Büyükokuroğlu, Mehmet EminGepdiremen, Ali AkçahanTaştekin, AyhanÖrs, Rahmi2021-06-232021-06-2320070300-9831https://doi.org/10.1024/0300-9831.77.5.336https://hdl.handle.net/20.500.12491/4258In the present study, the possible protective effect of the pyridoxine against glutamate-induced neurotoxicity in cerebellar granular cell culture of rat pups is investigated for its therapeutic potential. Glutamate (10 -7 M) was administered to cerebellar granular cell cultures that were prepared from one-day-old Sprague-Dawley rats. The neuroprotective effect of pyridoxine was examined. Pyridoxine at the doses of 10-8, 10 -7, 10-6, and 10-5 M was introduced into the culture flasks before inclusion of glutamate. Pyridoxine at the doses of 10 -8 M and 10-7 M significantly reduced glutamate cytotoxicity. A 10-7 M dose of pyridoxine proved to be more effective than a 10-8 M dose. The present study demonstrates that pyridoxine may protect glutamate-induced neurotoxicity. Neuroprotective effect of pyridoxine, at least in part, may result from its anti-glutamatergic activity. Pyridoxine merits further investigation as a therapeutic option in hypoxic-ischemic brain injury. © Hogrefe & Huber Publishers.eninfo:eu-repo/semantics/closedAccessCultureGlutamateNeuronPyridoxineRatArticle10.1024/0300-9831.77.5.336775336340184533182-s2.0-38849201219Q3WOS:000253027200005Q4