Yoldaş, Meyri ArzuBekdaş, MervanDanış, AyşegülÇetinkaya, AyhanDüzcü, Selma ErdoğanAlışık, MuratKocabey, HüseyinTürel, İdris2024-05-292024-05-292023Yoldaş, M. A., Bekdaş, M., Danış, A., Çetinkaya, A., Düzcü, S. E., Alışık, M., ... & Dinçel, G. K. (2023). Protective and therapeutic effects of okra seed in acute nontraumatic brain injury. International Journal of Neuroscience, 1-10.0020-74541563-5279http://dx.doi.org/10.1080/00207454.2023.2292948https://hdl.handle.net/20.500.12491/12180AimThe purpose of this study was to examine the protective and therapeutic effects of okra (Abelmoschus esculentus [AE]) seed extract, with its known antioxidant, immunomodulatory, and anti-inflammatory properties, in an acetaminophen (paracetamol, N-acetyl- para-aminophenol)-induced model of hepatotoxicity and subsequent acute non-traumatic brain damage.Material and MethodForty male Wistar rats were randomly divided into five equal groups, control, paracetamol (P), okra seed extract (AE), okra seed extract + paracetamol (P + AE), and okra seed extract + paracetamol + N-acetyl cysteine (NAC) (P + AE + N). AE was administered by oral gavage through a gastric tube at 600 mg/kg/day for seven days. On the eighth day of the procedure, a single 1 g/kg dose of paracetamol and 300 mg/kg NAC were injected via the intraperitoneal route 1.5 h after AE administration. Rat tissue specimens were subsequently subjected to biochemical and histopathological analyses. Levels of markers such as S100 calcium-binding protein B (S100B), neuron-specific enolase (NSE), and matrix membrane metalloproteinase-9 (MMP-9) were investigated from rat serum specimens. Malondialdehyde (MDA) and superoxide dismutase (SOD) were also measured to determine oxidant-antioxidant status.ResultsS100B, NSE, MMP-9, MDA levels, and SOD enzyme activities were examined using biochemical methods. MDA levels were significantly lower in the P + AE group and MMP-9 levels in the AE, P + AE, and P + AE + N groups compared to the P group. Histopathological examination results supported the biochemical findingsConclusionOkra seed extract exhibits a protective and therapeutic effect against non-traumatic brain damage resulting from acute paracetamol intoxication. We think that this benefit of AE derives from its antioxidant property.eninfo:eu-repo/semantics/closedAccessAbelmoschus EsculentusAcute Non-Traumatic Brain DamageParacetamolNeuron-Specific EnolaseOxidative Stress; L.AcetaminophenArticle10.1080/00207454.2023.2292948111380605902-s2.0-85179713705Q2WOS:001123448600001Q4