Uçar Türker, ArzuCamper, N. DwightGürel, Ekrem2021-06-232021-06-2320041310-2818https://doi.org/10.1080/13102818.2004.10819230https://hdl.handle.net/20.500.12491/4523An extraction and analytical protocol for saponins were established for Verbascum thapsus L., a medicinal plant. Four different kinds of plant sample were analyzed; field-grown, in vitro cultured, commercially obtained leaves and field-grown capsules. A cleanup procedure with Octadecyl (C18) Solid Phase Extraction Column was used prior to HPLC analysis. Ilwensisaponin A was used as an external standard and digitoxin as an internal standard. C18 reverse phase column and gradient elutions (Acetonitrile with 0.1 % Orthophosphoric acid and Water with 0.1 % Ortho- phosphoric acid) were used for HPLC analysis. Commercially obtained leaves had a higher concentration of saponin (0.215 mg/g tissue) than other leaves (0.081–0.198 mg/g tissue) and capsule sample (0.016 mg/g tissue). © 2004 Taylor and Francis Group, LLC.eninfo:eu-repo/semantics/openAccessChromatographicLiquidArticle10.1080/13102818.2004.1081923018154592-s2.0-1242351927Q3