Ermurat, YakupÖztürk, MehmetÖnal, CansuKılıçsaymaz, Zekiye2023-11-152023-11-152022Ermurat, Y., Öztürk, M., Önal, C., & Kılıçsaymaz, Z. (2022). Effects of Structural Changes in Bile Salt Hydrolase Enzyme on Biocatalytic Efficiency and Activation Energy at Working pH and Temperature Conditions. Kemija u industriji: Časopis kemičara i kemijskih inženjera Hrvatske, 71(7-8), 413-419.0022-98301334-9090http://dx.doi.org/10.15255/KUI.2021.075https://hdl.handle.net/20.500.12491/11835This work was supported by grants from the Scientific Research Project Office (number 2018.09.02.1271) of Bolu Abant Izzet Baysal University, Turkey.Microbial bile salt hydrolases (BSHs) catalyse the hydrolysis of glycine and taurine-linked bile salts in the small intestine of humans. Achieving the effects of structural changes in BSH molecules on biocatalytic efficiency (kcat/Km) and activation energy (Ea) is necessary to determine biocatalytic performances of the enzymes. Amino acids responsible for biocatalytic activity or substrate specificity in BSH molecules were modified to determine the effects of structural changes on kcat/Km values and Ea values of the bioconversion reactions. Purified wild type positive control enzyme (pCON2) and mutant recombinant target enzymes (F18L and Y24L) reacted with six conjugated pure bile salt substrates at working temperature and pH conditions. The results of the hydrolysis conversion analysis conducted at various pH conditions were used to estimate kcat/Km, and the assays conducted at various temperature conditions were used to approximate Ea of the biocatalytic reactions. The quantified kcat/ Km value was found remarkably highest with mutant recombinant enzymes (Y24L), while the efficiency value with wild type (pCON2) was determined as lowest, indicating that the structural modifications in BSH molecules showed higher values. The alterations with the mutant-type enzymes F18L and Y24L resulted in decreasing kcat/Km and increasing Ea estimations of the hydrolysis conversion reactions.eninfo:eu-repo/semantics/openAccessBile SaltWild HydrolaseRecombinant HydrolaseBiocatalytic EfficiencyCatalytic EfficiencyLactobacillus-PlantarumArticle10.15255/KUI.2021.075717-8413419WOS:000834775700002N/A