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    A crystal quartz microbalance biosensor system to detect triple negative human breast cancer cells
    (Mary Ann Liebert, Inc, 2016) Atay, S.; Pişkin, A.; Yılmaz, Fatma; Aktaş, C. Çakiı; Yavuz, H. Alagöz; Denizli, A.
    Breast cancer is the most common cancer in women. The highly metastatic breast cancer cells are usually difficult to combat with and they usually lead to casualties. Therefore, it may be of value to have a system for early detection of this type of cells. It was aimed to prepare a quartz microbalance biosensor to detect highly metastatic cells, namely MDA-MB 231 cells by targeting their transferrin receptors. The gold surface of the QCM biosensor chip was functionalized by the attachment of transferrin coated Poly(2-hydroxyethyl methacrylate) (pHEMA) nanoparticles. Nanoparticles were characterized by zeta sizer analysis. Nanoparticles without transferrin on their surface were used as control. The QCM sensor systems were analysed by using atomic force microscope (AFM), elypsometer and FTIR-ATR. It was seen that the nanoparticles formed a monolayer over the surface. Following characterization studies the sensors prepared with or without transferrin were tested for their ability to detect invasive breast cancer cells. The binding affinity and kinetics were determined. Adsorbtion kinetics were found to obey Langmuir- Freundlich model. The biosensor was tested with MCF 7 non invasive breast cancer cells and MDA MB 231 cells at prolonged serum deprivation in order to test selectivity. It is concluded that, this biosensor is highly sensitive and selective for vital MDA-MB 231 metastatic human breast cancer cells. Thus, this system may be used to test blood samples for early detection of circulating metastatic breast cancer cells.