Ozturk, CESanic, AKaya, DCeyhan, I2024-09-252024-09-2520051344-63041884-2836https://hdl.handle.net/20.500.12491/14318The aim of this study was to use DNA sequencing analysis to analyze the mutations in the most commonly targeted genes (katG, inhA, rpoB, rpsL) in isoniazid (INH)-, rifampin (RIF)- and streptomycin (SM)-resistant Mycobacterium tuberculosis strains obtained from subjects in Duzce, Turkey. Four isolates were found to be INH-resistant, 3 were RIF-resistant and 5 were SM-resistant, out of a total of 52 M. tuberculosis strains. In 3 of the 4 INH-resistant strains, a mutation in the katG gene in codon 315 appeared as AGC -> ACC (Ser -> Thr), and the other INH-resistant strain showed a mutation in the katG gene in codon 314 as ACC -> CCC (Thr -> Pro). There were no mutations in the inhA gene in INH-resistant isolates. Two of the 3 RIF-resistant strains were found to have mutations in the rpoB gene in codon 516 appearing as GAC -> GTC (Asp -> Val), and the other RIF-resistant strain has a mutation in the rpoB gene in codon 531 as TCG -> TTG (Ser -> Leu). These 3 RIF-resistant strains are also INH-resistant. All 5 SM-resistant strains have mutations in the rpsL gene in codon 43 appearing as AAG -> AGG (Lys -> Arg). Thus, we found common gene mutations that bring about the resistance of M. tuberculosis to antituberculosis drugs in all of our isolates from Duzce. To the best of our knowledge, the ACC -> CCC (Thr -> Pro) mutation in the katG gene in codon 314 has not been previously defined.eninfo:eu-repo/semantics/closedAccessArticle58530931216249627WOS:000232992700011Q4