Eryaşar, KübraYalçın, Seda Karasu2021-06-232021-06-2320170168-16561873-4863https://doi.org/10.1016/j.jbiotec.2017.06.1027https://hdl.handle.net/20.500.12491/9172European Biotechnology Congress -- MAY 25-27, 2017 -- Dubrovnik, CROATIAXylitol is a five-carbon sugar alcohol widely used in food and pharmaceutical industries as an alternative sweetener. Biotechnological production of xylitol can be achieved by using hemicellulosic hydrolysates of some lignocellulosic materials. In this study, chestnut shell hemicellulosic hydrolysate was used for xylitol production by Candida tropicalis M2. The hydrolysate was prepared by acid hydrolysis followed by evaporation, overliming and active charcoal treatment for detoxification. It was also fortified with xylose and ammonium chloride. Effects of initial xylose concentration, ammonium chloride concentration and shaking rate on xylitol yield were determined by using a central composite design. Experiments were carried out in a batch system using 300 mL cotton-plugged flasks containing 80 mL of fermentation medium. A quadratic model was estimated to be suitable for modelling the experimental data by response surface methodology. Maximum xylitol yield was obtained as 41.7%, which was evaluated as satisfactory since the yields obtained with other hemicellulosic hydrolysates were reported to change between 11 and 84%.eninfo:eu-repo/semantics/closedAccessChestnut ShellHemicellulosic HydrolysateCandida TropicalisXylitolConference Object10.1016/j.jbiotec.2017.06.1027256S67S67WOS:000413585400212Q2