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    Assessment of toxicity and oxidative dna damage of sodium hypochlorite, chitosan and propolis on fibroblast cells
    (Sociedade Brasileira De Pesquisa Odontologica, 2018) Aydın, Zeliha Uğur; Akpınar, Kerem Engin; Hepokur, Ceylan; Erdönmez, Demet
    The objective of this study was to evaluate and compare the cytotoxicity and genotoxicity on human fibroblast cell lines of sodium hypochlorite (NaOCl), chitosan and propolis as root canal irrigating solutions. Human fibroblast cells were exposed to chitosan, propolis and NaOCl for 4 and 24 h. Cell viability was assessed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, and oxidative DNA damage was assessed by determination of 8-hydroxydeoxyguanosine (8-OHdG) level with an ELISA kit. The data of cell cytotoxicity were analysed statistically using a test of one-way analysis of variance at a significance level of p < 0.05. In the NaOCI group, the 8-OHdG level was higher than in the chitosan group, but there was no statistical difference when compared with the other groups (p < 0.05). It was determined that the irrigation solutions were cytotoxic, depending on the dose and time. NaOCl was the most toxic solution after both 4and 24 h of exposure (p < 0.05). Chitosan and propolis may be alternatives to NaOCl for irrigation solutions, because they are both less toxic and produce less oxidative DNA damage.
  • Küçük Resim Yok
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    Effect of various irrigation solutions on cytokine expression of human gingival fibroblast: in vitro study
    (2019) Aydın, Zeliha Uğur; Akpınar, Kerem Engin; Hepokur, Ceylan; Alpay, Merve; Altunbaş, Demet
    Aim: The aim of this study is to investigate the release of IL-1, TNF- ? and VEGF following administration of NaOCl, propolis and chitosan solutions on human gingival fibroblasts. Materials and Methods: This study was conducted on human fibroblast by cell culture and evaluation of the direct effect of various solutions on the cultured cells. The release of pro-inflammatory interleukin (IL), tumor necrosis factor (TNF) ? and vascular endothelial growth factor (VEGF) on fibroblast was analyzed after administration of irrigation solutions. Log concentrations of NaOCl, Propolis and Chitosan effects on cells were measured by colorimetric method. Results: IL-1 and TNF-? secretion levels decreased during propolis and chitosan applications, which are natural products. It was also found that the propolis increased VEGF secretion more than the other materials. Conclusions: The results of this study suggest that propolis and chitosan may contribute to the recovery of periapical tissues via anti-inflammatory cytokines level secreted during the inflammatory process. It is important to search for the biological effect of the materials in contact with the direct or indirect cause of the surrounding tissues during the endodontic treatment.
  • Küçük Resim Yok
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    EVALUATION OF CYTOXICITY OF QMIX, ETHYLENE DIAMINTETRAACETIC ACID AND CHLORHEXIDINE ON HUMAN OSTEOBLAST CELL LINE
    (2018) Aydın, Zeliha Uğur; Akpınar, Kerem Engin; Hepokur, Ceylan; Altunbaş, Demet
    Objectives: In this study, the time-dependent toxic effects of QMix ™, ethylene ediaminetetraacetic acid and chlorhexidine irrigation solutions on human osteoblast cells were as evaluated. Methods and Materials: Human osteoblast cells were grown as monolayer cultures at 37ºC in an atmosphere of 5% CO2 in air and 100% relative humidity. Cells were exposed to ethylene diaminetetraacetic acid (EDTA), chlorhexidine (CHX) and QMix™ for 4 hours and 24 hours. Cell viability was assessed by a 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide kit (XTT) assay. The differences in the mean viability of human osteoblast cells were evaluated statistically. Results: There was a statistically significant difference between the mean percentage of viable cells in the test solutions and control group, both after 4 hour (p?0.001) and 24 hour exposure (p=0.004). The mean percentage of viable cells decreased statistically significantly with the increase in the time of exposure in the EDTA, CHX and QMixTM groups (p?0.05). After 4 hours’ exposure, the EDTA and QMix showed statistically a less toxic effect than did CHX (p?0.05). There was no statistically significant difference between the toxicity of the irrigation solutions after 24 hours’ exposure (p>0.05). Conclusion: All irrigation solutions tested showed various toxic effects on the human osteoblast cell line. The increase in exposure time also increased the toxicity of irrigation solutions on the human osteoblast cell line.