Verma, Sandeep KumarŞahin, GünceYücesan, BuharaEker, İsmailŞahbaz, NevinGürel, SongülGürel, Ekrem2021-06-232021-06-2320120926-66901872-633Xhttps://doi.org/10.1016/j.indcrop.2012.02.034https://hdl.handle.net/20.500.12491/7102This study describes, for the first time, an in vitro protocol for the direct development of somatic embryos and subsequent plant regeneration from hypocotyl segments excised from 21-days-old in vitro-germinated seedlings of Digitalis trojana Ivan (Helen of troy foxglove). Two sets of experiments were carried out, the first compared different concentrations of four cytokinins: N6-benzyladenine [BAP], 6-furfurylaminopurine [kinetin], 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea [TDZ], and 6-(4-hydroxy-3-methylbut-2-enylamino) purine [zeatin] I alone, while the second set tested TDZ or BAP combinations with IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) or NAA (alpha-naphthalene acetic acid). In the first set of experiments, TDZ was found the most effective at 1.0 mg/l concentration, producing a mean of 10.7 somatic embryos per explant. In the second set, a combination of 1.0 mg/l TDZ with 0.5 mg/l IAA produced significantly more somatic embryos per explant (13.8 embryos) than with BAP (8.8 embryos). During subculture on growth regulator-free half-strength MS medium, somatic embryos gradually developed into plantlets. Regenerated plantlets were successfully transplanted and grown in a greenhouse environment. The efficient regeneration protocol reported here provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large-scale production of cardenolides and genetic transformation of this valuable medicinal plant for its further improvement. (C) 2012 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/closedAccessDigitalis TrojanaHelen of Troy FoxgloveHypocotyl SegmentsDirect Somatic EmbryogenesisTissue CultureArticle10.1016/j.indcrop.2012.02.0344076802-s2.0-84858804205Q1WOS:000304744600011Q1