Saylan, AslihanFirat, TülinYis, Özgür MehmetDede, GizemCarver, Hande2024-09-252024-09-2520211304-96231309-4025https://doi.org/10.35440/hutfd.865165https://search.trdizin.gov.tr/tr/yayin/detay/1134300https://hdl.handle.net/20.500.12491/15845Background: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion (IR) injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were adminis- tered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and se- rum creatinine activities were measured biochemically and light microscopy findings were evaluated histo- logically. Results: When MDA levels are compared; Although the MDA level of the IR+Lycopene group was lower than the IR group, no significance was observed. When IR+Lycopene and IR+Caffeine groups are compared; The MDA level of the IR+Lycopene group is higher than the IR+ Caffeine group. In the IR+Caffeine group; A sta- tistically significant increase was observed in serum creatinine compared to the IR+Lycopene+Caffeine group. Although SOD and GSH-Px were higher in the antioxidant groups compared to the IR group, no sta- tistically significant difference was observed. When compared with ischemia-reperfusion group, histopatho- logical damage was found to be significantly lower than in the other groups. It was found that Lycopene and Caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, des- quame epithelium and proteinous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.eninfo:eu-repo/semantics/openAccessKidneyIschemia/Reperfusion InjuryLycopeneCaffeineArticle10.35440/hutfd.8651651835105161134300