Çelik, HumeyraÇetinkaya, AyhanBozat, Bihter GökçeDüzcü, Selma ErdoğanAlişik, MuratYis, Özgür Mehmet2024-09-252024-09-2520222630-6425https://doi.org/10.5336/jtracom.2021-83703https://search.trdizin.gov.tr/tr/yayin/detay/533586https://hdl.handle.net/20.500.12491/15771Objective: The effect of wet cupping therapy on anxiety\rand thiol/disulfide homeostasis was evaluated in rats with premature\rovarian failure (POF) induced by cyclophosphamide (CYC). Material\rand Methods: Twenty four Wistar Albino female rats were divided\rinto control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping\r(n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally\rto POF and POF+wet cupping groups. One week later, wet cupping\rtherapy was applied to wet cupping and POF+wet cupping groups for\r5 minutes. The next day, Open Field Test and Elevated Plus Maze Test\rwere applied to all experimental groups to evaluate anxiety. After sacrification,\rto assess pattern formation, endometrial thickness and ovarian\rreserve were measured with hematoxylin-eosin staining.\rThiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione\rlevels were measured in serum to measure oxidative stress. Results:\rAlthough there was no statistical significance, it was observed\rthat anxiety decreased in POF+wet cupping compared to POF group.\rThere was no change in thiol-disulfide values in POF+wet cupping\rcompared to POF group (p>0.05). Conclusion: Wet cupping therapy\rdid not reduce anxiety in the experimental POF model and did not shift\rthe balance to positive in thiol/disulfide homeostasis in favor of thiols.\rHowever, the obtained graphics, numerical data and high standard deviation\rsuggest that meaningful data could be obtained with more subjects.eninfo:eu-repo/semantics/openAccessArticle10.5336/jtracom.2021-83703511221533586