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Öğe Biological evaluation and phytochemical profiling of some lichen species(Akademiai Kiado Zrt, 2019) Taş, İsa; Yıldırım, Arzu Birinci; Özkan, Erva; Özyiğitoğlu, Gülşah Çobanoğlu; Yavuz, Muhsine Zeynep; Türker, Arzu UçarLichens are a symbiotic relationship between a fungus and a photosynthetic partner. Chemical characterization and bioactive potentials (antiproliferative, antioxidant, and antibacterial) of five lichen species (Evernia prunastri, Platismatia glauca, Pseudevernia furfuracea, Ramalina fastigiata, and Ramalina farinacea) were assessed. Five lichen metabolites (usnic acid, atranorin, stictic acid, evernic acid, and fumarprotocetraric acid) were analyzed by HPLC-DAD. E. prunastri was noteworthy evernic acid source. Antiproliferative activity was evaluated using human breast adenocarcinoma (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines. The strongest activity was observed for P. glauca against HepG2/C3A, while the only lichen species that induced cytotoxicity against MCF-7 cell line was P. futfuracea. The highest antioxidant activity was also obtained with P. furfuracea. E. prunastri and R. farinaceae had the highest phenolic and flavonoid contents, respectively. Antibacterial activities of the extracts were determined against ten pathogenic bacteria. The most effective antibacterial agent was methanol extract of R. fastigiata. Our findings have revealed the pharmaceutical potentials of tested lichen species.Öğe Effect of breast milk calcium and fluidity on breast cancer cells: an in vitro cell culture study(Mary Ann Liebert, Inc, 2016) Bayram, Recep; Yavuz, Muhsine Zeynep; Benek, Bedri Selim; Bozkurt, Ayşenur Aydoğar; Ucbek, Ali; Özünal, Zeynep Güneş; Gepdiremen, AkçahanAim: The aims of this study were to investigate the effects of calcium at the same concentration as that found in human milk on the viability, proliferation, and adhesion of MCF-7 human breast ductal carcinoma cells by exposing them to calcium at the same frequency as in breastfeeding. Materials and Methods: High-concentration calcium was applied for 30 minutes every 4 hours for 24, 48, and 72 hours. Cell proliferation and viability were measured using a hemocytometer and the MTT cell viability assay. The effects of calcium treatment were evaluated by a comparison among a multiple-, single-dose calcium treatment, and a control group. Results: We show that calcium at the same concentration as that in milk caused a decrease in the number of cells but did not affect cell viability. Conclusions: The results of this study suggest that calcium caused a lowering of the number of cells from the luminal surface of the breast by triggering proliferation under the condition of fluidity. Calcium and fluidity together serve to eliminate breast cancer stem cells during the lactation period. Effects of the other components of milk can be analyzed by the new method developed in this study.Öğe The effect of NF-kappa B and angiotensin II on the proliferation in human breast adenocancer cell line(2013) Özünal, Zeynep Güneş; Bayram, Recep; Yavuz, Muhsine Zeynep; Ucbek, Ali; Aydoğar, A.; Uzun, Özge; Gepdíremen, Akçahan AkçahanThere are many in-vitro studies implicating that Angiotensin (Ang)II stimulates solid organ cancer growth. Effect of AngII on cell proliferation can be related to nuclear factor NFkB. The aim of the present study is to examine the effects of pyrithione, an NFkB inhibitor and AngII on breast cancer cell proliferation. MCF-7 is treated with AII (10?M) and NFkB inhibitor, pyrithione sodium (0,1-100?M). Cells are counted and photographed. WST-1 is used to measure viability in 48h after treatment and groups are fluorescent dyed with ethidium bromide. The results of cell count showed that cell proliferation was increased in AngII treated group when compared with control group. However, this increase did not show statistically significance. Cell count was decreased in pyrithione (10 and 100?M) treated group. Morphologic changes were most apparent in 100?M pyrithione group. We concluded that pyrithione alone or in combination with AngII decreased MCF-7 cell proliferation.Öğe Effects of plasma-emulating light emitting diode (LED) versus conventional LED on cytotoxic effects of orthodontic cements as a function of polymerization capacity(Sage Publications Ltd, 2014) Çörekçi, Bayram; Halıcıoğlu, Koray; Irgın, Celal; Hezenci, Yasin; Yavuz, Muhsine ZeynepObjectives: The study was aimed at evaluating, in vitro, cytotoxicity of four resin-based orthodontic cements (RBOC) as a function of degree of conversion (DC) and the light curing unit (LCU) employed on mouse fibroblast (L929). Materials and Methods: Nine samples were manufactured for each group of cements using plasma-emulating light-emitting diode (LED) and conventional LED. Toxicity was assessed by immersing four specimens to culture medium (24 h/37 degrees C) for extracting residual monomer or cytotoxic substance. Cell mitochondrial activity of L929 cell was evaluated using methyl tetrazolium (MTT) test. DC was evaluated by Fourier transform infrared spectroscopy for five samples. Results: Cements, LCUs, and interaction between cements and LCUs were found to play a statistically significant role in cytotoxicity (p < 0.0001). Opal band cement (OPAL) plasma LED was found noncytotoxic (90-100% cell viability). The other RBOC-LCU combinations were slightly cytotoxic (60-90% cell viability). Cements (p < 0.01) and LCUs (p < 0.05) had a statistically significant effect on DC. Conversely, interaction between cement and LCU had no statistically significant role on DC (p > 0.05). OPAL plasma LED displayed the highest levels of DC. The correlations between cell viability and DC were positive for three RBOCs. Conclusion: Therefore, high-intensity LCUs can be said to efficiently affect polymerization, so higher DC rates may achieve higher cell viability rates. Clinical Relevance: Cements and LCUs must be matched to each another to result in higher DC and maximal biocompatibility. Dual cure systems presented relatively high cell survival and higher DC, thus expressing superior to single-cure systems with plasma LED.Öğe Effects of plasma-emulating light-emitting diode (LED) versus conventional LED on cytotoxic effects and polymerization capacity of orthodontic composites(Sage Publications Ltd, 2014) Çörekçi, Bayram; Irgın, Celal; Halıcıoğlu, Koray; Dursun, Saffet; Yavuz, Muhsine ZeynepObjectives: The aim of this study was to evaluate, the cytotoxicity of orthodontic composites in vitro as a function of degree of conversion (DC) and the light curing units (LCU) employed on mouse fibroblast (L929). Materials and Methods: Cured samples of the composites Light bond (Reliance Orthodontic Products, Itasca, Illinois, USA), Ortho bracket paste (Bisco, Schaumburg, Illinois, USA), Opal bond MV (OPAL, South Jordan, Utah, USA), and Transbond XT (3M, Monrovia, California, USA) were prepared. Polymerization was performed with two LCUs: VALO Ortho (Ultradent, South Jordan, Utah, USA) is a third-generation LCU and Elipar S I 0 (3M, USA) is a second-generation LCU. Four samples were immersed in cell culture medium to obtain composite extracts. After incubation of L929 cell cultures with the extracts obtained, cytotoxicity was determined using the methyl tetrazolium test. Fourier transform infrared spectroscopy (FTIR) was used to evaluate DC for five samples. A multivariate analysis of variance (ANOVA), two-way ANOVA, and Tukey's honestly significant difference test were utilized for statistical analyses. Results: Cytotoxicity and DC of all tested composites (p < 0.001) and the interaction between composites and LCUs < 0.01) were significantly different. LCUs had no significant influence on the cytotoxicity and DC of composite materials (p > 0.05). The correlations between cell viability and DC were positive for three composites but statistically insignificant. Conclusion: Composites and LCUs must be matched with one another to result in satisfactory maximal biocompatibility and DC. Opal Bond plasma light-emitting diode combination was a better choice for cell viability. Three composites showed a positive correlation between cytotoxicity and DC. Therefore high-intensity LCUs can be said to efficiently affect polymerization, and so, higher DC rates may achieve higher cell viability rates.Öğe Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line(Tubitak Scientific & Technical Research Council Turkey, 2014) Kaya, Ertuğrul; Bayram, Recep; Yaykaşlı, Kürşat Oğuz; Yılmaz, İsmail; Bayram, Sait; Yaykaşlı, Emine; Yavuz, Muhsine Zeynep; Gepdiremen, Ali AkçahanBackground/aim: Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. Materials and methods: The alpha- and beta-amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 mu g/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test. Results: The alpha-amanitin showed a higher toxicity at 36 h, while the highest inhibition of protein synthesis by the beta-amanitin was observed at 24 h. Conclusion: It was shown that the beta-amanitin may be responsible for toxicity, like alpha-amanitin, in Amanita phalloides mushroom poisoning. The early inhibition of protein synthesis for beta-amanitin might be useful for future experiments and research.Öğe Evaluation of pharmaceutical potential and phytochemical analysis of selected traditional lichen species(SOC STIINTE FARMACEUTICE ROMANIA, 2021) Taş, İsa; Yıldırım, Arzu Birinci; Özkan, Erva; Özyiğitoğlu, Gülşah Çobanoğlu; Yavuz, Muhsine Zeynep; Türker, Arzu UçarLichens have been commonly used in traditional medicine. Biological potential (anti-proliferative, antioxidant and anti-bacterial) and chemical content of five lichen species (E. divaricata, L. vulpina, L. pulmonaria, R. fraxinea and U. florida) were assessed in relation to traditional knowledge. Consistent with folkloric usage, the strongest anti-proliferative activity was observed with L. pulmonaria against human hepatocellular carcinoma (HepG2/C3A) cell line. L. pulmonaria also showed the highest antioxidant capacity. While E. divaricata had the most phenolic content, the highest flavonoid content was determined in L. pulmonaria. All lichen extracts showed the best antibacterial activity against Gram-positive bacteria and only R. fraxinea had a broad spectrum of antibacterial activity. HPLC-DAD analysis revealed that L. pulmonaria and U. florida were the best sources of stictic acid and usnic acid, respectively. Traditional usages of tested lichens were justified with this study and nutraceutical potentials of them were revealed.Öğe A green synthesis of new 3-aryl-4-phenylsulfonyl-5-aminoisoxazoles(Pergamon-Elsevier Science Ltd, 2014) Altuğ, Cevher; Büyükbayram, Muhammet; Kavas, Özge; Yavuz, Muhsine ZeynepThe present work describes a new protocol for the synthesis of 5-aminoisoxazoles using alpha-chlorooximes and 2-phenylsulfonyl acetonitrile via green chemistry routes. The titled 5-aminoisoxazoles 3 were further reacted with 4-nitrobenzoyl chloride to obtain 5-amidoisoxazoles with moderate yields. These heterocyclic compounds were tested in vitro MTT study to investigate inhibitive abilities to some cancer cell lines (C3a, L929, T98g and Mcf-7) and compounds 3a, 3c, 3e and 3h showed noticeable cytotoxic property against four cancer cell lines. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Investigation of antiproliferative effect of Tarantula Cubensis extract against Hepatocellular Carcinoma(Wiley-Blackwell, 2015) Bayram, Recep; Karğı, Ertuğrul; Şit, Mustafa; Erkol, Hayri; Yavuz, Muhsine Zeynep; Bayram, Şaban; Şakar, Hakan; Benek, Bedri Selim; Gümüştekin, KenanÖğe Nöronal ve glial hücre kültürlerinde antidepresan ilaçların olası nörotoksik etkilerinin ve hücre içi meydana getirdikleri olaylarda NF-?B'nin rolünün araştırılması(Bolu Abant İzzet Baysal Üniversitesi, 2013) Yavuz, Muhsine Zeynep; Gepdiremen, AkçahanDepresyon günümüzde çok sık karşılaşılan, ancak fizyopatolojisi tam olarak anlaşılamayan bir hastalıktır. Tedavisinde kullanılan ilaçların etkilerini serotonin ve noadrenalin geri alımını inhibe ederek gösterdikleri düşünülse de, yapılan araştırmalarda depresyonun sadece serotonin ve noradrenalin yetersizliği ile oluşan bir hastalık olmadığı ve aynı şekilde tedavisinde kullanılan ilaçların da antidepresan etkilerini yalnızca bu şekilde ortaya çıkarmadıkları anlaşılmıştır. Yapılan çalışmalarda antidepresan ilaçların apoptotik ve proapoptotik etkilerinin olabileceği gözlenmiştir. Bu çalışmada, antidepresan ilaçlardan fluoksetin, imipramin ve maprotilinin nöronlar ve glial hücreler üzerindeki potansiyel koruyucu ve toksik etkileri; hücrelerin morfolojik değişiklikleri değerlendirilerek, canlılık oranlarının karşılaştırılması için MTT testi yapılarak, apopitozis oranlarının belirlenmesi için floresan boyama yapılarak ve apopitotik belirteçlerden olan kaspaz-3 ve kaspaz-8 miktarları karşılaştırılarak araştırılmıştır. Ayrıca bu ilaçların NF-?B üzerindeki etkileri de ELISA yöntemi ile hücre içi NF-?B ve I?B düzeyleri ölçülerek belirlenmeye çalışılmıştır. Sonuç olarak fluoksetin, hücre içi kaspaz 8 düzeyinde değişiklik yapmadan kaspaz 3 miktarını artırarak hücreyi ölüme götürmüştür. Ancak maprotilin ve imipramin hücre içi kaspaz 8 üzerinde de değişiklik yaparak hücre ölümüne neden olduğu ve bu ölüm şeklinin de apoptoz olduğu gösterilmiştir. Araştırmamızda baktığımız diğer bir parametre olan hücre içi NF-?B düzeyi fluoksetin uygulanan her iki hücre tipinde de artışla sonuçlanmıştır. Ancak imipramin ve maprotilinin özellikle toksik dozlarda NF-?B düzeylerini azalttığı gözlenmiştir. Anahtar Kelimeler: antidepresan ilaçlar, Nöron, Glia, Hücre Kültürü, Apopitozis, MTT, NF-?B.Öğe Possible cytoprotective potential of ruthenium red in evaluation of the rapid apoptotic model induced by H2O2(Asian Journal Of Chemistry, 2011) Bayram, Recep; Yavuz, Muhsine Zeynep; Düzenli, Selma; Gepdiremen, AkçahanIn the present paper, hydrogen peroxide (H2O2) induced an apoptotic process in 1 h and over the doses of 10 mM, while cisplatin did not, in cortical neuronal homogenates of rats. It was proved in DNA fragmentation, MTT and WST-1 assays. Especially, according to WST-1 assay results, apoptotic effect was decided to be very obvious in all tests in the doses of 20 mM of the rapid model of apoptosis of H2O2. Ruthenium red, as a mitochondrial Ca2+ modulator, was tested alone and co-application with H2O2. Without H2O2, at low doses of it, ruthenium red seems to have a slight viability inducing effect in respect to controls in MTT and WST-1, despite that this effect was not observed clearly in DNA fragmentation test. Another point is that the highest dose of ruthenium red (10 M), induces apoptosis, even stronger than the 20 mM H2O2, controversy to the general believing.Öğe Promising medicinal plant Inula viscosa L.: Antiproliferative, antioxidant, antibacterial and phenolic profiles(Mattioli 1885, 2019) Özkan, Erva; Karakaş, Fatma Pehlivan; Yıldırım, Arzu Birinci; Taş, İsa; Eker, İsmail; Yavuz, Muhsine Zeynep; Türker, Arzu UçarIntroduction. Inula viscosa (L.) Aiton (sticky fleabane) is popular medicinal plant in the family Asteraceae. It has been used in traditional medicine in the treatment of cancer, diabetes, hypertension, bronchitis, tuberculosis, wounds, skin diseases, infertility, lung and gastro-duodenal disorders. Methods. Aim of this study was to evaluate in vitro antiproliferative, antioxidant and antibacterial activities of aqueous and methanol extracts of aerial parts of I. viscosa and their phenolic constituents. Antiproliferative activity was evaluated against human breast adenocarcinoma (MCF-7) and human brain cancer (T98-G) cell lines using MTT assay. Antioxidant activity was revealed by using 2,2-diphenyl-1-picrylhydrazil (DPPH) method. Total phenol and flavonoid were determined by using Folin-Ciocaltaeu and aluminum chloride (AlCl3) colorimetric method, respectively. The disc diffusion assay was used to screen for antibacterial activity against 10 bacteria. Phenolic constituents were detected by High Performance Liquid Chromatography-Diode-Array Detector (HPLC-DAD) via chosen ten phenolic molecules (gallic acid, caffeic acid, rutin, luteolin, kaempferol, rosmarinic acid, myricetin, quercetin, coumarin and apigenin). Results. Methanol extract of I. viscosa demonstrated better antiproliferative activity than aqueous extract against MCF-7 and T98-G cell lines. Strong DPPH radical scavenging activity was observed with both extracts. Total phenol and flavonoid content of methanol extract were twice as much as aqueous extract. Only Gram-positive bacteria (S. aureus, S. epidermidis and S. pyogenes) were inhibited by both extracts of I. viscosa. HPLC-DAD analysis of phenolic compounds revealed that I. viscosa was significant source of kaempferol. Conclusion: I. viscosa showed promising antibacterial, antioxidant and anticancer activities, and further studies should be conducted to isolate the active components.Öğe Synthesis of novel triazoles bearing 1,2,4-oxadiazole and phenylsulfonyl groups by 1,3-dipolar cycloaddition of some organic azides and their biological activities(Scientific Technical Research Council Turkey-Tubitak, 2014) Dürüst, Yaşar; Karakuş, Hamza; Yavuz, Muhsine Zeynep; Gepdiremen, Ali Akçahan1,3-Dipolar cycloaddition of 5-azidomethyl-3-p-substituted phenyl-1,2,4-oxadiazoles to phenyl vinyl sulfone and bismaleimide gives rise straightforwardly to 1-((3-(p-substituted) phenyl-1,2,4-oxadiazol-5-yl)methyl)-4-(phenylsul-fonyl)- 4,5-dihydro-1 H -1,2,3-triazoles and bisdihydropyrrolo[3,4-d][1,2,3] triazole-4,6(3 a H, 5 H)-diones. The structures of the new cycloadducts were elucidated by means of IR, NMR (1 H, 1 3 C, 2D), mass spectra, and physical characteristics (mp and R f values). In addition, anticancer activities of the cycloadducts against MCF-7 cells were also investigated.
