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Öğe Comparison of the effects of topical corneal inhibitory agents on TTL and PON1 in rats(Rjpbcs Research Journal Pharmaceutical, Biological & Chemical Sciences, 2017) Soydan, Adem; Yazar, Hayrullah; Çetinkaya, Ayhan; Terzi, Elçin Hakan; Ulaş, Fatih; Doğan, ÜmitThe aim of this study was to compare the effects of topical corneal inhibitory agents on total thiol (TTL) and paraoxonase 1 (PON1) levels in rats with experimentally acquired keratoconjunctivitis. Thirty-five rats were divided into five groups. Twenty-four hours prior to the experiment, keratoconjunctivitis was established in the right eye of the rats using sodium hydroxide. The treatments of the five groups were as follows: group I: (control) isotonic saline (0.9%), group II: topical 0.05% cyclosporine A, group III: topical 1% diluted propolis, group IV: topical 3% diluted propolis, and group V: 0.1% dexamethasone. At the end of the 10th day, one rat in each group, except the cyclosporine group (group II), had died. The treatment was applied to all groups three times a day for 10 days. Subsequently, blood samples were obtained and used for determining the levels of TTL and PON1 (Architect C16000). All statistical analyses were performed using IBM SPSS for Windows Version 20.0 software. Descriptive statistics were calculated from the values obtained from this study and shown as arithmetic mean and standard deviation. Kruskal Wallis variance analysis was conducted. p values found to be under 0.05 were accepted as statistically significant. The study was performed after the approval [By The Animal Research Ethics Committee, Bolu Abant Izzet Baysal University, Number: 13.30.2.ABU.0.05.05-050.01.04-1, January.8.2016]. The TTL results were as follows (mu mol/L): group I: 253.24, group II: 238.70, group III: 281.39, group IV: 284.80 and group V: 260.65. No marked differences were observed between the control group and the other groups (P>0.05). The PON1 results were as follows (U/L): group I: 521.49, group II: 472.30, group III: 362.37, group IV: 327.48 and group V: 440.31. No marked difference was observed between the control group and the other groups (P > 0.05). However, there was a marked difference in the PON1 results between the 1% and 3% propolis groups. CNV inhibitor agents' effects on TTL and PON1 values were similar.Öğe Different exercise intensity and oxidative stress responses: thiol/disulfide homeostasis(Wiley, 2018) Kayacan, Yıldırım; Çetinkaya, Ayhan; Yazar, Hayrullah; Makaracı, Yücel[No Abstract Available]Öğe Evaluation of silibinin effect on methotrexate induced hepatotoxicity in rats(Wiley, 2018) Kurt, Emine; Çetinkaya, Ayhan; Turan, Gupse; Köroğlu, Mehmet; Yazar, Hayrullah[No Abstract Available]Öğe Oxidative stress response to different exercise intensity with an automated assay: thiol/disulphide homeostasis(Taylor & Francis Ltd, 2019) Kayacan, Yıldırım; Çetinkaya, Ayhan; Yazar, Hayrullah; Makaracı, YücelThe aim of this study was to investigate the effect of different intensity treadmill exercises on the thiol disulphide homeostasis which is a new marker of oxidative stress in rats. Male albino Wistar rats were randomly divided into four groups as follows: control (CNT), low (LEx), moderate (MEx) and high-intensity exercise (HEx) group. Exercise was performed for 4 weeks. Following completion of the experimental protocol, serum total thiol, native thiol and disulphide concentrations were determined using a novel automated measurement method. Additionally, dynamic disulphide status, reduced thiol, oxidised thiol and thiol oxidation reduction percentage ratios were compared among the groups. Disulphide levels were significantly lower in MEx group and highest in CNT group (p = .047). The lowest oxidised thiol and the highest reduced thiol were determined in CNT group (p = .086; p = .083). These findings indicate that moderate-intensity exercise is more effective in reducing oxidative stress than low and high-intensity exercise.Öğe Silibinin effect on methotrexate-induced hepatotoxicity in rats(Aves, 2022) Yanaşoğlu, Emine; Büyükavcı, Mustafa; Çetinkaya, Ayhan; Turan, Gupse; Köroğlu, Mehmet; Yazar, Hayrullah; Büyükokuroğlu, Mehmet EminObjective: Hepatotoxicity is one of the major side effects of methotrexate and limits its use. In this study, we investigated the hepatoprotective effect of silibinin and the role of oxidative stress markers and cytokines on high-dose methotrexate-induced hepatotoxicity in rats. Materials and Methods: In this study, rats were randomly divided into 5 groups (n = 7). Methotrexate (20 mg/kg, intraperitoneally) was administered on the first day in all groups except control. Silibinin was injected for 5 days to methotrexate-silibinin25, methotrexate-silibinin50, and methotrexate-silibinin 100 groups at a dose of 25, 50, arid 100 mg/kg/day, respectively. On the sixth day. blood and liver samples were obtained and rats were sacrificed. Serum total antioxidant capacity, total oxidant status, total thiol, native thiol, alanine aminotransferase, aspartate transaminase, bilirubin, albumin, tumor necrosis factor-alpha, and interleukin-10 levels were measured. In addition, a histopathological evaluation of liver tissues was performed. Results: Methotrexate reduced total antioxidant capacity and increased disulfide/total thiol ratio. Histopathologic examination revealed that methotrexate increased hepatic damage and 50 mg/kg/dose of silibinin prevented inflammatory cell infiltration in particular. Conclusion: Our results suggest that silibinin (50 mg/kg/day) may reduce the hepatic damage in rnethotrexate-induced hepatotoxicity in rats by increasing antioxidant capacity.
