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  • Küçük Resim
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    Circulating oxidized low-density lipoprotein and paraoxonase activity in preeclampsia
    (Karger, 2005) Uzun, Hafize; Benian, Ali; Madazlı, Rıza; Topçuoğlu, Mehmet Ata; Aydın, Seval; Albayrak, Mustafa
    Preeclampsia is one of the most frequent complications of pregnancy, however, little is known about its etiology. The objective of this study was to investigate the association of oxidized low-density lipoprotein ( oxLDL) and paraoxonase (PON1) activity in women with either preeclampsia or normotensive (NT) pregnancy. The study groups included 41 pregnant women with preeclampsia and 33 normotensive pregnant women. In all patients maternal serum total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TGs) were measured using enzymatic methods. Serum PON1 activities and malondialdehyde (MDA) concentrations were measured by spectrophotometric methods, and oxLDL was measured by enzyme-linked immunoassay ( ELISA). Serum concentrations of lipid parameters ( TC, LDL, VLDL, and TGs) were significantly higher in preeclampsia compared with NT controls ( p < 0.001, p < 0.05, p < 0.05, and p < 0.001, respectively). Serum concentrations of MDA and oxLDL were significantly higher, while PON1 activity was significantly lower in preeclampsia compared with NT controls ( p < 0.001, p < 0.001, and p < 0.001, respectively). A positive correlation was detected between oxLDL and MDA ( r = 0.876), and a negative correlation was detected between both MDA and oxLDL and PON1 ( r = - 0.837 and r = - 0.759, respectively). Our data demonstrate that preeclampsia is associated with increased oxLDL and decreased PON1 activity. Elevated oxidative stress, oxLDL, dyslipidemia and decreased PON1 activities may cause vascular endothelial damage and contribute to the pathophysiology of preeclampsia.
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    Effects of flurbiprofen and tiaprofenic acid on oxidative stress markers in osteoarthritis: a prospective, randomized, open-label, active- and placebo-controlled trial
    (Elsevier Science Inc, 2005) Tüzün, Şansın; Uzun, Hafize; Aydın, Seval; Dinç, Ahmet; Sipahi, Sevtap; Topçuoğlu, Mehmet Ata; Yücel, Rıfat
    Background: The relationship between oxidative stress and osteoarthritis (OA) has been widely investigated. Serum malondialdehyde (MDA), nitric oxide (NO), and Cu/Zn superoxide dismutase (SOD) levels are useful markers of oxidative stress. Because of the importance of oxidative stress markers in the pathogenesis of OA, treatment might involve modification of these markers to control oxidative stress. Objective: The aim of this study was to compare the effects of 2 conventional NSAIDs on markers of oxidative stress in patients with OA of the knee. Methods: This 3-week, prospective, randomized, open-label, active- and placebo-controlled study was conducted at the Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey Adult patients with clinically and radiographically diagnosed moderate OA of the knee who were previously untreated were enrolled. Patients were randomly assigned to 1 of 3 treatment groups: flurbiprofen 100 mg PO (tablets) BID, tiaprofenic acid 300 mg PO (tablets) BID, or placebo tablets BID. Patients were evaluated using clinical assessment and laboratory testing before treatment (week 0; baseline) and at the end of week 3. The primary end points were the differences in serum MDA, NO, and SOD levels versus placebo. Clinical parameters-pain at rest and on motion-were evaluated using a 10-cm visual analog scale (0 = no pain to 10 = worst pain imaginable). The duration (in minutes) of morning stiffness was recorded by patients, using patient diaries. The differences between treatment groups were assessed using multivariate analysis. Results: Thirty-nine patients (20 women, 19 men; mean [SD] age, 59.0 [11.3] years) were included in the study Mean serum MDA and NO levels were significantly decreased at 3 weeks compared with baseline in the 2 active-treatment groups (all, P < 0.001); these values remained statistically similar to baseline in the placebo group. Serum SOD levels were increased significantly from baseline in the 2 active-treatment groups (both, P < 0.00 1), but not in the placebo group. No significant differences in serum MDA and NO levels were found between the group receiving flurbiprofen and that receiving tiaprofenic acid. Serum SOD levels were significantly higher in the flurbiprofen group compared with the tiaprofenic acid and placebo groups (both, P < 0.0 1). The mean (SD) score for pain at rest was significantly lower at 3 weeks compared with baseline with flurbiprofen and tiaprofenic acid (both, P < 0.00 1), but not with placebo. The mean score for pain on motion was significantly reduced from baseline values only with tiaprofenic acid (P < 0.001). The duration of morning stiffness was significantly shorter at 3 weeks compared with baseline in all 3 study groups (all, P < 0.001). The mean scores for pain on motion and duration of morning stiffness were significantly reduced with tiaprofenic acid compared with placebo (both, P < 0.05). The study had some limitations (ie, small sample size, no blinding, the short duration of the study, and the weak correlation between serum and synovial fluid levels of NO). Conclusions: In this comparison of the effects of 3 weeks of treatment with flurbiprofen 100 mg BID and tiaprofenic acid 300 mg BID in patients with knee OA, both treatments effectively reduced serum MDA and NO levels compared with placebo. Only tiaprofenic acid significantly improved pain at rest and on motion and duration of morning stiffness compared with placebo.
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    Hormonal Modulation of the Proinflammatory Cytokines, Leptin and Plasminogen Activator Inhibitor-1 in Healthy Postmenopausal Women
    (Galenos Yayincilik, 2008) Topcuou, M. Ata; Uzun, Hafize; Aydin, Seval; Albayrak, Mustafa; Vehid, Suphi; Zeybek, Gorkem; Topcuoglu, Deniz
    In this study we aimed to determine the levels of TNF-alpha, IL-6, IL-1 beta, PAI-1, leptin in healthy premenopausal and postmenopausal subjects who were evaluated before and after hormone replacement therapy (HRT). Twenty premenoposal woman with regular cycles and 45 postmenopausal women were included in the study. Twenty-five of the postmenopausal women received conjugated estrogen (CCE) + medroxyprogesterone acetate (MPA) and the other 20 received 17-beta estradiol + norethisterone acetate (NETA). The levels of the above identified cytokines in these two treatment groups were compared with that of the healthy pemenopausal group as well as with each other before and after 6 months of HRT. IL-1 levels were significantly higher in the postmenopausal group before HRT when compared to the premenopausal group. Levels of IL-1 were elevated even more after 6 months of HRT. IL-6 was significantly higher in postmenopausal group before HRT compared to the premenopausal group. After 6 months of HRT there was no significant decrease in levels of IL-6. TNF-alpha was significantly higher in postmenopausal group before HRT compared to the premenopausal group. No significant decrease was seen after HRT. PAI-1 was significantly higher in the postmenaposal group before HRT compared to the premenopausal group. After 6 months of HRT, there was a significant decrease in levels of PAI-1. Leptin levels were not different between the premenopausal and the postmenopausal groups (p=0.97). But leptin levels were higher in the postmenopausal group after receiving HRT for 6 months. Menopausal status and HRT may be the causative factor of biochemical proinflammatory response, lepitn. fibrinolytic process and coagulation in healthy postmenopausal women.