Purity Alpha Amanitin Using Preparative HPLC Methoden_US
dc.typeArticleen_US

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