Insight into Population Structure and Evolutionary Analysis of the Emerging Tomato Brown Rugose Fruit Virus

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dc.authorscopusid57208189821
dc.authorscopusid57193531110
dc.authorscopusid57225990687
dc.authorscopusid57217302011
dc.authorscopusid55863373100
dc.contributor.authorÇelik, Ali
dc.contributor.authorCoşkan, Sevgi
dc.contributor.authorMorca, Ali Ferhan
dc.contributor.authorSantosa, Adyatma Irawan
dc.contributor.authorKoolivand, Davoud
dc.date.accessioned2024-09-25T19:45:06Z
dc.date.available2024-09-25T19:45:06Z
dc.date.issued2022
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractA total of 112 symptomatic tomatoes (Solanum lycopersicum L.) and 83 symptomatic pepper (Capsicum spp.) samples were collected in Ankara, Eskişehir, Bartın, and Zonguldak provinces of Turkey during 2020–2021. Six tomatoes and one pepper sample (3.6%) tested positive for tomato brown rugose fruit virus (ToBRFV, genus Tobamovirus) infection by DAS-ELISA and RT-PCR. ToBRFV-positive tomato and pepper plants were removed from greenhouses as soon as possible, and the greenhouses and tools were disinfected completely. Phylogenetic analysis on the complete CP sequences suggested the clustering of 178 GenBank isolates and 7 novel isolates into three groups. A study using DnaSP software showed very low genetic variation among current global ToBRFV isolates. All four ORFs of the virus genome were under strong negative evolutionary constraints, with a ? value range of 0.0869–0.2066. However, three neutrality tests indicated that most populations of the newly identified ToBRFV are currently expanding by assigning statistically significant negative values to them. The very low FST values (0.25 or less) obtained by all comparisons of the isolates from Europe, the Middle East, China, and America concluded that there is no clear genetic separation among currently known isolates from different geographic origins. The divergence time of ToBRFV was estimated to be in the middle of the course of the evolution of 11 tested tobamoviruses. The time to the most recent common ancestors (TMRCAs) of ToBRFV were calculated to be 0.8 and 1.87 with the genetically closest members of Tobamovirus. The results of this study could improve our understanding on the population structure of the emerging ToBRFV. © 2022 by the authors.en_US
dc.description.sponsorshipFaculty of Agriculture, Universitas Gadjah Mada; General Directorate of Agricultural Research and Policies, (TAGEM/BSAD/Ü/21/A2/P1/5064); Ministry of Agriculture and Forestry of Republic of Turkey, (3612/UN1/PN/PN/PT.01.03/2022, E-36871779-320.03.05-4502631)en_US
dc.identifier.doi10.3390/plants11233279
dc.identifier.issn2223-7747
dc.identifier.issue23en_US
dc.identifier.scopus2-s2.0-85143640042en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/plants11233279
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12861
dc.identifier.volume11en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofPlantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectdivergence timeen_US
dc.subjectevolutionary constraintsen_US
dc.subjectgenetic variationen_US
dc.subjectphylogenetic analysisen_US
dc.titleInsight into Population Structure and Evolutionary Analysis of the Emerging Tomato Brown Rugose Fruit Virusen_US
dc.typeArticleen_US

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