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Molecular weight distribution analysis by ultracentrifugation : adaptation of a new approach for mucins
(Elsevier Sci Ltd, 2013) Gillis, Richard B.; Adams, Gary G.; Wolf, Bettina; Berry, Monica; Besong, Tabot M. D.; Kök, Şamil Mehmet
Mucins are the key macromolecular component of mucus, nature's natural lubricant, and one of the most important physical properties is their molecular weight distribution. A new approach for polydisperse polymers was recently published based on sedimentation velocity in the analytical ultracentrifuge and converts a distribution of sedimentation coefficient g(s) vs. s plot into a distribution of molecular weight utilising the power-law or scaling relationship between the sedimentation coefficient and molecular weight, s = kappa(s) M-w(b) where s is the sedimentation coefficient, M-w, is the weight average molecular weight and kappa(s) and bare characteristic coefficients related to conformation. We investigate the possibility of using a large database of previously published values of s an M to define kappa(s) and b for both aqueous solution and aqueous solution supplemented by 6 M guanidine hydrochloride (a solvent which helps to minimise sample degradation). These values are then applied to a study of the molecular weight distributions of preparations of human gastric mucin in the different solvents and at different stages of purification. (C) 2012 Elsevier Ltd. All rights reserved.