Insertion of an antimicrobial gene into Agrobacterium and its further use in transforming tobacco

dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T18:40:12Z
dc.date.available2021-06-23T18:40:12Z
dc.date.issued2001
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractIn the present work, we first describe the transfer of a novel gene, designated 5W, into the LBA4404 Agrobacterium tumefaciens strain using pPCV91 plasmid through a triparental mating system. The 5W gene was recently isolated from macadamia, a native nut tree species of Australia, and has been shown to have an antimicrobial effect against certain fungi in vitro. Secondly, this gene was tranferred into tobacco (Nicotiana tabacum cv. Xhantii) using leaf explants to develop a system which could then be used for transferring this antimicrobial peptide into sunflower cultivars.en_US
dc.identifier.endpage175en_US
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue4en_US
dc.identifier.startpage169en_US
dc.identifier.trdizinid27838en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpjNE16ZzQ
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3006
dc.identifier.volume25en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMacademiaen_US
dc.subjectTobacco
dc.subjectTriparental Mating
dc.subjectGene Transfer
dc.subjectLeaf Explants
dc.titleInsertion of an antimicrobial gene into Agrobacterium and its further use in transforming tobaccoen_US
dc.title.alternativeAntimikrobiyal bir genin Agrobacterium’a yerleştirilmesi ve tütünün transformasyonunda kullanılmasıen_US
dc.typeArticleen_US

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