Ossification and growth of the ribs and sternum in prehatching period of the hubbert strain broiler
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Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Son yıllarda kanatlılar üzerinde yapılan deneysel embriyolojik çalışmalar özellikle iskelet mutasyonları, yapay ortamlarda embriyo kültürlerinin gelişimi ve yeni ilaçların teratolojik etkilerini içermektedir. Normal iskelet gelişimi verileri, bu çalışmaların yorumlanması ve doğrulanması için vazgeçilmez parametrelerdir. Araştırmada, inkubasyonun 10. gününden 20. güne kadar hergün 10 adet Hubbert (JV) ırkı embriyosu kuluçka makinesinden çıkartılarak sternum ve kostaların kemikleşmesi ve gelişimi gözlemlendi. %10’luk formaldehit solusyonunda ve % 95’lik ethanolde tespit edilen embriyolar temizlenerek, kıkırdak ve kemik dokuların ayrı renklerde tespiti için alcian blue ve alizarin red ile boyandı. İskelet sisteminin gözlenmesi ve fotoğraf çekimleri, özellikle kıkırdaklaşma ve kemikleşme zamanları dikkate alınarak, stereoskopik mikroskopla yapıldı. Kostalarda ilk kemikleşme merkezi inkubasyonun 11. gününde saptandı. Sternumun kemikleşmesinin ise 15. günde başladığı gözlendi. Çalışmada, kostalarda bulunan Proccessus uncinatus’ların çok erken kıkırdaklaşma ve kemikleşme sürecine başladığı tespit edildi. Kemikleşmiş kısımların gelişiminin inkubasyonun 20. gününe kadar devam ettiği saptandı.
Recent researches in experimental embryology of avian species have particularly investigated skeletal mutations, development of cultured embryos under artificial conditions, and teratogenic consequences of new drugs. Data on the normal skeletogenous stages are also essential parameters to interpret and elucidate the data acquired on these studies. Accordingly, a number of 10 embryos were prepared every 24 h from 10 to 20 days of incubation, to report the ossification and growth of the ribs and sternum of the Hubbert strain broiler (JV). The embryos fixed in 10% formaldehyde then 95% ethanol solutions were cleared and the skeletons of the embryos were stained in toto with Alcian blue&alizarin red for cartilage and ossified components, respectively. Observation of the skeletons photography were performed under a stereoscopic microscopy, paying particular attention to the timing of chondrification and ossification of the bones. The first occurrence of the ossification was observed in the ribs on the 11th day of incubation. Those in the sternum were seen on the 15th day. We observed in this study that the uncinate processes of the ribs showed very early chondrification and ossification. The ossified components continued to develop until the 20th day of incubation when the hatching took place.
Recent researches in experimental embryology of avian species have particularly investigated skeletal mutations, development of cultured embryos under artificial conditions, and teratogenic consequences of new drugs. Data on the normal skeletogenous stages are also essential parameters to interpret and elucidate the data acquired on these studies. Accordingly, a number of 10 embryos were prepared every 24 h from 10 to 20 days of incubation, to report the ossification and growth of the ribs and sternum of the Hubbert strain broiler (JV). The embryos fixed in 10% formaldehyde then 95% ethanol solutions were cleared and the skeletons of the embryos were stained in toto with Alcian blue&alizarin red for cartilage and ossified components, respectively. Observation of the skeletons photography were performed under a stereoscopic microscopy, paying particular attention to the timing of chondrification and ossification of the bones. The first occurrence of the ossification was observed in the ribs on the 11th day of incubation. Those in the sternum were seen on the 15th day. We observed in this study that the uncinate processes of the ribs showed very early chondrification and ossification. The ossified components continued to develop until the 20th day of incubation when the hatching took place.
Açıklama
Anahtar Kelimeler
Broyler, Kosta, Kemikleşme, Sternum, Broiler, Ossification, Rib
Kaynak
İstanbul Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
1
