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Öğe Bond strengths of a self-etching adhesive to dentin surfaces treated with saliva, blood, and different hemostatic agents(Academy of General Dentistry, 2015) Ünlü, Nimet; Cebe, Fatma; Cebe, Mehmet Ata; Çetin, Ali Rıza; Çobanoğlu, NevinThe aim of this study was to evaluate the microtensile bond strengths of a self-etching adhesive to dentin surfaces after treatment with 4 different hemostatic agents in the presence of saliva and blood. After testing, no significant differences were found between the mean bond strength of Clearfil SE (CSE) Bond resin adhesive to normal dentin and those of CSE to dentin treated with the hemostatic agents ViscoStat Clear, Astringedent, or Astringedent X (P > 0.05). However, the mean bond strength of CSE Bond to dentin treated with Ankaferd Blood Stopper (ABS) was significantly greater than those of the other groups (P < 0.05). Thus, while 3 of the tested hemostatic agents did not have significant effects on the bond strength of composite resin to dentin, ABS increased the bond strength of CSE Bond to dentin.Öğe Bonding performance of two newly developed self-adhering materials between zirconium and dentin(Medknow Publications & Media Pvt Ltd, 2015) Cebe, Mehmet Ata; Polat, Serdar; Cebe, Fatma; Tunçdemir, Makbule Tuğba; İşman, ErenPurpose: This study evaluated the effect of four resin materials on the shear bond strength (SBS) of a ceramic core material to dentin. Materials and Methods: Sixty molar teeth were embedded in a self-curing acrylic resin. All specimens were randomly divided into four groups of teeth, each according to the resin cement used. Sixty cylinders were then luted with one of the four resin materials to dentin (GC EQUIA, Panavia F, Variolink II and Vertise). Then, specimens were stored in distilled water at 37 (o) C for one day. Shear bond strength of each specimen was measured using a universal testing machine at a crosshead speed of 0.5 mm/minute. The bond strength values were calculated in N, and the results were statistically analyzed using a Kruskal-Wallis and Bonferroni corrected Mann-Whitney U tests. Results: The shear bond strength varied significantly depending on the resin materials used ( P < 0.05). The specimens luted with GC EQUIA showed the highest shear bond strength (25.19 6.12), whereas, the specimens luted with Vertise flow (8.1 2.75) and Panavia F (11.17 3.89) showed the lowest. Conclusion: GC EQUIA material showed a higher shear bond strength value than other resin materials.Öğe Comparison of eluted monomer from different indirect and direct resin composites(Taylor & Francis Ltd, 2017) Cebe, Fatma; Cebe, Mehmet Ata; Polat, Serdar; Bıçakçı, HasanIndirect resin composites used in indirect restorations have been used as alternative methods to avoid the negative effects of a direct application. The composition and structure of these are similar to a direct composite. Several authors have discussed the elution monomer from resin composites because it is a crucial factor in optimizing the physical properties and biocompatibility of resin composites. The aim of this study was to compare the elution of monomers from different resin composites using high-performance liquid chromatography (HPLC). Indirect composites including Signum, Gradia, and Solidex, and direct composite Filtek Ultimate were used. The samples (2 x 5 mm) were prepared and polymerized for 20 seconds with a light-emitting diode unit. A secondary curing was then applied to the indirect resin composites. After fabrication, each sample was immediately immersed in a 75 wt% ethanol/water solution and analyzed by HPLC. The obtained data were analyzed with one-way ANOVA and Tukey HSD (p < 0.05). Residual monomers were eluted from indirect and direct resin composites and the amount of eluted monomers increased over time. The highest mean concentrations of residual Bis-GMA, UDMA, TEGDMA, and HEMA were detected with the Filtek Ultimate (2,342 mu M), Solidex (7,908 mu M), Signum (2.8 mu M), and Filtek Ultimate (9.7 mu M), respectively. The highest amount of eluted monomer concentrations detected was viewed as critical for toxic reactions in human cells.Öğe Effect of different contact materials on approximal caries detection by laser fluorescence and light-emitting diode devices(Mary Ann Liebert, Inc, 2015) Özsevik, Abdul Semih; Kararslan, Emine Şirin; Aktan, Ali Murat; Bozdemir, Esin; Cebe, FatmaObjective: The aim of this in vitro study was to investigate the influence of the adjacent tooth surface on pen type laser fluorescence (LFpen) and light-emitting diode (LED) device readings in detecting approximal caries lesions in permanent teeth. Background data: Early detection of noncavitated dental caries is important, because disease progression can be easily halted at this stage with certain applications, such as fluoride therapy, antibacterial therapy, dietary changes, or low-intensity laser irradiation. Materials and methods: A total of 87 permanent molars with 156 approximal surfaces were assessed with LED- and LF-based devices in contact with sound tooth surfaces (the control group) as well as approximal amalgam, composite, zirconia, and full ceramic restorations. All teeth were assessed once by one trained examiner. After the LF and LED assessments, the teeth were histologically evaluated using stereomicroscopy as the gold standard. The sensitivity, specificity, accuracy, and area under the receiver operating characteristics (ROC) curve were calculated according to the appropriate thresholds (T1, sound surface or enamel caries; T2, dentin caries). Results: For the LFpen device, higher sensitivity and accuracy was found when the adjacent surface was sound at the T1 threshold and no significant differences were found among accuracy at the T2 threshold. For the LED-based device, no significant differences were found among sensitivities at the T1 threshold. At the T2 threshold, specificity was higher when the adjacent tooth had a zirconia restoration. Conclusions: It was found that both devices could be used effectively for evaluating approximal surfaces with adjacent restored teeth, regardless of the different kinds of restorative materials.Öğe Effect of erbium:yttrium aluminum garnet laser on bond strength of a total-etch adhesive system to caries affected dentin on gingival wall(Wolters Kluwer Medknow Publications, 2017) Cebe, Fatma; Bülbül, Mehmet; Şimşek, İrfan; Cebe, Mehmet Ata; Öztürk, BoraPurpose: To assess the effect of the erbium:yttrium aluminum garnet (Er:YAG) laser on bond strength of a total-etch adhesive system to the caries-affected dentin on the gingival wall. Materials and Methods: Ten human molars with proximal carious lesions were randomly divided into two groups. In the first group, the carious dentin was removed with a bur, whereas in the second group it was removed with the Er:YAG laser. Carious lesions were excavated with one of these two techniques until laser fluorescence values decreased to 15 in the center of the lesions. The teeth were then restored with a total-etch adhesive system (Adper Single Bond 2) and composite resin (Filtek Z250). Five teeth from each group were sectioned to obtain 1 mm(2) stick-shaped microtensile specimens from each tooth. Twenty-five specimens were obtained for each group with using this technique. The data were analyzed in independent-samples t-test (a = 0.05). For each removal technique, one sample was analyzed using scanning electron microscopy. Results: No statistically significant differences were found between the bond strength of the Er:YAG laser and the bur-treated groups (P > 0.05). Conclusion: The Er:YAG laser treatment did not negatively affect the bonding performance of the total-etch adhesive system to caries-affected dentin on the gingival wall.Öğe The effects of different restorative materials on the detection of approximal caries in cone-beam computed tomography scans with and without metal artifact reduction mode(Elsevier Science Inc, 2017) Cebe, Fatma; Aktan, Ali Murat; Özsevik, Abdul Semih; Çiftçi, Mehmet Ertuğrul; Sürmelioğlu, Hatice DeryaObjectives. The aim of this study was to investigate the influence of artifacts produced by different restorative materials on the detection of approximal caries in cone-beam computed tomography (CBCT) scans with and without the application of an artifact-reduction (AR) option. Study Design. Ninety-eight noncavitated premolar and molar teeth were placed with approximal contacts consisting of 2 sound or carious teeth and 1 mesial-occlusal-distal restored tooth with resin-modified glass-ionomer cement (RMGIC), amalgam, composite, ceramic-based composite (CBC), or computer-aided designecomputer-aided manufacturing (CADCAM) zirconia materials in between. The teeth were scanned with a CBCT system with and without the AR option. Images were evaluated by 2 observers. The teeth were histologically evaluated, and sensitivity, specificity, and areas under the receiver operating characteristic (ROC) curve were calculated according to the appropriate threshold. Results. Specificity and sensitivity values for contact surfaces ranged from 0-48.39 and 82.93-98.40, respectively. The AR option affected (P < .05) approximal caries detection of the amalgam, composite, CAD-CAM, and CBC groups in contact surfaces and composite and RMGIC groups in noncontact surfaces. Conclusion. Artifacts produced by different restorative materials could affect approximal caries detection in CBCT scans. Use of the AR option with CBCT scans increases the accuracy of approximal caries detection. (Oral Surg Oral Med Oral Pathol Oral Radiol 2017;123: 392-400)Öğe Elution of monomer from different bulk fill dental composite resins(Elsevier Sci Ltd, 2015) Cebe, Mehmet Ata; Cebe, Fatma; Cengiz, Mehmet Fatih; Çetin, Ali Rıza; Arpağ, Osman FatihObjective. The purpose of this study was to evaluate the elution of Bis-GMA, TEGDMA, HEMA, and Bis-EMA monomers from six bulk fill composite resins over four different time periods, using HPLC. Methods. Six different composite resin materials were used in the present study: Tetric Evo Ceram Bulk Fill (Ivoclar Vivadent, Amherst, NY), X-tra Fill (VOCO, Cuxhaven, Germany), Sonic Fill (Kerr, Orange, CA, USA), Filtek Bulk Fill (3M ESPE Dental Product, St. Paul, MN), SDR (Dentsply, Konstanz, Germany), EQUIA (GC America INC, Alsip, IL). The samples (4 mm thickness, 5 mm diameter) were prepared and polymerized for 20s with a light emitted diode unit. After fabrication, each sample was immediately immersed in 75 wt% ethanol/water solution used as extraction fluid and stored in the amber colored bottles at room temperature. Ethanol/water samples were taken (0.5 mL) at predefined time intervals:10 m (T1), 1 h (T2), 24h (T3) and 30 days (T4). These samples were analyzed by HPLC. The obtained data were analyzed with one-way ANOVA and Tukey HSD at significance level of p < 0.05. Results. Amount of eluted Bis-EMA and Bis-GMA from Tetric Evo Ceram Bulk Fill and amount of eluted TEGDMA and HEMA from X-tra Fill higher than others composites (p < 0.05). Significance. Residual monomers were eluted from bulk fill composite resins in all time periods and the amount of eluted monomers was increased with time. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.Öğe Evaluation of the bond strength between aged composite cores and luting agent(Korean Acad Prosthodontics, 2015) Polat, Serdar; Cebe, Fatma; Tunçdemir, Alirıza; Öztürk, Caner; Üşümez, AslıhanPURPOSE The aim of this study was to evaluate effect of different surface treatment methods on the bond strength between aged composite-resin core and luting agent. MATERIALS AND METHODS. Seventy-five resin composites and also seventy-five zirconia ceramic discs were prepared. 60 composite samples were exposed to thermal. aging (10;000 cycles, 5 to 55 degrees C) and different surface treatment. All specimens were separated into 5 groups. (n=15): 1) Intact specimens 2) Thermal aging-air polishing 3) Thermal aging- Er:YAG laser irradiation 4) Thermal aging- acid etching 5) Thermal-aging. All specimens were bonded to the zirconia discs with resin cement and fixed to universal testing machine and bond strength testing loaded to failure with a crosshead speed of 0.5 mm/min. The fractured surface was classified as adhesive failure, cohesive failure and adhesive-cohesive failure. The bond strength data was statistically compared by the Kruskal-Wallis method complemented by the Bonferroni correction Mann-Whitney U test. The probability level for statistical significance was set at alpha=.05. RESULTS. Thermal aging and different surface treatment methods have significant effect on the bond strength between composite-resin cores and luting-agent (P<.05). The mean baseline bond strength values ranged between 7.07 +/- 2.11 and 26.05 +/- 6.53 N. The highest bond strength of 26.05 +/- 6.53 N was obtained with Group 3. Group 5 showed the lowest value of bond strength. CONCLUSION. Appropriate surface treatment method should be applied to aged composite resin cores or aged-composites restorations should be replaced for The optimal bond strength and the clinical success.Öğe Influence of caries detection dye on composites bond strength of carious affected dentin(Cumhuriyet University Faculty of Dentistry, 2014) Yalçın, Muhammet; Cebe, Fatma; Cebe, Mehmet Ata; Dündar, Ayşe; Öztürk, BoraObjectives: The aim of this in vitro study was to evaluate effect of three different caries detecting dyes on shear bond strength of a self etch adhesive to caries affected dentin. Materials and Methods: 60 extracted human third molars which are affected by caries used in this study. Occlusal surfaces removed to obtain flat dentinal surfaces and teeth mounted acrylic. Specimens were divided into four groups of 15 to be tested three different caries detecting dyes. First group is control and others Caries Detector, Quadrant Cari Test, Sable Seek groups. In control group decayed dentine tissues remowed with silicon carbite papers (300 gride) and in others groups caries detectors applied and carious dentine tissues remowed with silicon carbite papers. Than dentin bonding systems Clearfil SE Bond applied and specimens restorated with composite resin. All products were used according to manufacturer's instructions. Bond strengths were tested and data were statistically analyzed one-way Analysis of Variance (ANOVA) and Tukey tests. Results: As results of this study, Quadrant Cari Test group showed lower bond strength than other detecting dyes groups. While bond strengths of Caries Detector and Sable Seek groups were similar, their bond strengths were significantly higher than Quadrant Cari Test group (p<0,05). Conclusions: According to the results of this in vitro study caries detecting dyes may lead to increased shear bond strength values obtained.Öğe Pulpa perforasyonu ve kanama durdurucu bir ajanın kendinden pürüzlendirmeli bir adeziv sistemin dentine bağlanma dayanımı üzerine etkisi(2014) Cebe, Fatma; Çobanoğlu, NevinAMAÇ: Bu çalışmanın amacı, dentinin Ankaferd Blood Stopper (ABS) ile işlem görmesinin ve pulpa perforasyonununbir kendinden pürüzlendirmeli adeziv sistemin bağlanma dayanımının devamlılığı üzerindeki etkilerinin in vitro olarak incelenmesidir.GEREÇ VEYÖNTEM: Mikrogerilim bağlanma dayanımı testi için40 adet çürüksüz insan 3. büyük azı dişi kullanıldı. DişlereSınıf I kaviteler (bukkolingual genişlik: 3 mm, meziodistalgenişlik: 4 mm, derinlik: 3 mm) açıldı. Kavite açılan dişlerrastgele iki ana gruba ayrıldı ve bir grubun dişlerinde pulpaperforasyonu oluşturuldu. Her iki ana grupta dişlerin yarısıABS ile işlem gördü ve sonra işlem gören ve görmeyen tümörneklere Clearfil Protect Bond (CPB) uygulandıktan sonrakompozit rezin (Clearfil Photo Posterior) ile restore edildi.Tüm gruplardaki örneklerin yarısı 24 saat diğer yarısı ise 12ay sonra değerlendirilmek üzere ayrıldı. On iki aylık gruptaki örnekler nemli ortamda ve 15 cm H2O'luk simüle pulpalbasınç altında saklandı. Saklama süresi sonunda tüm örnekler mikrotensil bağlanma dayanımı testine tabi tutuldu.Verilerin istatistiksel analizi Kruskal-Wallis ve Mann Whitney U testleri ile yapıldı. Grupların ara yüz morfolojileri taramalı elektron mikroskopu (SEM) ile incelendi.BULGULAR: Çalışma sonunda ABS ile işlem gören ve görmeyen gruplar arasında anlamlı fark bulundu (Bonferronidüzeltmesine göre; p<0.0083); ABS, CPB'nin dentine olanbağlanma dayanımını düşürdü. Ancak her iki saklama süresinde de perforasyonlu ve perforasyonsuz gruplar arasında fark bulunamadı (p>0.0083).SONUÇ: Pulpal basıncın taklit edildiği bu sistemde 24 saatve 12 ay bekleme sürelerinde CPB'nin derin dentine bağlanma dayanımında pulpa perforasyonun önemli etkisi görülmedi. ABS temas ettiği dentin üzerinde kalın bir tabakaoluşturmakta ve üzerine doğrudan uygulanan CPB'nindentine bağlanmasına engel olmaktadırÖğe Response of exposed human pulp to application of a hemostatic agent and a self-etch adhesive(Taylor & Francis Ltd, 2015) Cebe, Fatma; Çobanoğlu, Nevin; Özdemir, ÖzgürObjectives: The aim of this study was to investigate the human dental pulp response after use of a hemostatic agent (Ankaferd Blood Stopper [ABS]) and a self-etching adhesive system (Clearfil Protect Bond [CPB]) in direct pulp capping. Materials and methods: For the first aim, 21 non-carious human third molar teeth scheduled for extraction were selected. Class I cavities with pulp exposures were prepared. In group 1, bleeding was controlled with sterile cotton pellets and restored with calcium hydroxide (CH) + ZnOE cement+amalgam. In group 2, bleeding was controlled with sterile cotton pellets and restored with CPB and a composite resin. In group 3, bleeding was controlled with ABS and restored with CPB and a composite resin. The teeth were extracted after 90days, formalin-fixed, and prepared for histological investigation. The pulp response was categorized using the following criteria: inflammatory response, soft tissue organization, reparative dentine formation, and bacterial staining. Data were submitted to statistical analysis, using nonparametric Kruskal-Wallis test. Results: In pulp response study, it showed no statistically significant differences between groups for all parameters (p>0.05). Conclusions: The application of CPB and ABS in direct contact with the mechanically exposed pulp of healthy human teeth can lead to acceptable repair of the dentine-pulp complex, such as wound healing with tertiary dentine bridge formation.Öğe Üç farklı üniversal adeziv sistemin pürüzlendirip yıkamalı ve kendinden pürüzlendirmeli kullanımının makaslama bağlanma dayanımlarının karşılaştırılması(Aves Press Ltd, 2016) Cebe, Mehmet Ata; Bıçakçı, Hasan; Zencirli, Ömer; Cebe, Fatma.Amaç: Bu çalışmanın amacı 3 farklı üniversal adeziv sistemin pürüzlendirip yıkamalı ve kendinden pürüzlendirmeli teknikler ile kullanıldıklarında çürükten etkilenmiş dentinde elde edilen makaslama bağlanma dayanımlarının karşılaştırılmasıdır. Yöntemler: Bu çalışmada 120 adet çekilmiş çürüklü insan büyük azı dişi kullanıldı. Çürük, yüzeyde çürükten etkilenmiş dentine ulaşılana kadar uzaklaştırıldı. Dişler rastgele 6 gruba ayrıldı ve akril kalıplara gömüldü. SBU-s: Single Bond Universal (3M ESPE, St Paul, ABDSBU) kendinden pürüzlendirmeli teknik, ABU-s: All Bond Universal (Bisco Inc, Schaumburg, ABD- ABU) kendinden pürüzlendirmeli teknik, CUB-s: Clearfil Universal Bond (Kuraray Noritake Dental Inc., Okayama, Japonya- CUB) kendinden pürüzlendirmeli teknik, SBU-t: SBU pürüzlendirip yıkamalı teknik, ABU-t: ABU pürüzlendirip yıkamalı teknik ve CUB-t: CUB pürüzlendirip yıkamalı teknik kullanılarak oluşturuldu. Hazırlanan örneklere nanofil kompozit olan Filtek Ultimate Universal Restorative (3M ESPE, St. Paul, ABD) ile restorasyonlar yapıldıktan sonra makaslama bağlanma dayanımı testi yapıldı. Veriler tek yönlü varyans analizi (ANOVA) ve bağımsız t testi ile değerlendirildi (α=0,05). Bulgular: Farklı üniversal adezivlerin pürüzlendirip yıkamalı ve kendinden pürüzlendirmeli kullanımları karşılaştırıldığında anlamlı fark görülmedi (p>0,05). Aynı adezivin farklı kullanımları karşılaştırıldığında ise anlamlı fark sadece CUB grubunda gözlendi. Pürüzlendirip yıkamalı kullanımında daha düşük değerler elde edildi (p<0,05). Sonuç: Üniversal adezivler çürükten etkilenmiş dentinde kullanılacaklar ise kendinden pürüzlendirmeli kullanımları tercih edilebilir.
