Aytürk, NilüferFırat, TülinKükner, AyselÖzoğul, CandanTöre, Fatma2021-06-232021-06-2320171300-01441303-6165https://app.trdizin.gov.tr/makale/TWpVek1UWTFOUT09https://hdl.handle.net/20.500.12491/3119Background/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat. Materials and methods: Four groups were formed as follows: control, Kiss-10 50 nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylineosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined. Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67–stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group. Conclusion: Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period.eninfo:eu-repo/semantics/openAccessKisspeptinSpermatogenesisApoptosisLeydig CellsArticle471334342282635112-s2.0-85014085077Q3253165WOS:000395632600048Q4