Bovine Serum Albumin Adsorption by Dye Derived Poly(hydroxyethyl methacrylate) [PHEMA] Membranes
Küçük Resim Yok
Tarih
2015
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Procion Brown MX 5BR (PBR) attached poly(hydroxyethyl methacrylate) [PHEMA] membranes were used for adsorption of albumin. The poly(hydroxyethyl methacrylate) was selected as the basic component due to it’s inertness, mechanical strength, chemical and biological stability, and biocompatibility. Different amounts of PBR was covalently attached to the poly(hydroxyethyl methacrylate) membrane to prepare poly(hydroxyethyl methacrylate)-Procion Brown [PHEMA-PBR] membrane. PHEMA-PBR membrane was characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Dye attached PHEMA membranes were used in bovine serum albumin (BSA) adsorption studies to assess the effects of pH, protein concentration, flow rate, temperature and ionic strength. The maximum albumin adsorption of PBR attached PHEMA membrane was 14.79 mg/g at pH 7.0 from aqueous solutions. Desorption of albumin was studied with 1.0 M NaCI solution in a continuous system. The reusability of the adsorption medium was tested for 4 adsorption-desorption cycles. Adsorption isotherms fitted Freundlich adsorption model and adsorption kinetics followed pseudo-second order model.
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Kaynak
Hacettepe Journal of Biology and Chemistry
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Scopus Q Değeri
Cilt
43
Sayı
3
