Einkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocol

Basit Öğe Kaydı
dc.authorid0000-0002-9446-7538
dc.authorid0000-0001-5245-6294
dc.authorid0000-0003-0060-259X
dc.authorid0000-0003-1685-6711
dc.authorid0000-0003-3460-7575
dc.contributor.authorÖrgeç, Mehmet
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorŞahin, Günce
dc.contributor.authorAğıl, Ferdi
dc.contributor.authorZencirci, Nusret
dc.date.accessioned2021-06-23T18:32:35Z
dc.date.available2021-06-23T18:32:35Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractEinkorn is one of the oldest and important wheat species because of its increasing economic value, agronomical technical properties, and health and sanitary effects. Therefore, a faster tissue culture production protocol which may ensure a fast einkorn production is desired. The tissue culture process first requires an efficient sterilization technique of the explant to eliminate contamination factors in this hulled species. In this study, we, thus, aimed to determine the effective einkorn sterilization process for the removal of contaminating microorganisms without the loss of germination capacity and viability. We tested 22 following sterilant combinations with different concentration and exposure time: Ethanol, Commercial Bleach, Tween 20, Mercury (II) Chloride, and Sterile Distilled Water. On the other hand, obtained the data were analyzed by one-way ANOVA and Duncan test, where p?0.05 was accepted significant. Forty percent commercial bleach which were applied fifteen minutes eliminated all microorganisms with a 92.6% seed germination. The second-best technique was one minute exposure of 70% ethanol which were followed by 10 minutes exposure of 20% commercial bleach. This assured up to 89% germination and no contamination. The worst results came from the mercury (II) chloride with 0% contamination and ? 25% germination rate. Mercury (II) Chloride was both highly toxic on the seeds and reduced the contamination. Commercial Bleach and Ethanol Chemicals were less toxic while providing an acceptable sterilizationen_US
dc.identifier.endpage74en_US
dc.identifier.issn2148-6905
dc.identifier.issn2148-6905
dc.identifier.issue2en_US
dc.identifier.startpage67en_US
dc.identifier.trdizinid275317en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpjMU16RTNOdz09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/1939
dc.identifier.volume5en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorÖrgeç, Mehmet
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorŞahin, Günce
dc.institutionauthorAğıl, Ferdi
dc.institutionauthorZencirci, Nusret
dc.language.isoenen_US
dc.relation.ispartofInternational Journal of Secondary Metaboliteen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiyolojien_US
dc.subjectContamination
dc.subjectEinkorn Wheat
dc.subjectGermination
dc.subjectSeed Sterilization
dc.subjectTissue Culture
dc.titleEinkorn (Triticum monococcum ssp. monococcum) in vitro propagation sterilization protocolen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
mehmet-orgec.pdf
Boyut:
1.08 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/ Full text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Biyoloji Bölümü Koleksiyonu
Tarla Bitkileri Bölümü Koleksiyonu