Verma, SandeepYücesan, BuharaGürel, Ekrem2021-06-232021-06-2320100032-0943https://hdl.handle.net/20.500.12491/6685https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0030-12658567th Tannin Conference/58th International Congress and Annual Meeting of the Society-for-Medical-Plant-and-Natural-Product-Research -- AUG 29-SEP 02, 2010 -- Berlin, GERMANYDigitalis lamarckii (Plantaginaceae), is an endemic species of the Turkish flora. D. lamarckii are medicinally important plants as they contain cardiac glycosides that strengthen the cardiac diffusion and regulate the heart rhythm [1]. Hypocotyl sections excised from 21days old seedlings of D. lamarckii were used to test the effect of indole-3-acetic acid (IAA) on direct somatic embryogenesis. Although direct somatic embryogenesis was obtained with all IAA concentrations (0.1, 0.5 and 1mg/l) from the upper part of hypocotyl explants (Fig.1), optimum results were achieved with 1mg/l IAA. Several studies have shown that proper polar transport of auxin is a pre-requisite for normal embryogenesis beyond the globular stage [2]. The auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) blocks morphological transitions to subsequent stages; for example, they cause the formation of enlarged globular and oblong embryos. We observed morphologically abnormal embryos developed on media supplemented with auxin polar transport inhibitors (0.1–2mg/l IAA with 1–5mg/l TIBA). TIBA disrupts somatic embryogenesis and thus specific spatial auxin distribution due to auxin movement may be important in establishing somatic embryo development in D. lamarckii. In the present investigation, contents of five cardenolides as well the total cardenolide contents of the direct somatic embryos were determined, the amount of total cardenolides being 380mg/kg. It has been reported that somatic embryos of the globular stage turned green and, concomitantly, started to produce cardiac glycosides in Digiltalis lanata [3,4].eninfo:eu-repo/semantics/closedAccessDigitalis lamarckii IvanConference Object761213811381WOS:000282066500799Q1