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Öğe Analysis of Antioxidant Effects of Lycopene and Caffeine in Experimentally Induced Renal Ischemia-Reperfusion Injury in Rats(2021) Saylan, Aslihan; Firat, Tülin; Yis, Özgür Mehmet; Dede, Gizem; Carver, HandeBackground: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion (IR) injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were adminis- tered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and se- rum creatinine activities were measured biochemically and light microscopy findings were evaluated histo- logically. Results: When MDA levels are compared; Although the MDA level of the IR+Lycopene group was lower than the IR group, no significance was observed. When IR+Lycopene and IR+Caffeine groups are compared; The MDA level of the IR+Lycopene group is higher than the IR+ Caffeine group. In the IR+Caffeine group; A sta- tistically significant increase was observed in serum creatinine compared to the IR+Lycopene+Caffeine group. Although SOD and GSH-Px were higher in the antioxidant groups compared to the IR group, no sta- tistically significant difference was observed. When compared with ischemia-reperfusion group, histopatho- logical damage was found to be significantly lower than in the other groups. It was found that Lycopene and Caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, des- quame epithelium and proteinous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.Öğe The effect of astaxanthin on human sperm parameters after cryopreservation(Canadian Urological Association, 2022) Dede, Gizem; Saylan, AslıhanIntroduction: The aim of the study was to examine the protecti-ve efficacy of astaxanthin (ASTA) against the damage that occurs during sperm cryopreservation.Methods: This experimental study was carried out on waste semen samples of 30 normozoospermic individuals who applied for semen analysis. Semen samples were divided into four equal volumes and 0 pM (control group), 50 pM, 100 pM, and 500 pM ASTA were added to each group. All groups were stored frozen in a liquid nitrogen tank. Semen samples were removed from liquid nitrogen after 72 hours and were thawed. Motility evaluation of sperm was performed. In addition, sperm was stained with acidic aniline blue to detect DNA chromatin condensation.Results: The highest motility loss was found in the control group and the least motility loss was in the 100 pM ASTA group. When examined in terms of sperm chromatin condensation, condensed sperm count was higher in the 100 pM ASTA group than in the other groups.Conclusions: It has been observed that ASTA added to the cryopro-tectant substance during sperm cryopreservation positively affects sperm motility and reduces the number of decondensed sperm.Öğe Farklı konsantrasyonlardaki astaksantin'in kriyoprezervasyon sonrası insan sperm parametrelerine etkisi(Bolu Abant İzzet Baysal Üniversitesi, 2021) Dede, Gizem; Şaylan, AslıhanÇalışmanın amacı, antioksidan etkisi olduğu bilinen astaksantinin (ASTA) sperm kriyoprezervasyonu sırasında oluşan hasara karşı koruyucu etkinliğini incelemektir. Bu deneysel çalışma, Bolu Abant İzzet Baysal Üniversitesi Tıp Fakültesi'ne semen analizi sebebiyle başvuran 25-40 yaş arası erkek bireylerin atık semen örneklerinde gerçekleştirilmiştir. Dünya Sağlık Örgütü (WHO) 2010 kriterlerine göre; 30 normospermik birey çalışmaya dahil edilmiştir. Kriyoprotektan madde olarak dimetil sülfoksit (DMSO) kullanılan semen örnekleri 4 eşit hacme bölünüp herbir gruba sırasıyla 0 ?M, 50 ?M, 100 ?M ve 500 ?M ASTA eklenmiştir. Tüm gruplar; sıvı azot (LN2/-196°C) tankında dondurularak saklanmıştır. 72 saat sonra sıvı azottan çıkarılan semen örnekleri, çözdürüldükten sonra DMSO ve ASTA uzaklaştırılmıştır. Kriyoprezervasyon öncesi - sonrası spermlerin motilite değerlendirmesi yapılmıştır. Ayrıca spermler, DNA kromatin yoğunlaşmasının tespiti için asidik anilin mavisiyle boyanmıştır. Kriyoprezervasyon sonrasında, kriyoprezervasyon öncesine oranla tüm gruplarda motilite bakımından genel bir düşüş gözlenmiştir. Kriyoprezervasyon sonrası gruplar kendi aralarında karşılaştırıldığında; en fazla motilite kaybına kontrol grubunda (0 ASTA ?M) rastlanırken, en az motilite kaybı 100 ?M ASTA grubunda olmuştur. Bununla birlikte; genel bir düşüş gözlenmesine rağmen ASTA, sperm motilitesini olumlu etkilemiştir. Kriyoprezervasyon sonrası gruplar sperm kromatin kondensasyonu bakımından incelendiğinde ise; kondanse sperm sayısı 100 ?M ASTA grubunda diğer gruplara oranla yüksek çıkmıştır. Elde edilen sonuçlar ve istatistiksel veriler doğrultusunda; sperm kriyoprezervasyonu esnasında kriyoprotektan maddeye eklenen ASTA'nın, sperm motilitesini olumlu yönde etkilediği ve dekondanse sperm sayısını azalttığı görülmüştür. Böylelikle, ASTA'nın kriyoprezervasyon esnasında kriyoprotektanlara ek olarak kullanılabileceği düşünülmektedir. ASTA ile ilgili yeni araştırmaların yapılması, hücre kriyoprezervasyon protokollerinin geliştirilebilmesine ışık tutacaktır.Öğe Protective effect of Coriandrum sativum extract against inflammation and apoptosis in liver ischaemia/reperfusion injury(VIA MEDICA, 2021) Kükner, Aysel; Söyler, Gizem; Toros, Pelin; Dede, Gizem; Meriçli, FilizBackground: The aim of this study was to investigate the anti-inflammatory and antioxidant effects of Coriandrum sativum extract on liver ischaemia reperfusion injury at light microscopic and biochemical levels. Materials and methods: Sham, ischaemia/reperfusion injury (IRI), IRI + Coriandrum sativum extract and only Coriandrum sativum extract groups were formed. Sixty minutes of ischaemia and 60 minutes of reperfusion were performed. In the treatment group, 300 mg/kg/day Coriandrum sativum was given by gavage. Hepatic tissues were fixed in 4% paraformaldehyde. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) enzymes were measured. Nuclear factor-kappa beta (NF-kappa B), tumour necrosis factor-alpha (TNF-alpha) and caspase-3 immunohistochemistry staining was performed. Microscopic scoring was performed in terms of sinusoidal congestion, vacuolisation, and necrosis. Results: Sinusoidal enlargement and diffuse congestion, Kupffer cell increase, neutrophil increase in necrotic areas, vacuolisation in hepatocytes, and bile duct proliferation in the portal triad were observed in ischaemia/reperfusion hepatic tissue. Very rare, necrotic areas were observed in the Coriandrum sativum treatment group, while congestion and vacuolisation and bile duct proliferation were decreased compared to the ischaemic group. The AST and ALT levels were increased in the IRI and IRI + Coriandrum sativum groups. When compared to the IRI group, the AST and ALT levels of the Coriandrum sativum were considerably decreased. The IRI and IRI + Coriandrum sativum groups had statistically significant differences in ALP compared to that of the Coriandrum sativum and Sham groups. There was no significant difference between the ALP levels of the IRI and IRI + Coriandrum sativum groups TNF-alpha, NF-kappa B and caspase-3 immune positive stained hepatocytes were numerous and widely observed in the injury group. There were positive TNF-alpha immunohistochemical staining Kupffer cells in the IRI group. In the group treated with Coriandrum sativum, Kupffer cells were not stained, while TNF-alpha, NF-kappa B and caspase-3 expressing hepatocytes were found to be decreased compared to the IRI group. When the expression values of the TNF-alpha, NF-kappa B and caspase-3 groups were evaluated statistically, it was seen that there was a significant decrease in the group treated with Coriandrum sativum. Conclusions: It was found that Coriandrum sativum extract decreased proinflammatory cytokine TNF-alpha and apoptotic cell death and liver enzymes in liver ischaemia/reperfusion injury.