Uras, EbruKılıçaslan, DeryaKurt, Akif HakanAllı, BilgeDoğaner, Adem2023-06-232023-06-232021Ebru, U., Derya, K., Hakan, K. A., Bilge, A., & Adem, D. (2021). Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress. Pharmaceutical Chemistry Journal, 55, 762-768.0091-150X1573-9031http://dx.doi.org/10.1007/s11094-021-02490-1https://hdl.handle.net/20.500.12491/11190Kahramanmaras Sutcu Imam University(Kahramanmaras Sutcu Imam University)This study was aimed to determine the efficacy of 6-hydroxy melatonin and N-acetyl serotoninin protecting human retinal pigment epithelial cells from death brought about by oxidative-stress. The ARPE-19 cells (one of the retinal pigment epithelial cell lines) were used as the test cell culture. Following the application of 500 mu M H2O2, ARPE-19 cell line culture was incubated for 20 h to induce oxidative stress damage. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay was used to evaluate the oxidative injury to ARPE-19 cells caused by H2O2 and the effectiveness of 6-hydroxy melatonin and N-acetyl serotonin application in preventing this damage. The results indicated that 6-hydroxy melatonin and N-acetyl serotonin exhibited a therapeutic effect on cells damaged by exposure to oxidative stress. It was concluded that these melatonin metabolites might be useful in treating many retinal conditions linked to the oxidative stress-induced damage. In addition, this study proposed a new idea for developing chemical approaches to the design and synthesis/semisynthesis of new drugs.eninfo:eu-repo/semantics/closedAccess6-Hydroxy MelatoninN-Acetyl SerotoninOxidative StressGamma-Aminobutyric-AcidAntioxidantDamageArticle10.1007/s11094-021-02490-15587627682-s2.0-85119283267Q4WOS:000720203300004Q4