Kurt, Akif HakanOlutaş, Elif BernaAvcıoğlu, FatmaKarakuş, HamzaSungur, Mehmet AliÖztabağ, Cansu KaraYıldırım, Muhammet2024-02-052024-02-052023Kurt, A. H., Olutas, E. B., Avcioglu, F., Karakuş, H., Sungur, M. A., Oztabag, C. K., & Yıldırım, M. (2023). Quercetin-and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities. Beilstein Journal of Nanotechnology, 14(1), 362-376.2190-4286http://dx.doi.org/10.3762/bjnano.14.31https://hdl.handle.net/20.500.12491/11978This study was funded by Bolu Abant Izzet Baysal University, Scientific Research Projects Division (BAP grant no. 2020.08.36.1449) .The presented study comprises the one-pot synthesis and the characterization of quercetin-and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles (Ch/Q-and Ch/CA-Ag NPs), and their antibacterial and anticancer activities. The formation of Ch/Q-and Ch/CA-Ag NPs has been confirmed by ultraviolet-visible (UV-vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The characteristic surface plasmon resonance (SPR) absorption band has been found at 417 and 424 nm for Ch/Q-and Ch/CA-Ag NPs, respectively. The formation of a chitosan shell comprising quercetin and caffeic acid, which surround the colloidal core Ag NPs, was confirmed by UV-vis, and FTIR analyses, and moni-tored by TEM microscopy. The size of nanoparticles has been determined as 11.2 and 10.3 nm for Ch/Q-and Ch/CA-Ag, respec-tively. The anticancer activity of Ch/Q-and Ch/CA-Ag NPs has been evaluated against U-118 MG (human glioblastoma) and ARPE-19 (human retinal pigment epithelium) cells. Both NPs showed anticancer activity, but Ch/Q-Ag NPs seemed to be more effective on cancer cell lines (U-118 MG) in comparison to healthy ones (ARPE-19). Furthermore, the antibacterial activity of Ch/Q-and Ch/CA-Ag NPs against Gram-negative (P. aeruginosa and E. coli) and Gram-positive (S. aureus and S. epidermidis) bacteria was determined, and dose-dependent antibacterial effects were found.eninfo:eu-repo/semantics/openAccessAg NPsAnticancer and Antibacterial EffectsCaffeic AcidChitosanOne-Pot SynthesisQuercetinU-118 MG and ARPE-19 CellsArticle10.3762/bjnano.14.3114362376369982412-s2.0-85153768953Q2WOS:000956622700001Q2