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Öğe A Case of Giant Hepatic Hydatid Cyst Infected with Morganella morganii and the Literature Review(Hindawi Ltd, 2012) Hakyemez, Ismail Necati; Sit, Mustafa; Aktas, Gulali; Tas, Tekin; Mengeloglu, Firat Zafer; Kucukbayrak, AbdulkadirHydatid cyst disease is a common worldwide zoonosis. Most of the cysts are located in the liver. Abscess formation due to infection of the cyst is an important complication. M. morganii, a Gram-negative Bacillus, is a quite rare cause of liver abscess. A 77-year-old woman was admitted to hospital with complaints of fever, chills, nausea, vomiting, loss of appetite, and abdominal pain located in the right-upper quadrant. Her history was positive for hepatic hydatid cyst disease ten years ago. Physical examination revealed a painful mass filling the right-upper quadrant and extending down to umbilicus. Indirect hemagglutinin test for hydatid cyst was positive at a titer of 1/320. Giant liver abscess due to infected hydatid cyst was found in computed tomography scan. Surgeons performed cystectomy and cholecystectomy. Cefazoline, cefuroxime, and metronidazole were administered empirically, but all the three agents were replaced with intravenous ceftriaxone after M. morganii was isolated from the cultures of the abscess material. Clinical signs of the patient resolved at the second week of treatment, and she was discharged.Öğe Evaluation of psychiatric morbidity and quality of life in inactive HbsAg carriers(Academic Journals, 2011) Kucukbayrak, Abdulkadir; Canan, Fatih; Alcelik, Aytekin; Aktas, Gulali; Kocoglu, Esra; Tas, TekinHepatitis B virus (HBV) infection develops in forms from asymptomatic carrier to cirrhosis. There are several studies examining the quality of life of patients with HBV in literature. However, only two studies have investigated the psychiatric morbidity in hepatitis B patients. In this study, we evaluated psychiatric morbidity and quality of life in inactive HbsAg carriers. This case-controlled study was performed among 26 healthy volunteers as control group and 31 inactive HbsAg carriers. Seven (22.5%) of our Hepatitis B patients were found to have an Axis I psychiatric diagnosis. For the carriers, the diagnoses made were major depression (3 patients), major depression with conversion disorder (1 patient), moderate depressive disorder (1 patient), cyclothymic disorder (1 patient), and obsessive-compulsive disorder (1 patient). No significant difference was found between carriers and controls in terms of quality of life scores and anxiety and depression scores. According to these findings, the patients with hepatitis should undergo psychiatric evaluation by a specialist. Also, physicians should be aware that emotional and psychiatric support is not necessary for all of the patients with hepatitis B.Öğe Impact of Storage Time of Fresh Serum Samples on Rapid Test Results of HBsAg(Galenos Yayincilik, 2014) Mengeloglu, Zafer; Bucak, Ozlem; Kocoglu, Esra; Tas, Tekin; Karabork, SeydaObjective: Rapid tests, amongst the methods used in the diagnosis of hepatitis B virus infection, detect hepatitis B surface antigen (HBsAg). It is important to know whether the elapsed time between sample collection and the process has negative impact on the test results. It was aimed to evaluate the impact of samples stored either at room temperature (RT) or at +4 degrees C for different durations on rapid test results. Materials and Methods: A total of 51 serum samples were used. HBsAg tests were performed at the time the samples arrived at our laboratory using chemiluminescence method. 21 samples positive for HBsAg were accepted as the study group, and 30 negative samples were the controls. All the samples were tested immediately using rapid assay. Then the samples were divided into two aliquots and divided again into two groups; the first group was stored at RT, and the second was stored at +4 degrees C. Two or three hours after the first tests, all the samples were tested again using rapid assay, and then, they were continued to be stored; and after 24 hours, the tests were repeated for the third time. Test results were scored between negative and +++. Results: In the initial rapid tests, the sensitivity rate was 85.7%, and the specificity was 100%. A statistically significant association was found between the positivity grades and the mean HBsAg levels (p<0.001; r=0.831). The accuracy was found to be 96.1% (49/51) for the second hour tests in terms of positivity grades. It was observed that the accuracy rate was decreased to 84.3% (43/51) in samples stored for 24 hours in both conditions, and positivity grades of eight samples were decreased for one degree for each. Amongst all tests, no false negative and false positive results were obtained according to the initial tests. In addition, it was found that all the results of the samples stored either at RT or +4 degrees C overlapped, and that the difference was caused just by the duration of storage. Conclusion: In conclusion, sera should not be stored for long time in cases they will be tested using HBsAg rapid assays, and storage of the samples for one day decrease the reliability of the results.Öğe Investigating the Relationship Between HBV DNA Levels and HBV Serological Markers(Galenos Yayincilik, 2013) Kocoglu, Esra; Tas, Tekin; Mengeloglu, Firat Zafer; Karabork, Seyda; Ceylan, KubraObjective: In this study, it is aimed to investigate the relationship between the HBV DNA positivity and serological markers such as HBsAg, HBeAg and anti-HBe in patients with acute or chronic hepatitis. Materials and Methods: Data of 574 patients whose serum specimens were processed in microbiology laboratory between march 2010 and july 2012 were retrospectively analysed. Results: In the study, HBV DNA was detected in 44.8% of HBsAg-positive, in 67.1% of HBeAg-positive and in 35.9% of anti-HBE-positive patients. HBV DNA was detected in 61.2% of the patients both of whose HBsAg and HBeAg were positive. However, HBV DNA was positive in one of 29 patients (3.4%) who had negativity of both HBsAg and HBeAg. DNA positivity was 6.3% in HBsAg-negative patients and 37.7% in HBeAg-negative ones. In the study, HBeAg was negative in 79.4% of HBV DNA-positive patients; anti-HBeAg was negative in 17.7% of DNA-positive patients. DNA levels were found significantly high in HBeAg-positive patients (p<0.001) as well as DNA levels were significantly high in anti-HBenegative ones (p<0.001). Conclusion: In conclusion, serological markers may be insufficient either in diagnosis of HBV infection or in determining the viral replication. In this study, it is observed that evaluating those tests together is useful in laboratory diagnosis.Öğe Performance of Rapid Test in Detection of Anti-HCV in Frozen Sera(Galenos Yayincilik, 2014) Mengeloglu, Zafer; Bucak, Ozlem; Kocoglu, Esra; Tas, Tekin; Karabork, SeydaObjective: Hepatitis C is a common infectious disease throughout the world. Rapid test of hepatitis C, detecting anti-HCV, is a useful test that is easy to perform as well as it may have lack of sensitivity and specificity. In the present study, it was aimed to investigate whether the rapid test of HCV infection can still reveal accurate results in anti-HCV-positive sera frozen for a few years. Materials and Methods: Sera of a total of 100 patients that were determined to be positive for anti-HCV using chemiluminescence technology system (Architect i2000sr, IL, USA) were stored at -200 degrees C for a maximum period of 3.5 years. The sera were thawed and tested again for anti-HCV using the Nanosign rapid test kit (Bioland, Korea) for HCV infection. Results: The positivity rate of the test was 37%. In addition, the rapid test revealed a very low positivity rate as 5.2% in sera with a level of anti-HCV below 10.0 S/CO. In contrast, the positivity rate was 71.4% in samples with high anti-HCV levels. A significant positive correlation was found between positivity levels and anti-HCV levels (r=0.708, p<0.001). No correlation was found between positivity levels and time passed after freezing the samples (r=-0.91, p=0.367). Besides this, no significant difference was observed amongst the groups in terms of the time intervals of freezing (p>0.05). Conclusion: The findings of this study suggest that the rapid test of HCV infection is not reliable in frozen sera with low anti-HCV levels due to the instability of the molecules in the samples, and the time passed after freezing of the sample doesn't change the results of the tests.Öğe A Rare Cause of Pneumonia: Shewanella putrefaciens(Ankara Microbiology Soc, 2012) Tas, Tekin; Kucukbayrak, AbdulkadirWe read with interestingly the paper named A Rare Cause of Pneumonia: Shewanella putrefaciens presented by Durdu et al. published in Mikrobiyol Bul 2012; 46(1): 117-21. S.putrefaciens is a gram-negative, facultative anaerobic and non-fermentative rod that rarely causes infections in humans. First, the authors reported that antibotic sensitivity tests of S.putrefaciens in the presented case were performed by Kirby-Bauer disc diffusion method. However, there is no recommendation about the antibiotic susceptibility testing by disc diffusion test for this bacteria in Clinical and Laboratory Standards Institute (CLSI), European Committee on Antimicrobial Susceptibility Testing (EUCAST), and British Society for Antimicrobial Chemotherapy (BSAC) guidelines. According to CLSI criteria, antibiotic susceptibility tests for S.putrefaciens should be done by a method which detects minimal inhibitory concentration. Second, this paper reported that S.putrefaciens was sensitive to third generation cephalosporins and penicillins. Besides, the authors suggested that susceptibility of these bacteria to these antibiotics was helpful for the differential diagnosis of Pseudomonas aeruginosa and S.putrefaciens. However, in the literature, S.putrefaciens had been reported as resistant to penicillin. We thought that these additional information would be helpful in the future studies related to S.putrefaciens.
