Yazar "Terzi, Hakan" seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Beneficial effect of dexmedetomidine on testicular ischemia - reperfusion injury in rats(Wroclaw Medical University, 2008) Terzi, Hakan; Öztürk, Hülya; Buğdaycı, Güler; Öztürk, HayrettinBackground/Objectives. The present study was designed to determine the beneficial effect of dexmedetomidine (Dex) on torsion-detorsion-induced histopathological changes in experimental testicular ischemia/reperfusion (I-R) injury in rats. Material and Methods. Twenty-one male Sprague-Dawley rats were separated into three groups of seven rats each. A sham operation was performed in group 1 (control). In group 2 (I-R/Untreated), unilateral testicular torsion was performed for 6 h followed by 1 h of detorsion of the testis. In group 3 (I-R/Dex), after performing the same procedures as in group 2, dexmedetomidine was given intravenously. Ipsilateral orchidectomies were performed in all experimental rats for histological examination. The levels of MDA and activities of SOD and CAT were measured in the testicular tissue. Results. MDA levels were higher in group 2 than in group 1 rats and lower in group 3 than in group 2. SOD and CAT activities were higher in group 3 than in group 2 rats. Histopathologically, in the group 2 rats the lesions varied between grades III and IV and edema, congestion, hemorrhage between seminiferous tubules, and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the dexmedetomi-dine-treated group 3 showed grades I and II injury. The testicular injury score was also lower in group 3 rats than in group 2. Conclusions. The results show that dexmedetomidine may play a protective role in reducing injury caused by I-R (Adv Clin Exp Med 2008, 17, 5, 513-518).Öğe Cytotoxic effects of nasal buserelin on nasal mucosal tissue in rabbits(Springer, 2012) Oğhan, Fatih; Apuhan, Tayfun; Terzi, Hakan; Kükner, Aysel; Çoksuer, Hakan; Yılmaz, FahrettinTo investigate the cytotoxic effects of nasal buserelin on rabbit nasal mucosal tissue, twenty-four female rabbits were studied prospectively. The rabbits were divided into 4 groups including 6 rabbits. The rabbits' left noses were included in the all study groups: 150 mu g/puff/day of buserelin acetate was administered topically twice daily during 21, 42 and 63 days. Saline was administered topically twice daily to the left nasal cavity in the control group. The nasal septal mucosal stripe tissue was carefully removed from underlaying cartilage after sedation. HE staining, Masson's trichrome, toluidine blue and TUNEL staining were used to evaluate mucosal changes. Each preparation was investigated via apoptotic cells, and they were accounted. Kruskal-Wallis test was used to evaluate nonparametric comparison of apoptotic cells. Mononuclear cells have been raised in the sub-epithelial connective tissue, nucleuses of epithelial cells in the apical region were pyknotic, and apoptotic cells were determined on 21-day group. In the 42-day group, nasal epithelial tissue was similar to 21-day group and epithelial cells including pyknotic nucleus were present in this group, too. In the 63-day group, epithelial cells were light colored. Venous sinuses in the sub-epithelial connective tissue were wide but not congested and not raised collagen filaments. In the intra-epithelial tissue, some of cells were TUNEL (+). Apoptotic cells were fewer in the control group according to 21-day group. In 42- and 63-day groups, these cells were fewer than in 21-day group. Numerical difference was present between the groups, but statistical significance was not found between the groups. We concluded that nasal buserelin cytotoxicity was not potent in the nasal cavity in rabbits. We use nasal buserelin in all indications with confidence.Öğe The effect of pinealectomy and leptin hormone on the proliferation and apoptosis activation in Syrian hamster testis in different photoperiods(Wiley-Blackwell Publishing, Inc, 2009) Gündüz, Bülent; Karakaş, Alper; Terzi, Hakan; Öner, Jale; Serin, Erdinç; Kükner, AyselP>The effects of pinealectomy and leptin hormone on proliferative and apoptotic processes in the epithelia of testicular seminiferous tubules of Syrian hamsters have been investigated. Proliferative and apoptotic processes were assessed semi-quantitatively by proliferating cell nuclear antigen (PCNA) and caspase-3 immune stainings. Animals used in the study were divided into four groups; control, pinealectomy (PinX), leptin-treated (10 mu g/mL/day/kg body weight, intraperitoneally) and pinealectomy + leptin groups. Half of the hamsters in each group were exposed to short and the other half to long photoperiods for 8 weeks. In short photoperiod, PCNA activity especially in spermatogonia was significantly higher in the pinealectomy and leptin-treated groups compared with the control group. Histological score (HSCORE) value of PCNA in the PinX + leptin group was lower than those of PinX and leptin-treated groups. HSCORE value of caspase-3 in PinX and PinX + leptin groups was increased. In the long photoperiod, PCNA activation in the PinX group was significantly lower than the control group while the differences between the controls and other groups were not significant. The difference between the increases in caspase-3 activity in the PinX and control groups was significant. Thus, it was observed that photoperiods had no effect on the proliferation activity in the control groups. The inhibiting effect of short photoperiod on testis was not observed throughout 8 weeks. PinX eliminated the inhibiting effect of short photoperiod but did not alter the stimulating effect of long photoperiod. Leptin did not show any effect in long photoperiod but decreased proliferation by stimulating melatonin in short photoperiod.Öğe Exogenous melatonin treatment reduces hepatocyte damage in rats with experimental acute pancreatitis(Wiley, 2010) Çöl, Cavit; Dinler, Kahraman; Hasdemir, Oğuz; Büyükaşık, Oktay; Buğdaycı, Güler; Terzi, HakanBackground/purpose The hormone melatonin affects cellular immunity in particular and the immune system in general both directly and indirectly. We report our evaluation of the effects of decreasing and increasing serum melatonin levels on hepatocyte damage in rats with experimental acute pancreatitis. Methods Winstar Albino rats with experimentally induced acute pancreatitis were divided into three groups of ten rats each: (1) control (induced acute pancreatitis only); (2) rats with induced acute pancreatitis plus surgical pinealectomy (no melatonin injections); (3) rats with induced acute pancreatitis plus injections of exogenous melatonin. The effects of melatonin levels were evaluated using biochemical and histopathological parameters. Results Rats undergoing the pinealectomy had increased amylase and lactate dehydrogenase (LDH) levels, while those receiving injections of exogenous melatonin had decreased amylase, aspartate transaminase, LDH, and bilirubin levels but increased levels of alanine transferase levels. Conclusion Melatonin may have a therapeutic or protective effect on acute pancreatitis and obstructive jaundice.Öğe Mineralization in the syrinx and caudal tracheal rings in the ostrich(Agricultural Research Communication Centre, 2018) Atalgın, Şükrü Hakan; Ateş, Sevinç; Kürtül, İbrahim; Terzi, HakanThis study documented macroscopic and microscopic features and mineralization of the caudally located tracheal rings and syrinx in two ostrich (struthio camelus) having three years of age. The syrinx and trachea of the birds were stained in toto with alcian blue and alizarin red for cartilage and mineralization. Observations on the syrinx and trachea, measurements and photography were performed under stereo-microscopy. They were stained grossly using alizarin red and alcian blue to visualize mineralization and histologically by hemotoxylin and eosin (H&E) to detect ossification areas, if any. Results revealed incomplete tracheal rings located caudally in-between the intact ones. Alizarin red and alcian blue staining displayed mineralized regions grossly. Histological slides by H&E staining showed no ossification. Overall results proposed that alizarin red and alcian blue double staining is a good toll to determine mineralization which is calcium accumulation in the tissue before the formation of bone cells.Öğe Montelukast protects against testes ischemia/reperfusion injury in rats(Canadian Urological Association, 2010) Öztürk, Hülya; Öztürk, Hayrettin; Gideroğlu, Kaan; Terzi, Hakan; Buğdaycı, GülerIntroduction: In this study, we investigate the effect of montelukast on histologic damage induced by testicular torsion-detorsion in rats. Methods: Twenty-one male Sprague-Dawley rats were separated into 3 groups, each containing 7 rats. A sham operation was performed in group 1 (control). In group 2 (ischemia-reperfusion [I-R]/untreated), 1-hour detorsion of the testis was performed after 6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine), after performing the same surgical procedures as in group 2, montelukast was given intraperitoneally. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and tissue malondialdehyde (MDA), glutathione and myeloperoxidase assays. Results: Montelukast treatment significantly decreased the I-R-induced elevation in testes tissue MDA and glutathione levels were found to be preserved. The level of myeloperoxidase (MPO) activity was significantly increased in the testes tissue of the I-R/untreated group. However, in I-R/montelukast treatment group significantly decreased testes tissue MPO level. Histopathologically, the in the group 2 rats, edema, congestion, hemorrhage between seminiferous tubules and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the montelukast treated group 3 showed grades-I and II injury. Additionally, the testicular injury score was lower in group 3 rats compared with group 2. Conclusion: The current findings demonstrate that the montelukast decreased the severity of testicular injury by reversing the oxidative effects of testes I-R.
