Yılmaz, Gaye EzgiSaylan, YeserenGöktürk, İlgimYılmaz, FatmaDenizli, Adil2023-09-152023-09-152022Yılmaz, G. E., Saylan, Y., Göktürk, I., Yılmaz, F., & Denizli, A. (2022). Selective amplification of plasmonic sensor signal for cortisol detection using gold nanoparticles. Biosensors, 12(7), 482.2079-6374http://dx.doi.org/10.3390/bios12070482https://hdl.handle.net/20.500.12491/11706Herein, gold nanoparticles (AuNP)-modified cortisol-imprinted (AuNP-MIP) plasmonic sensor was developed for signal amplification and real-time cortisol determination in both aqueous and complex solutions. Firstly, the sensor surfaces were modified with 3-(trimethoxylyl)propyl methacrylate and then pre-complex was prepared using the functional monomer N-methacryloyl-L-histidine methyl ester. The monomer solution was made ready for polymerization by adding 2-hydroxyethyl methacrylate to ethylene glycol dimethacrylate. In order to confirm the signal enhancing effect of AuNP, only cortisol-imprinted (MIP) plasmonic sensor was prepared without AuNP. To determine the selectivity efficiency of the imprinting process, the non-imprinted (AuNP-NIP) plasmonic sensor was also prepared without cortisol. The characterization studies of the sensors were performed with atomic force microscopy and contact angle measurements. The kinetic analysis of the AuNP-MIP plasmonic sensor exhibited a high correlation coefficient (R-2 = 0.97) for a wide range (0.01-100 ppb) with a low detection limit (0.0087 ppb) for cortisol detection. Moreover, the high imprinting efficiency (k ' = 9.67) of the AuNP-MIP plasmonic sensor was determined by comparison with the AuNP-NIP plasmonic sensor. All kinetic results were validated and confirmed by HPLC.eninfo:eu-repo/semantics/openAccessCortisol DetectionGold NanoparticlesMolecular ImprintingPlasmonic SensorSalivary CortisolElectrochemical ImmunosensorArticle10.3390/bios12070482127115358842852-s2.0-85134377559Q1WOS:000833263800001Q1