Bovine Serum Albumin Adsorption by Dye Derived Poly(hydroxyethyl methacrylate) [PHEMA] Membranes

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dc.contributor.authorÇimen, Duygu
dc.contributor.authorYılmaz, Fatma
dc.date.accessioned2024-09-25T20:19:54Z
dc.date.available2024-09-25T20:19:54Z
dc.date.issued2015
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractProcion Brown MX 5BR (PBR) attached poly(hydroxyethyl methacrylate) [PHEMA] membranes were used for adsorption of albumin. The poly(hydroxyethyl methacrylate) was selected as the basic component due to it’s inertness, mechanical strength, chemical and biological stability, and biocompatibility. Different amounts of PBR was covalently attached to the poly(hydroxyethyl methacrylate) membrane to prepare poly(hydroxyethyl methacrylate)-Procion Brown [PHEMA-PBR] membrane. PHEMA-PBR membrane was characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Dye attached PHEMA membranes were used in bovine serum albumin (BSA) adsorption studies to assess the effects of pH, protein concentration, flow rate, temperature and ionic strength. The maximum albumin adsorption of PBR attached PHEMA membrane was 14.79 mg/g at pH 7.0 from aqueous solutions. Desorption of albumin was studied with 1.0 M NaCI solution in a continuous system. The reusability of the adsorption medium was tested for 4 adsorption-desorption cycles. Adsorption isotherms fitted Freundlich adsorption model and adsorption kinetics followed pseudo-second order model.en_US
dc.identifier.doi10.15671/HJBC.20154314244
dc.identifier.endpage223en_US
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue3en_US
dc.identifier.startpage213en_US
dc.identifier.trdizinid488166en_US
dc.identifier.urihttps://doi.org/10.15671/HJBC.20154314244
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/488166
dc.identifier.urihttps://hdl.handle.net/20.500.12491/15824
dc.identifier.volume43en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHacettepe Journal of Biology and Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.titleBovine Serum Albumin Adsorption by Dye Derived Poly(hydroxyethyl methacrylate) [PHEMA] Membranesen_US
dc.typeArticleen_US

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